A5101776294- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Cell death mechanisms and regulation
- Hippo pathway signaling and YAP/TAZ
- Adipokines, Inflammation, and Metabolic Diseases
- Autophagy in Disease and Therapy
- Phagocytosis and Immune Regulation
- Neurological Disorders and Treatments
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- RNA regulation and disease
- Workplace Health and Well-being
- Obstructive Sleep Apnea Research
- Obesity, Physical Activity, Diet
- Fibroblast Growth Factor Research
- Immunodeficiency and Autoimmune Disorders
- Viral Infections and Immunology Research
- Tracheal and airway disorders
- Neurological and metabolic disorders
- Ocular Oncology and Treatments
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Occupational exposure and asthma
- Nutrition and Health in Aging
Yonsei University
1994-2024
Pusan National University
2020-2021
Pusan National University Hospital
2020-2021
RNA-guided endonucleases (RGENs), derived from the prokaryotic Type II CRISPR-Cas system, enable targeted genome modification in cells and organisms. Here we describe establishment of gene-knockout mice zebrafish by injection RGENs as Cas9 protein:guide RNA complexes or mRNA plus guide into one-cell-stage embryos both species. efficiently generated germline transmittable mutations up to 93% newborn with minimal toxicity. RGEN-induced mouse Prkdc gene that encodes an enzyme critical for DNA...
Obesity has emerged as a prominent risk factor for the development of malignant tumors. However, existing literature on role adipocytes in tumor microenvironment (TME) to elucidate correlation between obesity and cancer remains insufficient. Here, we aim investigate formation cancer-associated (CAAs) their contribution growth using mouse models harboring dysfunctional adipocytes. Specifically, employ adipocyte-specific BECN1 KO (BaKO) mice, which exhibit lipodystrophy due Our results reveal...
Abstract Necroptosis is a form of regulated cell death caused by formation the necrosome complex. However, factors modulating this process and systemic pathophysiological effects necroptosis are yet to be understood. Here, we identified that Beclin 1 functions as an anti-necroptosis factor being recruited into complex upon treatment with TNFα, Smac mimetic, pan-caspase inhibitor repressing MLKL oligomerisation, thus preventing disruption plasma membrane. Cells ablated or knocked-out for...
Few studies have shown the combined impact of sitting time and physical activity on quality life in older people. This cross-sectional study, using data from 2016–2018 Korean National Health Nutrition Examination Survey, examines association between health-related (HRQoL) adults aged ≥ 65 years. HRQoL was assessed EuroQol-5 Dimension (EQ-5D, three-level version). We divided subjects into groups based analyzed with HRQoL. The longer (≥8 h) multiple logistic regression. In total, 4276...
The deficiency of retinoblastoma (Rb) gene deregulates E2F transcription factors and thus induces target genes directly or p53 indirectly via mouse p19Arf (or p14ARF in humans), an gene. Here, we identified that etoposide-induced 2.4 mRNA (Ei24)/p53-induced 8 (Pig8), a involved apoptosis autophagy, was up-regulated Rb−/− embryonic fibroblasts (MEFs). Ei24 promoter activated by E2F1 multiple E2F-responsive elements, independently the previously reported p53-responsive element. Chromatin...
Becn1/Beclin-1 is a core component of the class III phosphatidylinositol 3-kinase required for autophagosome formation and vesicular trafficking. Although Becn1 has been implicated in numerous diseases such as cancer, aging, neurodegenerative disease, role white adipose tissue related metabolic remains elusive. In this study, we show that adipocyte-specific knockout mice develop severe lipodystrophy, leading to inflammation, hepatic steatosis, insulin resistance. Ablation adipocytes...
Upregulation of human telomerase reverse transcriptase (hTERT) expression is an important factor in the cellular survival and cancer. Although growing evidence suggests that hTERT inhibits apoptosis by telomere-independent functions, mechanisms involved are not fully understood. Here, we show contains a BH3-like motif, short peptide sequence found BCL-2 family proteins, interacts with anti-apoptotic proteins MCL-1 BCL-xL, suggesting functional link between mitochondrial pathway apoptosis....
RAG1 or RAG2 mutations are associated with defects in V(D)J recombination activity, causing severe immunodeficiency a wide spectrum of clinical phenotypes. A R229Q mutation was identified patients combined (SCID) Omenn syndrome (OS). Although some factors determining the features between SCID and OS were not clear, molecular mechanism studied mouse model which an EGFP tag is fused to Rag2 mutation. To design human disease mimicking immunodeficiency, we generated Rag2-R229Q knock-in mice...
This study analyzed the relationship between work pattern and prevalence of periodontitis. We data 3320 adults (1779 men, 1543 women) aged 51–80 years from Korean National Health Nutrition Examination Survey (2013–2015). The was divided into two groups (regular irregular). periodontal status assessed using community index. observed a statistically significant difference in association patterns periodontitis women over 50 years. For female workers with irregular patterns, lower than that...
Abstract Engineered nucleases are widely used for creating frameshift or nonsense mutations in the target genes to eliminate gene functions. The resulting mRNAs carrying premature termination codons can be eliminated by nonsense-mediated mRNA decay. However, it is unclear how effective this process would vivo . Here, we found that decay was unable remove mutant twelve out of sixteen homozygous mice with generated using engineered nucleases, which far beyond what expected. proteins translated...
The pathophysiologic changes that result in acquisition of obstructive sleep apnea syndrome (OSAS) are poorly understood. To identify the influence increased pharyngeal compliance and collapsibility to tissue OSAS, transverse sections uvula were compared using electron microscope from 10 OSAS patients group 4 normal control group. obtained results as follows: Muscle nerve fibers controls show appearance. In group, there various degrees muscle such hypertrophy or atrophy degeneration axonal...