A5000115796- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Biosensors and Analytical Detection
- COVID-19 epidemiological studies
- Malaria Research and Control
- Gold and Silver Nanoparticles Synthesis and Applications
- COVID-19 Clinical Research Studies
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- vaccines and immunoinformatics approaches
- Transgenic Plants and Applications
- Phytoplasmas and Hemiptera pathogens
- COVID-19 Impact on Reproduction
- Vector-Borne Animal Diseases
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- COVID-19 and healthcare impacts
- Virology and Viral Diseases
- Immunotherapy and Immune Responses
- Toxin Mechanisms and Immunotoxins
- COVID-19 and Mental Health
- Electrophoretic Deposition in Materials Science
- Nanoparticle-Based Drug Delivery
- T-cell and Retrovirus Studies
Centro de Desenvolvimento da Tecnologia Nuclear
2025
The University of Texas Medical Branch at Galveston
2022-2024
Faculdade de Medicina de São José do Rio Preto
2019-2023
Galveston College
2023
Universidade Federal de Minas Gerais
2014-2022
ATRP Solutions (United States)
2021
Massachusetts Institute of Technology
2017
Universidade Federal de Ouro Preto
2017
Universidade Federal de Viçosa
2015-2016
Although used in medical applications for centuries, the development of nanotechnology has shed new light plethora possible and biological using gold-based nanostructures. Gold nanostructures are stable relatively inert systems, leading to low reatogenicity, biocompatibility general lack toxicity. Allied that, gold nanoparticles present optical electronic properties that have been exploited a range biomedical applications. In this review we discuss biologically relevant how they some...
The emergence of the Brazilian variant concern, Gamma lineage (P.1), impacted epidemiological profile COVID-19 cases due to its higher transmissibility rate and immune evasion ability.
Abstract Background Cellular entry of SARS-CoV-2 has been shown to rely on angiotensin-converting enzyme 2 (ACE2) receptors, whose expression in the testis is among highest body. Additionally, risk mortality seems higher male COVID-19 patients, and though much published since first cases COVID-19, there remain unanswered questions regarding impact testes potential consequences for reproductive health. We investigated testicular alterations non-vaccinated deceased COVID-19-patients, precise...
Dengue is the most prevalent arthropod-borne viral disease in world. In this article we present results on development, characterization and immunogenic evaluation of an alternative vaccine candidate against Dengue. The MWNT-DENV3E nanoconjugate was developed by covalent functionalization carboxylated multi-walled carbon nanotubes (MWNT) with recombinant dengue envelope (DENV3E) proteins. antigens were bound to MWNT using a diimide-activated amidation process immunogen characterized TEM, AFM...
Background The co-circulation of flaviviruses in tropical regions has led to the hypothesis that immunity generated by a previous dengue infection could promote severe disease outcomes subsequent infections heterologous serotypes. This study investigated influence antibodies Zika on clinical infection. Methodology/Principal findings We enrolled 1,043 laboratory confirmed patients and their prior or dengue. Severe forms were more frequent with infection, but not those previously exposed...
Abstract The Flaviviridae virus family was named after the Yellow-fever , and latin term flavi means “of golden color”. Dengue, caused by Dengue (DENV), is one of most important infectious diseases worldwide. A sensitive differential diagnosis crucial for patient management, especially due to occurrence serological cross-reactivity other co-circulating flaviviruses. This became particularly with emergence Zika (ZIKV) in areas were DENV seroprevalence already high. We developed a specific...
Background Chikungunya virus (CHIKV) has spread across Brazil with varying incidence rates depending on the affected areas. Due to cocirculation of arboviruses and overlapping disease symptoms, CHIKV infection may be underdiagnosed. To understand lack epidemics in São José do Rio Preto (SJdRP), Paulo (SP), Brazil, we evaluated viral circulation by investigating anti-CHIKV IgG seroconversion a prospective study asymptomatic individuals detecting IgM suspected dengue infection, as well...
Surface enhanced Raman spectroscopy (SERS) can be used to enhance the sensitivity of lateral flow immunoassays.
Background Dengue virus (DENV) infections pose one of the largest global barriers to human health. The four serotypes (DENV 1–4) present different symptoms and influence immune response subsequent DENV infections, rendering surveillance, risk assessments, disease control particularly challenging. Early diagnosis appropriate clinical management is critical can be achieved by detecting nonstructural protein 1 (NS1) in serum during acute phase. However, few NS1-based tests have been developed...
Ilhéus virus (ILHV)(
ABSTRACT Although much has been published since the first cases of COVID-19, there remain unanswered questions regarding SARS-CoV-2 impact on testes and potential consequences for reproductive health. We investigated testicular alterations in deceased COVID-19-patients, precise location virus, its replicative activity, molecules involved pathogenesis. found that tropism is higher than previously thought reliable viral detection testis requires sensitive nanosensoring or RT-qPCR using a...
In recent times, studies have demonstrated that carbon nanotubes are good candidates for use as vehicles transfection of exogenous material into the cells. However, there few evaluating behavior DNA vectors and these used multi-walled (MWCNTs) or carboxylated MWCNTs. Thus, this study aims to assess MWCNTs' (carboxylated not) efficiency in increase expression tetravalent vaccine candidate (TVC) plasmid vector dengue virus vitro using Vero cells, vivo, through intramuscular route, evaluate...
Abstract High frequency screening of populations has been proposed as a strategy in facilitating control the COVID-19 pandemic. We use computational modeling, coupled with clinical data from rapid antigen tests, to predict impact frequent viral testing on spread and outcomes. Using patient nasal or nasopharyngeal swab specimens, we demonstrate that sensitivity/specificity two tests compared quantitative real-time polymerase chain reaction (qRT-PCR) are 82.0%/100% 84.7%/85.7%, respectively;...
Abstract High frequency screening of populations has been proposed as a strategy in facilitating control the COVID-19 pandemic. We use computational modeling, coupled with clinical data from rapid antigen tests, to predict impact frequent viral testing on spread and outcomes. Using patient nasal or nasopharyngeal swab specimens, we demonstrate that sensitivity/specificity two tests compared quantitative real-time polymerase chain reaction (qRT-PCR) are 80.0%/91.1% 84.7%/85.7%, respectively;...
Dengue is a serious mosquito-transmitted disease caused by the dengue virus (DENV). Rapid and reliable diagnosis of DENV infection urgently needed in dengue-endemic regions. We describe here performance evaluation CE-marked VIDAS® immunoassays developed for automated detection NS1 antigen anti-DENV IgM IgG antibodies. A multicenter concordance study was conducted 1296 patients from regions Asia, Latin America, Africa. results were compared to those competitor enzyme-linked immunosorbent...
Abstract In this paper, a localized surface plasmon resonance (LSPR)‐based biosensor, computationally guided to detect selectively the four Dengue virus (DENV x ) serotypes in seropositive patients, is presented. The behavior of gold nanoparticles shape nanorods theoretically and numerically studied as function induced structural variations, which are experimentally evidenced due bio‐interaction between target analytes its during detection process. Additionally, with implementation largest...
Introduction The present work sought to identify MHC-I-restricted peptide signatures for arbovirus using in silico and vitro microarray tools. Methods First, an in-silico analysis of immunogenic epitopes restricted four the most prevalent human MHC class-I was performed by identification affinity score. For that, more than 10,000 sequences from 5 Arbovirus 8 different viral serotypes, namely Zika (ZIKV), Dengue (DENV serotypes 1-4), Chikungunya (CHIKV), Mayaro (MAYV) Oropouche (OROV)...
Vaccination against Anaplasma marginale has been considered an important control strategy for bovine anaplasmosis. Recently, mice immunized with rMSP1 a linked to carbon nanotubes (MWNT) showed significant immune responses, generating new possibility use of inactivated vaccine. The objective this study was investigate the cellular and humoral responses in calves MWNT+rMSP1a , associated vaccine A. produced vitro, evaluate toxic effects MWNT on renal hepatic function. rMSP1a covalently MWNT....