Abstract Background This study was performed to determine the effects of human placenta mesenchymal stem cell (hPMSC) transplantation on granulosa apoptosis and anti-Müllerian hormone (AMH) follicle-stimulating receptor (FSHR) expression in autoimmune drug-induced premature ovarian failure (POF) mice. The aim this research is investigate mechanisms hPMSCs reserve capacity. Methods POF mice model established by injection zona pellucida 3 peptide (pZP3). hPMSC conducted intravenous into...

Recent preclinical studies revealed the efficacy of combined use PI3K inhibitor BKM120 and PARP Olaparib in breast prostate cancers. The current study investigated effect such drug combination on ovarian cancer. Here we showed that inhibition effectively synergized to inhibit proliferation, survival invasion majority cancer cell lines harboring PIK3CA mutations, including SKOV3, HEYA8, IGROV1. Mechanistically, treatment inhibitors resulted an exacerbated DNA damage response more...

Abstract Background Due to metabolic changes in the second trimester and increasing number of pregnant women with obesity advanced maternal age, incidence gestational diabetes mellitus (GDM) remains high. This study aimed evaluate effects GDM on fetal cardiac morphology function, determine whether these increase estimated weight (EFW). Methods Fifty-eight (GDM group) 58 a healthy pregnancy (control were included this prospective observational cohort study. Each group subgroups 31 age between...

Small cell lung cancer (SCLC) accounts for 12 to 16% of neoplasms and has a high rate metastasis. The present study demonstrates the antiproliferative effect retinoic acid amide in vitro vivo against human cells. results from MTT assay showed significant growth inhibition six tested lines clonogenic at 30 μM. Retinoic also leads G2/M-phase cycle arrest apoptosis It caused JAK2, STAT3, STAT5, increased level p21WAF1, decreased cyclin A, B1, Bcl-XL expression. exhibited synergistic on effects...

The present study aimed to investigate the effect and possible mechanism of action crocetin on high cholesterol diet (HCD) induced atherosclerosis rat. Wistar rats were used in current investigation. divided into following group, Group I: control, II: HCD AS, III: AS + (25 mg/kg), IV: (50 mg/kg) V: Simvastatin, respectively. was using vitamin D3 HCD. received pre-determined treatment for 10 weeks. After period, level lipid profile, malonaldehyde (MDA) superoxide dismutase (SOD) also...

Although abnormal activation of the sonic hedgehog (Shh) signaling pathway has been demonstrated in human hepatocellular carcinoma (HCC) patients and most HCC cell lines, mechanism by which Shh promotes development remains uncertain. Using a liver cancer model induced diethylnitrosamine (DEN) mimics process from injury, hepatocyte proliferation, hepatocirrhosis to canceration, we investigated examining expression Shh, patched-1 (Ptch), smoothened (SMO), glioma-associated oncogene-1 (Gli1)...

Abstract Fetal chronic hypoxia is a common pregnancy complication associated with fetal growth restriction. Growth‐restricted offspring have higher risk for liver metabolic disease. Our objective was to better understand how impacts the developing liver. We hypothesized that promotes hepatocellular injury, shifts nutrient metabolism, and activates energetic oxidative stress in used an ovine model of where pregnant ewes were housed under normoxic (CON) or hypoxic (HOX) conditions 30 days late...

Glucose, lactate, and amino acids are major fetal nutrients. During placental insufficiency-induced intrauterine growth restriction (PI-IUGR), uteroplacental weight-specific oxygen consumption rates maintained, yet glucose acid supply is decreased lactate concentrations increased. We hypothesized that metabolism adapts to PI-IUGR by altering nutrient allocation maintain oxidative metabolism. Here, we measured flux rates, with a focus on nutrients shuttled between the placenta fetus...

Purpose: To investigate the influence of cytochrome P450 17α (CYP17A1) gene -34T/C polymorphism in pathogenesis polycystic ovary syndrome (PCOS) Han Chinese population.Methods: Three-hundred eighteen patients with PCOS and 306 controls were recruited CYP17A1 was genotyped using polymerase chain reaction-restriction fragment length (PCR-RFLP). Furthermore, relationship clinical feature parameters also analyzed.Results: The prevalence rates genotype TT, TC CC 49.69%, 43.71% 6.6% case group...

Hepatic fibrosis is a reversible pathological process, in which fibrotic tissue excessively deposited the liver during repair process that follows hepatic injury. Early prevention or treatment of has great significance on chronic diseases. Hydroxysafflor yellow A (HSYA) water-soluble monomer extracted from safflower, serves numerous pharmacological roles. However, it remains to be elucidated how HSYA regulates fibrogenesis. The aim present study was reveal possible mechanisms underlying...

Abstract Background Maternal smoking or passive during gestation increases the risk of congenital birth defects, including neural tube defects (NTDs). Cigarette smoke is a recognized teratogen that causes NTDs, although mechanisms are not well understood. Methods An established model in pregnant golden hamsters was used to observe effect cigarette on development using scanning electron microscopy. Level noggin expression placenta and evaluated by immunohistochemistry. Results Our results...

The antitumor mechanism of curcumin is unclear, especially in hepatocellular carcinoma (HCC) cells. To clarify the action effective treatment HCC, targets were screened and validated. Candidate genes for HCC using traditional Chinese medicine systems pharmacology (TCMSP) database validated Cancer Genome Atlas (TCGA) database. correlation mRNA expression levels between key candidate was identified TCGA liver (LIHC) dataset. effects on prognosis analyzed to identify target gene curcumin, which...

Inflammatory cytokines can destroy the immune balance and lead to adverse pregnancy outcomes. Benzo (a) pyrene (BaP) may induce premature delivery through leading inflammatory reaction. We screened out factors related outcomes bioinformatics analysis. Then we verified correlation between caused by BaP abnormal expression of those factors.The Gene Expression Omnibus (GEO) database was used analyze R screen genes Based on established exposure animal model, key in placenta detected...

This study aimed to observe the regularity of liver cell apoptosis and expression related gene Bcl-2 Bax in induced cancer, explore relationship between development cancer.In this study, 84 rats were used, 72 as experimental group by drinking water containing DEN, 12 control (CG).After laparotomy, form, color, texture metastatic tumor both groups observed recorded.The tissue sectioned stained with hematoxylin-eosin staining demonstrate characteristic morphological changes, Hoechst 33342...

Abstract Objective The aim of the study was to investigate whether ethanolic extract propolis inhibits growth and induces apoptosis HL-60 cells. Methods cells were treated for 24, 48, 72 h with various concentrations extracts (0, 50, 100, 200 μg/mL). proliferation determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Subsequently, Hochest 33258 staining terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) used test We observed expression...

Curcumin is a chemical with various pharmacological activities used for cancer treatment. It inhibits hepatocellular carcinoma (HCC) by inducing apoptosis. Here, the mechanism underlying effect of curcumin on apoptosis HCC cells was studied. Cell counting kit-8 and plate cloning assays were to assess proliferation cells, acridine orange/ethidium bromide Annexin V/PI staining analyze their xenograft tumor models established validate anti-cancer effects curcumin. Expression levels XRCC4...