A5069083707- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced biosensing and bioanalysis techniques
- Polymer Surface Interaction Studies
- Plasmonic and Surface Plasmon Research
- Lipid Membrane Structure and Behavior
- Biosensors and Analytical Detection
- Force Microscopy Techniques and Applications
- Protein purification and stability
- Microfluidic and Bio-sensing Technologies
- Electrochemical Analysis and Applications
- Pickering emulsions and particle stabilization
- Advanced Biosensing Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Analytical Chemistry and Sensors
- Nanofabrication and Lithography Techniques
- Nanomaterials and Printing Technologies
- Luminescence Properties of Advanced Materials
- Monoclonal and Polyclonal Antibodies Research
- Acoustic Wave Resonator Technologies
- RNA Interference and Gene Delivery
- Nanocluster Synthesis and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Protein Interaction Studies and Fluorescence Analysis
- Proteins in Food Systems
- Perovskite Materials and Applications
Nanyang Technological University
2016-2025
Institute of Microelectronics
2008
Agency for Science, Technology and Research
2008
Singapore Science Park
2008
The common drawbacks of current colorimetric sensors using gold nanoparticle aggregation is its relatively low sensitivity and narrow dynamic range, which restrict their application in real sample analysis when competing with other analytical techniques such as fluorescence chemiluminescence. In this article, we demonstrate a novel strategy to construct based on aggregation. Unlike the conventional cause formation large aggregates, our strategy, dimers are selectively formed upon target...
We report the synthesis of uniform triangular gold nanoplates by a modified seeded growth method. The concentration cetyltrimethylammonium bromide (CTAB) in solution and time interval between multiple steps were important factors which determined formation Au nanoplates. In addition, further isotropic overgrowth, thickness can be finely tuned within wide range 10-80 nm, at present remains challenge using conventional growth.
Bovine serum albumin (BSA) is the most widely used protein for surface passivation applications, although it has relatively weak, nonsticky interactions with hydrophilic surfaces such as silica-based materials. Herein, we report a simple and versatile method to increase stickiness of BSA molecules adsorbing onto silica surfaces, resulting in up 10-fold improvement blocking efficiency against biofouling. Circular dichroism spectroscopy, dynamic light scattering, nanoparticle tracking analysis...
Abstract Lipid‐based nanoparticles have emerged as a clinically viable platform technology to deliver nucleic acids for wide range of healthcare applications. Within this scope, one the most exciting areas recent progress and future innovation potential lies in material science lipid‐based nanoparticles, both refine existing nanoparticle strategies develop new ones. Herein, latest efforts next‐generation are covered by taking nanoarchitectonics perspective design, acid encapsulation methods,...
We introduce a novel solid-phase colorimetric sensor facilely fabricated by loading unmodified gold nanoparticles into poly(oligo(ethylene glycol)methacrylate) (POEGMA) brushes grown on glass. Our work reports the first synergistic combination of metallic acting as sensing module with nonfouling polymer matrix both nonrigid scaffold and screen to reduce interference from nontarget molecules. In addition, nanocomposite is formed transparent substrate, detection can be performed in same manner...
Understanding the physicochemical factors that influence protein adsorption onto solid supports holds wide relevance for fundamental insights into structure and function as well applications such surface passivation.
Abstract Bovine serum albumin (BSA) protein is widely used to fabricate antifouling coatings for nanobiotechnology applications. Numerous BSA options are commercially available and obtained through different purification methods, however, there no guidance on which ones preferable coatings. Herein, we investigate the real-time fabrication of composed proteins report significant differences in ultrathin film coating properties ability mitigate biofouling prevent nanoparticle-induced immune...
The escalating global demand for sustainable manufacturing, motivated by concerns over energy conservation and carbon footprints, encounters challenges due to insufficient renewable materials arduous fabrication procedures fulfill specific requirements in medical healthcare systems. Here, biosafe pollen cryogel is engineered as effective hemostats without additional harmful crosslinkers treat deep noncompressible wounds. A straightforward low-energy approach involved forming stable...
Here, we present a mean to expand the use of individual metallic nanoparticles two-dimensional plasmonic nanoarrays. An optical detection platform track down localized surface plasmon resonance (LSPR) signals on substrates was built for application A pseudoimage substrate reconstructed from their scattering spectra obtained by scanning user-defined area. The spectral and spatial resolutions system were also discussed in detail. Most importantly, method normalize geometrically different...
Abstract The first reconfigurable colorimetric DNA switches based on target binding are reported. This actuates a change in the interparticle distance between gold nanoparticle dimers. A significant spectral shift of 68 nm is achievable from on‐off switching. reconfigurability possible owing to thiol and EDC‐imidazole coupling which anchors linkers nanoparticles. huge allows unaided eye observe single biomolecular event real time under darkfield microscope. limit‐of‐detection for molecules...
With mounting evidence that nanomaterials can trigger adverse innate immune responses such as complement activation, there is increasing attention to the development of strategies mask complement-activating properties nanomaterials. The current gold standard reduce activation covalent attachment polymer coatings on nanomaterial surfaces, even though this strategy provides only moderate protection against activation. Akin protein coronas form surfaces in physiological fluids, noncovalent...
Characterizing the shape of sub-100 nm, biological soft-matter particulates (e.g., liposomes and exosomes) adsorbed at a solid-liquid interface remains challenging task. Here, we introduce localized surface plasmon resonance (LSPR) sensing approach to quantitatively profile deformation nanoscale, fluid-phase 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) contacting titanium dioxide substrate. Experimental theoretical results validate that, due its high sensitivity spatial proximity...
The combination of label-free, surface-sensitive measurement techniques based on different physical principles enables detailed characterization biomacromolecular interactions at solid-liquid interfaces. To date, most combined systems have involved experimental with similar probing volumes, whereas the potential utilizing surface sensitivities remains largely unexplored, especially for data interpretation. Herein, we report a approach that integrates conventional quartz crystal...
Silica-coated nanoparticles are widely used in biomedical applications such as theranostics, imaging, and drug delivery. While silica-coated biocompatible, experimental evidence shows that they can trigger innate immune reactions, a broader understanding of what types reactions caused how to mitigate them is needed. Herein, we investigated the noncovalent surface functionalization silica with purified proteins inhibit nanoparticle-induced complement activation macrophage uptake, two most...
The effect of ionic strength as well surfactant concentration on the surface assembly cetyltrimethylammonium bromide (CTAB)-capped gold nanorods (GNRs) has been studied. Glass substrates were modified to yield a net negative charge through electrostatic coating polystyrenesulfonate (PSS) over self-assembled monolayer (SAM) positively charged aminopropyltriethoxysilane (APTS). then fully immersed in GNR solutions at different CTAB concentrations and strengths. Under slightly excess...
A novel permeability gate-based electrochemiluminescent (ECL) aptasensor has been constructed by utilizing target-responsive polyelectrolyte–aptamer film deposited on the solid-state ECL electrode to control rate of diffusion a coreactant that triggers ECL.
Abstract The design and application of surface-based nanoplasmonic sensors has spurred broad interest from the chemical science community, touching upon diverse topics such as plasmonics, nanoscience, surface chemistry, measurement analysis, interfacial science. One most exciting areas involves taking advantage simple instrumental requirements high sensitivity these sensing devices to study biomacromolecules biological nanoparticles. In this Account, we present a narrative summary describing...
Understanding the adsorption behavior of lipid vesicles at solid-liquid interfaces is important for obtaining fundamental insights into soft matter adsorbates as well practical applications such supported bilayer (SLB) fabrication. While process SLB formation has been highly scrutinized, less understood are details vesicle without rupture, especially high surface coverages. Herein, we tackle this problem by employing simultaneous quartz crystal microbalance-dissipation (QCM-D) and localized...