A5086738149- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Malaria Research and Control
- Virology and Viral Diseases
- Immune Response and Inflammation
- Viral Infections and Outbreaks Research
- S100 Proteins and Annexins
- Inflammasome and immune disorders
- Long-Term Effects of COVID-19
- Neuroinflammation and Neurodegeneration Mechanisms
- interferon and immune responses
- COVID-19 Impact on Reproduction
- Animal Virus Infections Studies
- Parvovirus B19 Infection Studies
- Rheumatoid Arthritis Research and Therapies
- Ion Transport and Channel Regulation
- Insect symbiosis and bacterial influences
- Receptor Mechanisms and Signaling
- Influenza Virus Research Studies
- Vitamin C and Antioxidants Research
- Infectious Encephalopathies and Encephalitis
- Insect and Pesticide Research
- Gut microbiota and health
Universidade Federal de Minas Gerais
2016-2025
Morpho (United States)
2021-2025
Brigham and Women's Hospital
2024
The University of Texas Health Science Center at Houston
2023
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2022
Universidade Federal de Itajubá
2018
Singapore-MIT Alliance for Research and Technology
2014-2017
Universidade de Ribeirão Preto
2015
Institut des Sciences Biologiques
2010
University of Iowa
2009
Deposition of monosodium urate monohydrate (MSU) crystals in the joints promotes an intense inflammatory response and joint dysfunction. This study evaluated role NLRP3 inflammasome 5-lipoxygenase (5-LOX)-derived leukotriene B(4) (LTB(4) ) driving tissue inflammation hypernociception a murine model gout.Gout was induced by injecting MSU into mice. Wild-type mice deficient NLRP3, ASC, caspase 1, interleukin-1β (IL-1β), IL-1 receptor type I (IL-1RI), IL-18R, myeloid differentiation factor 88...
Abstract A safe and effective vaccine with long-term protection against SARS-CoV-2 variants of concern (VOCs) is a global health priority. Here, we develop lipid nanoparticles (LNPs) to provide delivery plasmid DNA (pDNA) show VOCs in female small animal models. Using library LNPs encapsulating unique barcoded (b-DNA), screen for b-DNA after intramuscular administration. The top-performing are further tested their capacity pDNA uptake antigen-presenting cells vitro. lead LNP used encapsulate...
The ability of an individual to sense pain is fundamental for its capacity adapt environment and avoid damage. sensation can be enhanced by acute or chronic inflammation. In the present study, we have investigated whether inflammatory pain, as measured hypernociceptive responses, was modified in absence microbiota. To this end, evaluated mechanical nociceptive responses induced a range stimuli germ-free conventional mice. Our experiments show that hypernociception carrageenan,...
Abstract Objective The chemokine receptors CXCR1 and CXCR2 play a role in mediating neutrophil recruitment neutrophil‐dependent injury several models of inflammation. We undertook this study to investigate the these adhesion, subsequent migration, hypernociception murine model monarticular antigen‐induced arthritis (AIA). Methods AIA was induced by administration antigen into knee joint previously immunized mice. Intravital microscopy studies were performed assess leukocyte rolling adhesion....
Highlights•Structure-guided affinity enhancement of a cross-reactive dengue antibody•mAb neutralizes all four serotypes with low level viral-enhancing activity•Antibody demonstrates in vivo ability to resolve symptoms severe infection•Crystal structure antibody-antigen validates the predicted designsSummaryDengue is most common vector-borne viral disease, causing nearly 400 million infections yearly. Currently there are no approved therapies. Antibody epitopes that elicit weak humoral...
Abstract Activation of the renin-angiotensin (Ang) system induces inflammation via interaction between Ang II and type 1 receptor on leukocytes. The relevance new arm renin-Ang system, namely Ang-converting enzyme-2/Ang-(1–7)/Mas receptor, for inflammatory responses is not known was investigated in this study. For purpose, two experimental models were used: Ag-induced arthritis (AIA) mice adjuvant-induced (AdIA) rats. Male C57BL/6 wild-type or Mas−/− subjected to AIA treated with Ang-(1–7),...
Yellow fever virus (YFV) is a member of the Flaviviridae family. In Brazil, yellow (YF) cases have increased dramatically in sylvatic areas neighboring urban zones last few years. Because high lethality rates associated with infection and absence any antiviral treatments, it essential to identify therapeutic options respond YFV outbreaks. Repurposing clinically approved drugs represents fastest alternative discover antivirals for public health emergencies. Other Flaviviruses, such as Zika...
The teratogenic mechanisms triggered by ZIKV are still obscure due to the lack of a suitable animal model. Here we present mouse model developmental disruption induced hematogenic infection. utilizes immunocompetent animals from wild-type FVB/NJ and C57BL/6J strains, providing better analogy human condition than approaches involving immunodeficient, genetically modified animals, or direct injection into brain. When injected via jugular vein blood pregnant females harboring conceptuses early...
Abstract In Africa, Old World Primates are involved in the maintenance of sylvatic circulation ZIKV. However, Brazil, hosts for cycle remain unknown. We hypothesized that free-living NHPs might play a role urban/periurban ZIKV dynamics, thus we undertook an NHP investigation two cities Brazil. identified ZIKV-positive and sequences obtained were phylogenetically related to American lineage Additionally, inoculated four C . penicillata with our results demonstrated marmosets had sustained...
Long-term sequelae of coronavirus disease (COVID)-19 are frequent and major concern. Severe acute respiratory syndrome 2 (SARS-CoV-2) infection affects the host gut microbiota, which is linked to severity in patients with COVID-19. Here, we report that microbiota post-COVID subjects had a remarkable predominance Enterobacteriaceae strains an antibiotic-resistant phenotype compared healthy controls. Additionally, short-chain fatty acid (SCFA) levels were reduced feces. Fecal transplantation...
Introduction: Immunotherapy has revolutionized cancer treatment by harnessing the immune system to enhance antitumor responses while minimizing off-target effects. Among promising cancer-specific therapies, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) attracted significant attention. Methods: Here, we developed an ionizable lipid nanoparticle (LNP) platform deliver TRAIL mRNA (LNP-TRAIL) directly microenvironment (TME) induce cell death. Our LNP-TRAIL was formulated via...
Inflammation is elicited by the host in response to microbes, and believed be essential for protection against infection. However, we have previously hypothesized that excessive or misplaced inflammation may a major contributor tissue dysfunction death associated with viral bacterial infections. The resolutive phase of necessary condition achieve homeostasis after acute inflammation. It possible targeting resolution beneficial during In this review, summarize evidence demonstrating...
Rheumatoid arthritis (RA) and periodontal disease (PD) are prevalent chronic inflammatory disorders that affect bone structures. Individuals with RA more likely to experience PD, but how in joints could induce PD remains unknown. This study aimed experimentally mimic clinical parameters of RA-induced provide mechanistic findings explain this association. Chronic Ag-induced (AIA) was triggered by injection methylated BSA the knee joint immunized mice. Anti-TNF-α used assess role cytokine....
ABSTRACT Zika virus (ZIKV) infection is a global health emergency that causes significant neurodegeneration. Neurodegenerative processes may be exacerbated by N -methyl- d -aspartate receptor (NMDAR)-dependent neuronal excitoxicity. Here, we have exploited the hypothesis ZIKV-induced neurodegeneration can rescued blocking NMDA overstimulation with memantine. Our results show ZIKV actively replicates in primary neurons and replication directly associated massive cell death. Interestingly,...
Zika virus (ZIKV) has recently caused a worldwide outbreak of infections associated with severe neurological complications, including microcephaly in infants born from infected mothers. ZIKV exhibits high neurotropism and promotes neuroinflammation neuronal cell death. We have demonstrated that N-methyl-d-aspartate receptor (NMDAR) blockade by memantine prevents ZIKV-induced Here, we show induces apoptosis non-cell autonomous manner, triggering death uninfected neurons releasing cytotoxic...
Dengue is a mosquito-borne disease caused by one of four serotypes virus (DENV-1-4). Severe dengue infection in humans characterized thrombocytopenia, increased vascular permeability, hemorrhage and shock. However, there little information about host response to DENV infection. Here, mechanisms accounting for IFN-γ production effector function during were investigated murine model DENV-2 expression was greatly after mice its preceded increase IL-12 IL-18 levels. In IFN-γ(-/-) mice,...
Gout manifests as recurrent episodes of acute joint inflammation and pain due to the deposition monosodium urate (MSU) crystals within affected tissue in a process dependent on NLRP3 inflammasome activation. The synthesis, activation, release IL-1β are crucial for MSU-induced inflammation. current study evaluated mechanism by which TNF-α contributed Male C57BL/6J or transgenic mice were used this was induced injection MSU into joint. markedly increased after MSU. There inhibition...
The emergence of life-threatening zoonotic diseases caused by betacoronaviruses, including the ongoing coronavirus disease 19 (COVID-19) pandemic, has highlighted need for developing preclinical models mirroring respiratory and systemic pathophysiological manifestations seen in infected humans. Here, we showed that C57BL/6J wild-type mice intranasally inoculated with murine betacoronavirus hepatitis 3 (MHV-3) develop a robust inflammatory response leading to acute lung injuries, alveolar...