A5090141325- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Malaria Research and Control
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virology and Viral Diseases
- SARS-CoV-2 and COVID-19 Research
- Hepatitis Viruses Studies and Epidemiology
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Vector-borne infectious diseases
- Immunotherapy and Immune Responses
- Axon Guidance and Neuronal Signaling
- Neonatal and Maternal Infections
- Influenza Virus Research Studies
- Infectious Encephalopathies and Encephalitis
- Angiogenesis and VEGF in Cancer
- Escherichia coli research studies
- Infectious Diseases and Tuberculosis
- Sepsis Diagnosis and Treatment
- Heparin-Induced Thrombocytopenia and Thrombosis
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Respiratory viral infections research
Universidad Surcolombiana
2016-2025
Universidad del Valle
2024
Hospital Universitario de Neiva
2014-2024
Secretaría de Salud de Bogotá
2023
Marqués de Valdecilla University Hospital
2022
Universidad de Cantabria
2022
Military University Nueva Granada
2020-2022
Instituto Nacional de Cancerología
2020-2022
Instituto de Investigación Marqués de Valdecilla
2022
Hospital Universitario Puerto Real
2021
During seasonal influenza epidemics, disease burden is shouldered predominantly by the very young and elderly. Elderly individuals are particularly affected, in part because vaccine efficacy wanes with age. This has been linked to a reduced ability induce robust serum antibody response. Here, we show that this due quantities of vaccine-specific antibodies, rather than lack avidity or affinity. We measured levels plasmablasts ELISPOT 1 week after immunization elderly adults inactivated...
A low-cost, equipment-free rapid antigen test distinguishes dengue virus serotypes and Zika in patient sera without detectable cross-reactivity.
B cell-dependent immunity to rotavirus, an important intestinal pathogen, plays a significant role in viral clearance and protects against reinfection. Human vitro murine vivo models of rotavirus infection were used delineate the primary plasmacytoid DCs (pDCs) initiating cell responses. pDCs necessary sufficient for activation induced by rotavirus. Type I IFN recognition cells was essential rotavirus-mediated IFN-α/β-mediated after infection. Furthermore, rotavirus-specific serum mucosal...
The cerebral vasculature provides the massive blood supply that brain needs to grow and survive. By acquiring distinctive cellular molecular characteristics it becomes Blood Brain Barrier (BBB), a selectively permeable protective interface between peripheral circulation maintains extra-cellular milieu permissive for neuronal activity. Accordingly, there is great interest in uncovering mechanisms modulate formation differentiation of vasculature. performing forward genetic screen zebrafish we...
ABSTRACT Background There is scarce evidence on the fourth dose of severe acute respiratory syndrome coronavirus 2 vaccines in chronic kidney disease (CKD) patients. We evaluated humoral response and effectivity CKD spectrum: non-dialysis (ND-CKD), haemodialysis (HD), peritoneal dialysis (PD) transplant (KT) recipients. Methods This a prespecified analysis prospective, observational, multicentric SENCOVAC study. In patients with who had received complete initial vaccination one or two...
Purpose Primary central nervous system (CNS) tumors are the second most common cancer in children and adolescents, leading to premature death disability. Population-based survival estimates aid decision-making control, however data on for primary CNS Latin America is lacking. We describe rates with treated ten Colombian cities. Methods analyzed from adolescents newly diagnosed between 2012 2021, participating Childhood Cancer Clinical Outcomes Surveillance System (VIGICANCER) cities...
Background Gestational Zika virus (ZIKV) infection is associated with the development of congenital syndrome (CZS), which includes microcephaly and fetal demise. The magnitude quality orthoflavivirus-specific humoral immunity have been previously linked to CZS. However, role ZIKV NS1-specific in mothers children prenatal exposure CZS remains undefined. In addition, considering that most at-risk population lives dengue (DENV)-endemic areas, it not clear what association between pre-existing...
Chronic kidney disease-associated pruritus (CKD-aP), a persistent itching sensation, is prevalent among hemodialysis patients. This study aims to understand its prevalence, possible association with selected solutes, and impact on quality of life (QoL) in Involving 434 patients Spain, this observational employed survey based validated assessment tools, alongside demographic, clinical, biochemical data collection. The used statistical analyses examine the correlation between CKD-aP various...
Identifying immune markers driving early and effective antibody response in patients with severe coronavirus disease 2019 (COVID-19) is critical due to the threat of future pandemics, incomplete global vaccination, suboptimal booster coverage. Patients life-threatening acute respiratory syndrome 2 (SARS-CoV-2) infection are characterized by dysregulated thromboinflammation cytokine storm that could influence isotype virus-specific subsequent clinical outcome. We investigated association...
ABSTRACT We have previously shown that very few rotavirus (RV)-specific T cells secrete gamma interferon circulate in recently infected and seropositive adults children. Here, we studied the interaction of RV with myeloid immature (IDC) mature dendritic (MDC) vitro. did not induce cell death IDC or MDC induced maturation between 12 48% IDC. Nonetheless, inhibit change expression markers on MDC. After treatment RV, expressed nonstructural viral protein NSP4. In contrast, a discrete productive...
To evaluate the clinical, laboratory, and immune characteristics of Zika virus (ZIKV)-associated encephalitis in pediatric patients after epidemic Huila, southern Colombia.A neuro-surveillance hospital study was conducted a referral health center Colombia, from October 2016 to 2017. Cases were confirmed by nucleic acid amplification tests serological methods cerebrospinal fluid (CSF), plasma, and/or urine. Levels six cytokines evaluated flow cytometry. Patients underwent daily clinical...
In a double blind trial, 319 fully immunized children received two doses of either placebo or 10(6.7) focus-forming units the attenuated RIX4414 human rotavirus (RV) vaccine ("all-in-one" formulation). Plasma RV-specific IgA (RV IgA), stool RV IgA, and circulating total memory B cells (CD19+ IgD+/- CD27+) with an intestinal homing phenotype (alpha4beta7+ CCR9+/-) were measured, after first second doses, as potential correlates protection. After and/or dose, 54% vaccinees 13% recipients had...
Background Dengue virus (DENV) infections pose one of the largest global barriers to human health. The four serotypes (DENV 1–4) present different symptoms and influence immune response subsequent DENV infections, rendering surveillance, risk assessments, disease control particularly challenging. Early diagnosis appropriate clinical management is critical can be achieved by detecting nonstructural protein 1 (NS1) in serum during acute phase. However, few NS1-based tests have been developed...
Patients on hemodialysis are at high-risk for complications derived from coronavirus disease 2019 (COVID-19). The present analysis evaluated the impact of a booster vaccine dose and breakthrough severe acute respiratory syndrome 2 (SARS-CoV-2) infections humoral immunity 3 months after dose. This is multicentric prospective study assessing immunoglobulin G anti-Spike antibodies 6 9 initial SARS-CoV-2 vaccination in patients that had also received before 6-month assessment (early booster) or...
Protective immunity to rotavirus (RV) is primarily mediated by antibodies produced RV-specific memory B cells (RV-mBc). Of note, most of these express IgM, but the function this subset poorly understood. Here, using limiting dilution assays highly sort-purified human IgM(+) mBc, we found that 62% and 21% total (non-antigen-specific) RV-IgM(+) respectively, switched in vitro IgG production after polyclonal stimulation. Moreover, assays, median cloning efficiencies (17%) (7%) mBc were lower...
Abstract Dengue is a significant health problem due to the high burden of critical infections during outbreaks. In 1997, World Health Organization (WHO) classified dengue as fever (DF), hemorrhagic (DHF), and shock syndrome (DSS). It was revised in 2009 (updated 2015), new guidelines recommended classifying patients without warning signs (DNS), with (DWS), severe (SD). Although utility classification for clinical studies accepted, immunological it needs be clarified. We determined usefulness...
Objective The aim of this study is to determine the utility C reactive protein (CRP) and interleukin (IL)-6 in diagnosis neonatal sepsis (NS) a intensive care unit (NICU) south Colombia. Study Design A nonmatched case–control was conducted. Convenience sampling performed. Data were obtained from clinical records. IL-6 levels determined using enzyme-linked immunosorbent assay. Receiver operator characteristic (ROC) curve analysis Youden's index used optimal cutoffs for CRP diagnosing NS,...
Bacterial meningitis (BM) is a pyogenic infection present in the subarachnoid space, potentially fatal and frequently associated with neurological sequelae. During BM, cytokines (CTs) are locally produced. We sought to determine CTs’ clinical role as disease severity predictors adults, which not completely clear. Using bead-based flow cytometric assay, levels of six CTs were determined cerebrospinal fluid (CSF) plasma from 18 adult BM patients 19 uninfected controls. Long-term sequelae...