Accumulating evidences indicate that non-coding RNAs (ncRNAs) including long (lncRNAs) and microRNAs (miRNAs) acting as crucial regulators in osteosarcoma (OS). Previously, we reported Rho associated coiled-coil containing protein kinase 1 (ROCK1), a metastatic-related gene was negatively regulated by microRNA-335-5p (miR-335-5p) work an oncogene osteosarcoma. Whether any participate the upstream of miR-335-5p/ROCK1 axial remains unclear. Expression differentiation antagonizing non-protein...

Abstract Background This study was performed to determine the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on granulosa apoptosis and anti-Müllerian hormone (AMH) follicle-stimulating receptor (FSHR) expression in autoimmune drug-induced premature ovarian failure (POF) mice. The aim this research is investigate mechanisms hPMSCs reserve capacity. Methods POF mice model established by injection zona pellucida 3 peptide (pZP3). hPMSC conducted intravenous into...

Long non-coding RNAs (lncRNAs) play key roles in various malignant tumors, including colorectal cancer (CRC). RNA differentiation antagonizing non-protein coding (DANCR) is overexpressed CRC patients, but whether it affects proliferation and metastasis via regulation of heat shock protein 27 (HSP27) remains unclear. In the present study, we found that DANCR was highly expressed correlated with CRC. addition, demonstrated HSP27 were both targets microRNA-577 (miR-577) shared same binding...

Human placenta-derived mesenchymal stem cell (hPMSC) transplantation has been demonstrated to be an effective way of recovering ovarian function in mice with autoimmune induced premature failure (POF). But the exact mechanism remains unclear. The goal present study is investigate role immune factors (T-helper 17 (Th17), cytotoxic T (Tc17) and regulatory (Treg) cells) recovery whether phosphatidylinositol 3-kinase (PI3K)/Akt signal pathway involved regulation.The inhibitor PI3K/Akt was...

Acquisition of drug-resistant phenotypes is often associated with chemotherapy in osteosarcoma. Studies show that high-mobility group box 1 (HMGB1) plays an important role facilitating autophagy and promotes drug resistance osteosarcoma cells. In this study, we determined the targeting miR-22 to HMGB1 regulation on HMGB1-mediated cell proliferation, migration, invasion Results demonstrated well paired 3'-UTR downregulated expression blocked during cells vitro. Further study showed blockage...

MicroRNAs play important roles in tumorigenesis of various types cancers. MiR-320a can inhibits cell proliferation some cancers, but the biologic miR-320a lung cancer need to be further studied. Here, we investigated suppressing adenocarcinoma cells. treatment was found effectively suppress LTEP-a-2 and A549 proliferation, induce more apoptotic cells with irradiation compared control treatment. Our results also showed that miR-320a, as a novel miRNA, directly regulated signal transducer...

Previous studies have shown that the ovarian failure in autoimmune-induced premature (POF) mice could be improved by transplantation of human placenta-derived mesenchymal stem cells (hPMSCs); however, protective mechanism hPMSCs on dysfunction remains unclear. Ovarian is closely related to apoptosis granulosa (GCs). To determine effects GCs apoptosis, an autoimmune POF model was established with zona pellucida glycoprotein 3 (ZP3) peptide. It reported inositol-requiring enzyme 1α (IRE1α) and...

Osteoarthritis (OA) is a common and troublesome disease among the elderly, characterized by extracellular matrix (ECM) degradation. The function of long non‑coding RNA X‑inactive‑specific transcript (XIST) its working mechanism in ECM degradation remains unclear. In present study, XIST was revealed to be upregulated OA specimens articular chondrocytes (ACs) derived from tissue (AC/OA) interleukin‑1β (IL‑1β)‑treated ACs. Loss‑of‑function experiments demonstrated that downregulation suppressed...

Small cell lung cancer (SCLC) accounts for 12 to 16% of neoplasms and has a high rate metastasis. The present study demonstrates the antiproliferative effect retinoic acid amide in vitro vivo against human cells. results from MTT assay showed significant growth inhibition six tested lines clonogenic at 30 μM. Retinoic also leads G2/M-phase cycle arrest apoptosis It caused JAK2, STAT3, STAT5, increased level p21WAF1, decreased cyclin A, B1, Bcl-XL expression. exhibited synergistic on effects...

Background: More and more evidence indicates that microRNAs are present involved in many tumor-related diseases. The function of microRNA-622 (miR-622) colorectal cancer (CRC) remains controversial. Dual specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2) has been reported as a tumor suppressor gene different cancers. detailed regulation mechanism DYRK2 CRC unclear. Methods: miR-622 expression levels were detected at tissue cellular level respectively by using real time...

Signal transducers and activators of transcription (STAT) is a family factors which regulate cell proliferation, differentiation, apoptosis, metastasis, immune inflammatory responses, angiogenesis. STAT3 latent cytoplasmic factor that belongs to STATs. has been reported be regulates genes involved with cellular growth, proliferation metastasis. Worldwide, colon cancer one the leading causes cancer-related deaths. Cumulative evidence established essential for progression advanced malignancy....

Lung cancer is the most common cause of cancer‑associated mortality. MicroRNAs (miRNAs), as oncogenes or tumor suppressor genes, serve crucial roles not only in tumorigenesis, but also invasion and metastasis. Although miRNA‑let‑7a (let‑7a) has been reported to suppress cell growth multiple types, biological mechanisms let‑7a lung adenocarcinoma are yet be fully elucidated. In present study, molecular were investigated by detecting its expression tissues exploring regulation proliferation....

Abstract MicroRNAs (miRNAs) and Smad3, as key transcription factors in transforming growth factor- β 1 (TGF- 1) signaling, help regulate various physiological pathological processes. We investigated the roles of Smad3-regulated miRNAs with respect to lung adenocarcinoma cell apoptosis, proliferation, metastasis. observed that Smad3 phospho-SMAD3 (p-Smad3) were decreased miR-206- (or miR-140)-treated cells there might be a feedback loop between miR-206 miR-140) TGF- expression. Smad3-related...

Tetramethylpyrazine (TMP) is an effective component of the traditional Chinese medicine Chuanxiong, which has been reported to have beneficial effects in various types cancer. However, activity and mechanism action TMP osteosarcoma (OS) not elucidated date. The aim present study was investigate inhibitory effect on OS its underlying action. cells were treated with concentrations for 48 h. BALB/c nude mice intraperitoneal injection at a dose 100 mg/kg every other day 28 days. Cell...

MicroRNA (miR)-335 and Rho-associated serine-threonine protein kinase 1 (Rock1) is ectopically expressed in multiple malignant tumors including osteosarcoma. The present study aimed to clarify whether the combined miR-335 Rock1 was correlated with clinicopathological features prognosis patients expression of 91 osteosarcoma tissue samples 47 noncancerous bone tissues were determined respectively by situ hybridization immunohistochemistry. association between calculated using Pearson's χ2...

Immunosuppression increases disease risk, and the natural compound polydatin (PD) has been reported to modulate immune-related disorders. In cyclophosphamide-induced immunosuppressed mice, PD was evaluated for its immunomodulatory effects. Immune organ indices were measured, while H&E staining ELISA assessed spleen pathology serum cytokine levels. The proliferation of splenic lymphocytes, both total subpopulation, determined using concanavalin A or lipopolysaccharide stimulation, with...