A5046920069- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Bacterial Identification and Susceptibility Testing
- Pneumonia and Respiratory Infections
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- CRISPR and Genetic Engineering
- Hepatitis C virus research
- Metabolomics and Mass Spectrometry Studies
- interferon and immune responses
- Microbial infections and disease research
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Liver Disease Diagnosis and Treatment
- Medicinal Plants and Bioactive Compounds
- Radiation Dose and Imaging
- Hydrogen's biological and therapeutic effects
- Natural Products and Biological Research
- Heat shock proteins research
- Radioactive contamination and transfer
- Gene expression and cancer classification
- Heavy Metal Exposure and Toxicity
- Vitamin C and Antioxidants Research
Weifang Medical University
2021-2022
Zhejiang University
2010-2016
The Toll-like receptor (TLR)4 complex, TLR4/MD-2, plays an important role in the inflammatory response against lipopolysaccharide, a ubiquitous membrane component Gram-negative bacteria. Ligand recognition by TLR4 initiates multiple intracellular signaling pathways, leading to production of proinflammatory mediators and type I IFN. interaction also leads internalization surface complex into lysosomes, degradation termination LPS response. However, level is maintained via continuous...
PM2.5 is a harmful air pollutant currently threatening public health. It has been closely linked to increased morbidity of bronchial asthma and lung cancer worldwide. Salidroside (Sal), an active component extracted from Rhodiola rosea, reported ameliorate the progression asthma. However, there are few studies on protective effect salidroside PM2.5-induced epithelial cell injury, related molecular mechanism not clear. Here, we aimed explore Sal injury. We chose 50 μg/mL for 24 h as damage...
Abstract The molecular mechanisms that fine tune TLRs responses need to be fully elucidated. Protein phosphatase-1 (PP1) has been shown important in cell death and differentiation. However, the roles of PP1 TLR-triggered immune response remain unclear. In this study, we demonstrate inhibits activation MAPK NF-κB pathway production TNF-α, IL-6 macrophages triggered by TLR3, TLR4, TLR9 a phosphatase-dependent manner. Conversely, knockdown increases TLRs-triggered signaling proinflammatory...
BackgroundShotgun metagenomics has been used clinically for diagnosing infectious diseases. However, most technical assessments have limited to individual sets of reference standards, experimental workflows, and laboratories.MethodsA panel performance metrics were designed examine the shotgun at 17 laboratories in a coordinated collaborative study. We comprehensively assessed reliability, key determinants, reproducibility, quantitative potential.FindingsAssay varied significantly across...
Abstract Background Metagenomic next-generation sequencing (mNGS) offers the promise of unbiased detection emerging pathogens. However, in indexed sequencing, sequential paradigm data acquisition, demultiplexing, and analysis restrain read assignment advance real-time analysis, resulting lengthy turnaround time for clinical metagenomic detection. Methods We described utility internal-index adaptors with different lengths barcode multiplex sequencing. The base composition each position within...
Metagenomic next-generation sequencing (mNGS) can accurately detect pathogens in clinical samples. However, wet-lab contamination constrains mNGS analysis and may result erroneous interpretation of results. Many existing methods rely on large-scale observational microbiome studies not be applicable to tests. By generation a pretrained profile common laboratory contaminants, we developed an noise-filtering model based the inverse linear relationship between microbial reads sample library...
To evaluate the health risk of radon and its progeny, a large amount accurate monitoring data is needed according to theory practice assessment. However, indoor levels in different regions China worldwide reveal temporal spatial variations. In addition, residents living areas follow distinct modes. Therefore, it recommended accepted by many researchers detect level local subsequently conduct assessments based on detection data. this study, 21 bedrooms households Weifang city were selected,...
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated endonuclease Cas13a can specifically bind and cleave RNA. After nucleic acid pre-amplification, bacterial has been used to detect genetic mutations. In our study, using a transcription-mediated amplification together with Cas13a, we isothermally amplify mitochondrial point mutations under non-denaturing conditions from human genomic DNA. Unlike previous reports, prepared CRISPR DNA T7 promoter sequences...
To investigate the renal injury induced by cadmium chloride(CdCl_2) and protective effect of vitamin C(VC) in mice.Forty healthy clean grade male Kunming mice were randomly divided into 4 groups: control group(double distilled water gavage intraperitoneal injection), VC group(200 mg/kg double CdCl_2 2 VC+CdCl_(2 )group(200 injection). Exposure for 30 days.24 hours after last exposure, eyeballs taken out blood, tissue was immediately to calculate kidney coefficient then separate cells. The...
To investigate the damage of rat alveolar type II epithelial cells(RLE-6 TN) caused by air fine particulate matter(PM_(2.5)) and its related mechanism.PM_(2.5) in atmosphere Weifang City 2020 was collected cell culture medium used to prepare suspension. RLE-6 TN cells were exposed different concentrations(25, 50, 100, 200, 400 μg/mL) matter suspensions for 24 h. The morphological changes observed under inverted microscope, viability determined MTT method. concentration lactate...
Abstract Background Clinical shotgun metagenomics for pathogen detection has been used in diagnosing infectious diseases. However, its technical assessments have limited to reference standards by individual labs, single experimental workflow and laboratory. Results Here we reported the design development of a set reagents reporting metrics dedicated clinical National Institutes Food Drug Control (NIFDC) China, joint evaluation study including 17 independent laboratories cross-workflow...
To investigate the protective effect of oleanolic acid(OA) on HgCl_2 induced liver injury.L02 cells were divided into four groups according to different treatment, control group(Con), acid group(OA, 10 μmol/L), group(HgCl_2, 40 μmol/L) and + group(OA HgCl_2). Cells group given serum-free medium, OA pretreated with solution for 8 hours, exposed 6 then hours. MTT assay was used detect cell viability. Laser confocal scanning JC-1 probe fluorescence intensity determine mitochondrial membrane...