A5056556596- Zebrafish Biomedical Research Applications
- Congenital heart defects research
- Angiogenesis and VEGF in Cancer
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- interferon and immune responses
- Lysosomal Storage Disorders Research
- Retinal Development and Disorders
- Cancer-related gene regulation
- Axon Guidance and Neuronal Signaling
- Alzheimer's disease research and treatments
- Calpain Protease Function and Regulation
- RNA Research and Splicing
- Head and Neck Anomalies
- Genetic Syndromes and Imprinting
- Cleft Lip and Palate Research
- Viral Infectious Diseases and Gene Expression in Insects
- Nerve injury and regeneration
- Mosquito-borne diseases and control
- Connexins and lens biology
- Blood Coagulation and Thrombosis Mechanisms
- Cancer, Hypoxia, and Metabolism
- Gestational Trophoblastic Disease Studies
- Fish Ecology and Management Studies
- Chemical Synthesis and Analysis
Matsunami General Hospital
2025
Gifu University
2023
South Australian Health and Medical Research Institute
2016-2020
Centre for Cancer Biology
2020
University of South Australia
2010-2020
National Institutes of Health
2004-2016
University of Maryland, College Park
2016
Eunice Kennedy Shriver National Institute of Child Health and Human Development
2003-2015
University of Auckland
2010
Laboratory of Molecular Genetics
2006
We report here that all trans-retinoic acid (RA), a classical morphogen, induces apoptosis during the neural differentiation of embryonic stem cell line P19. The apoptotic cells showed, in addition to DNA cleavage, typical morphological changes including chromatin condensation, nuclear fragmentation, and cytoplasmic vacuolation. These became obvious by 12 h after RA. endogenous expression bcl-2 surviving was down-regulated this process, compelled retroviral vectors reduced number cells....
The zebrafish is a powerful model for studying vascular development, demonstrating remarkable conservation of this process with mammals. Here, we identify mutant, redhead ( rhd mi149 ), that exhibits embryonic CNS hemorrhage intact gross development the vasculature and normal hemostatic function. We show phenotype caused by hypomorphic mutation in p21-activated kinase 2a pak2a ). PAK2 acts downstream Rho-family GTPases CDC42 RAC has been implicated angiogenesis, regulation cytoskeletal...
The cerebral vasculature provides the massive blood supply that brain needs to grow and survive. By acquiring distinctive cellular molecular characteristics it becomes Blood Brain Barrier (BBB), a selectively permeable protective interface between peripheral circulation maintains extra-cellular milieu permissive for neuronal activity. Accordingly, there is great interest in uncovering mechanisms modulate formation differentiation of vasculature. performing forward genetic screen zebrafish we...
Juvenile polyps (JPs) are non-neoplastic polyps. In adults, JPs present with hematochezia in only approximately half the patients and often found incidentally during endoscopic screening. have no mucosal fascia at tip, spontaneous shedding massive gastrointestinal hemorrhage may occur. Thus, JP bleeding detected this case by extravascular contrast leakage on computed tomography scans treated clipping is rare. A previously healthy 31-year-old male patient presented a 2-day history of bloody...
Eukaryotic elongation factor 2 kinase (eEF2K) negatively regulates the phase of mRNA translation and hence protein synthesis. Increasing evidence indicates that eEF2K plays an important role in survival migration cancer cells tumor progression. As demonstrated by two‐dimensional wound‐healing three‐dimensional transwell invasion assays, knocking down or inhibiting impairs cells. Conversely, exogenous expression eEF2 (the substrate eEF2K) accelerates wound healing invasion. Importantly, using...
Lysosomal vesicles around neuritic plaques are thought to drive Alzheimer's disease by providing ideal microenvironments for generation of amyloid-β. Although lysosomal present at every amyloid plaque in mouse models disease, the number that contain human brain remains unknown. This study aimed quantify hippocampus. Lysosome-associated membrane protein 1 (LAMP1)-positive accumulated both diffuse (Aβ42-positive/AT8-negative) and (Aβ42-positive/AT8-positive) all regions were analysed. In...
A variety of different transgenic zebrafish lines have been generated expressing green fluorescent protein (GFP) or other proteins in organs and tissues, permitting dynamic visualization development these tissues living animals via time-lapse imaging. Although methods devised for short- to medium-term imaging zebrafish, are not suitable longer term because poor control over temperature, evaporation, anoxia. We describe a new chamber that provides continuously circulating flow warm,...
Understanding the mechanisms regulating normal and pathological angiogenesis is of great scientific clinical interest. In this report, we show that mutations in 2 different aminoacyl-transfer RNA synthetases, threonyl tRNA synthetase (tars(y58)) or isoleucyl (iars(y68)), lead to similar increased branching developing zebrafish.The unfolded protein response pathway activated by deficiencies, genes atf4, atf6, xbp1, as well key proangiogenic ligand vascular endothelial growth factor (vegfaa),...
Cardiomyocytes undergo growth and remodeling in response to specific pathological or physiological conditions. In the former, myocardial is a risk factor for cardiac failure faster protein synthesis major driving cardiomyocyte growth. Our goal was quantify rapid effects of different pro-hypertrophic stimuli on proteins ARVC determine whether such are caused by alterations mRNA abundance translation mRNAs. have very low rates synthesis, posing challenging problem terms studying changes...
The premature stop codon mutations, Q70X and W402X, are the most common α-L-iduronidase gene (IDUA) mutations in mucopolysaccharidosis type I (MPS I) patients. Read-through drugs have been used to suppress codons, this can potentially be treat patients who of mutation. We examined effects aminoglycoside treatment on IDUA W402X cultured cells show that 4,5-disubstituted aminoglycosides induced more read-through for mutation, while 4,6-disubstituted promoted mutation: lividomycin...
Abstract We identified four mutants in two distinct loci exhibiting similar trunk vascular patterning defects an F3 genetic screen for zebrafish mutants. Initial vasculogenesis is not affected these mutants, with proper specification and differentiation of endothelial cells. However, all display severe the growth angiogenic vessels trunk, ectopic branching disoriented migration intersegmental vessels. The are allelic to previously characterized at fused ‐ somites ( fss ) beamter bea loci,...