A5025878801- RNA and protein synthesis mechanisms
- Zoonotic diseases and public health
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- RNA modifications and cancer
- COVID-19 Clinical Research Studies
- Polyamine Metabolism and Applications
- Infectious Encephalopathies and Encephalitis
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Mitochondrial Function and Pathology
- Influenza Virus Research Studies
- Autophagy in Disease and Therapy
- Mast cells and histamine
- CRISPR and Genetic Engineering
- Cancer Immunotherapy and Biomarkers
- Metabolism, Diabetes, and Cancer
- PI3K/AKT/mTOR signaling in cancer
Flinders University
2023-2024
The University of Adelaide
2019-2021
South Australian Health and Medical Research Institute
2017-2021
Eukaryotic elongation factor 2 kinase (eEF2K) negatively regulates the phase of mRNA translation and hence protein synthesis. Increasing evidence indicates that eEF2K plays an important role in survival migration cancer cells tumor progression. As demonstrated by two‐dimensional wound‐healing three‐dimensional transwell invasion assays, knocking down or inhibiting impairs cells. Conversely, exogenous expression eEF2 (the substrate eEF2K) accelerates wound healing invasion. Importantly, using...
Emerging advances in cancer therapy have transformed the landscape towards immunotherapy regimens. Recent discoveries resulted development of clinical immune checkpoint inhibitors that are 'game-changers' for immunotherapy. Here we show eEF2K, an atypical protein kinase negatively modulates elongation stage synthesis, promotes synthesis PD-L1, which helps cells to escape from immunosurveillance. Ablation eEF2K prostate and lung markedly reduced expression levels PD-L1 protein. We association...
The rat is a useful laboratory model for respiratory disease, and SARS-CoV-2 proteins such as the spike (S) protein can induce inflammation. This study has investigated ability of Q498Y, P499T (QP-YT) amino acid change described in S-protein mouse adapted MA strain, to interact with angiotensin converting enzyme-2 (ACE2) stimulate responses lung. Using real-time S-ACE2 quantitative fusion assay, ancestral fuses human, but not ACE2. QP-YT retains fuse human ACE2, interacts ACE2 assay using...
The rat is a useful laboratory model for respiratory disease, and SARS-CoV-2 proteins such as the spike (S) protein can induce inflammation. This study has investigated ability of Q498Y, P499T (QP-YT) amino acid change described in S-protein mouse adapted MA strain, to interact with angiotensin converting enzyme-2 (ACE2) stimulate responses lung. Using real-time S-ACE2 quantitative fusion assay, ancestral L452R fuses human, but not ACE2 expressed on HEK293 cells. QP-YT retains fuse human...
The rat is a useful laboratory model for respiratory diseases. SARS-CoV-2 proteins, such as the spike (S) protein, can induce inflammation. This study has investigated ability of Q498Y, P499T (QP-YT) amino acid change, described in S-protein mouse-adapted MA strain, to interact with angiotensin converting enzyme-2 (ACE2) and stimulate responses lungs. A real-time S–ACE2 quantitative fusion assay shows that ancestral L452R S-proteins fuse human but not ACE2 expressed on HEK293 (human...
Cells within solid tumours can become deprived of nutrients; in order to survive, they need invoke mechanisms conserve these resources. Using cancer cells culture the absence key nutrients, we have explored roles two potential survival mechanisms, autophagy and elongation factor 2 kinase (eEF2K), which, when activated, inhibits resource-intensive stage protein synthesis. Both processes are regulated through nutrient-sensitive AMP-activated mechanistic target rapamycin complex 1 signalling...
Abstract Emerging advances in cancer therapy have transformed the landscape from conventional therapies towards immunotherapy regimens. Recent discoveries resulted development of clinical immune checkpoint inhibitors that are ‘game-changers’ for immunotherapy. Here we show eEF2K, an atypical protein kinase inhibits elongation stage synthesis, actually promotes synthesis PD-L1, which helps cells to escape immunosurveillance. Ablation eEF2K prostate and lung markedly reduced expression levels...