A5100448676- CRISPR and Genetic Engineering
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Advanced materials and composites
- Immunotherapy and Immune Responses
- Bacterial Infections and Vaccines
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Advanced Mathematical Modeling in Engineering
- Calcium signaling and nucleotide metabolism
- Virus-based gene therapy research
- Extracellular vesicles in disease
- Plant Surface Properties and Treatments
- Advanced Antenna and Metasurface Technologies
- Microtubule and mitosis dynamics
- Advanced MEMS and NEMS Technologies
- Mosquito-borne diseases and control
- Cholesterol and Lipid Metabolism
- Insect symbiosis and bacterial influences
- Laser Applications in Dentistry and Medicine
- Genetics, Aging, and Longevity in Model Organisms
- Genetics, Bioinformatics, and Biomedical Research
Shanghai Jiao Tong University
2006-2024
Nanjing Normal University
2021-2024
Chengdu University
2024
State Key Laboratory of Oncogene and Related Genes
2021-2023
Renji Hospital
2021-2023
Shanghai Cancer Institute
2022-2023
Henan Agricultural University
2023
Southern Medical University
2022
Qiqihar Medical University
2022
Nanchang Hangkong University
2022
Despite bacterial-mediated biotherapies have been widely explored for treating different types of cancer, their implementation has restricted by low treatment efficacy, due largely to the absence tumor-specific accumulation following administration. Here, conjugation aptamers bacterial surface is described a simple and cytocompatible amidation procedure, which can significantly promote localization bacteria in tumor site after systemic The density be easily adjusted varying feed ratio able...
Outer membrane vesicles (OMVs) play vital roles in bacterial communication both intraspecifically and interspecifically. However, extracellular mechanisms of gut microbiota-derived OMVs the intestine remain poorly understood. Here, we report that released from Akkermansia muciniphila are able to (i) restore disturbed balance microbiota by selectively promoting proliferation beneficial bacteria through fusion, (ii) elicit mucosal immunoglobulin A response translocating into Peyer's patches...
Abstract The power of synthetic biology has enabled the expression heterologous pathways in cells, as well genome-scale synthesis projects. complexity biological networks makes rational de novo design a grand challenge. Introducing features that confer genetic flexibility is powerful strategy for downstream engineering. Here we develop an vitro method DNA library construction based on structural variation to accomplish this goal. “in SCRaMbLE system” uses Cre recombinase mixed test tube with...
Chirality is ubiquitous in biological systems, which closely related to functions, life processes, and even the pathogenesis of diseases. However, interface between chirality synthetic materials organisms, particularly immune system, remains poorly understood. Here, supramolecular chiral polymer micelles (SCPMs) are prepared by complexing antigenic proteins with amino acid-modified polyethyleneimine. The introduction not only reduces toxicity cationic polymer, but also benefits cell uptake...
Abstract High-quality development plays a crucial role in China’s economic progress the new era. It represents concept of advancement and mirrors increasing aspirations populace for an improved standard living. In this context, artificial intelligence (AI) promoting sustainable cannot be overemphasized. This paper explores how AI technologies can drive transition to green, low-carbon circular economy. We have established index system measure level industry high-quality economy, which is...
Outer membrane vesicles (OMVs) are crucial for bacterial intercellular communication and the crosstalk between gut microbiota its host. Methods capable of visualizing derived OMVs would be great significance but have been rarely reported. Here, nanoprobes carrying a fluorescence-activating absorption-shifting tag prepared by combining genetic engineering antibiotic-boosted vesicle formation release. Benefiting from their natural structure molecular oxygen-independent emission, resulting...
Autophagy is a cellular catabolic process by which long-lived proteins and damaged organelles are degradated lysosomes. Activation of autophagy an important survival mechanism that protects cancer cells from various stresses, including anticancer agents. Recent studies indicate pyrvinium pamoate, FDA-approved antihelminthic drug, exhibits wide-ranging activity. Here we demonstrate inhibits both in vitro vivo. We further the inhibition mammalian target rapamycin independent but depends on...
Ribosome biogenesis is a key process for maintaining protein synthetic capacity in dividing or growing cells, and requires coordinated production of ribosomal proteins RNA (rRNA), including the processing latter. Signalling through mammalian target rapamycin complex 1 (mTORC1) activates all these processes. Here, we show that, human impaired rRNA processing, caused by expressing an interfering mutant BOP1 knocking down components PeBoW elicits activation mTORC1 signalling. This leads to...
Abstract Chloroplasts are major photosynthetic and protein-containing organelles in green plants algae. Unwanted chloroplast proteins entire chloroplasts cleared through various degradation pathways including autophagy. Nevertheless, canonical chlorophagy receptors remain unidentified, whether to what extent can be enhanced benefect the unknown. Here we designed validated a synthetic autophagy receptor using biochemical, genetical, imaging approaches. This receptor, LIR-SNT-BFP, was...
The typeⅡCRISPR/Cas9 system (Clustered regularly interspaced short palindromic repeats /CRISPR-associated 9) has been widely used in bacteria, yeast, animals and plants as a targeted genome editing technique. In previous work, we have successfully knocked out the endogenous phytoene dehydrogenase (PDS) gene Populus tomentosa Carr. using this system. To study effect of target design on efficiency CRISPR/Cas9-mediated knockout Populus, analyzed mutagenesis different single-guide RNA (sgRNA)...
Vibrio anguillarum is a gram-negative halophilic bacterium that causes vibriosis in marine fish, freshwater fish and other aquatic animals. Bacteria have developed strategies to survive harsh environments. The alternative sigma factor, RpoS (sigma(S)), plays key role surviving under stress conditions some bacteria. An rpoS mutant of pathogenic V. W-1 was constructed by homologous recombination. sensitivity the osmotic [2.4 M NaCl artificial seawater (ASW)] did not change obviously, but high...
Melanoma is the most dangerous skin cancer due to its highly metastatic potential and resistance chemotherapy. Currently, there no effective treatment for melanoma once it progressed stage. Therefore, further study elucidate molecular mechanism underlying metastasis of cells urgently required improvement treatment. In present study, we found that diphthamide synthesis 3 (Dph3) involved in B16F10 murine by insertional mutagenesis. We demonstrated Dph3 disruption impairs migration cells. The...
The cooking and eating quality of rice grains is a major focus from consumer’s perspective mainly determined by the apparent amylose content (AAC) starch. Waxy rice, type with an AAC less than 2%, important goal for breeding high-quality rice. In recent years, cloning ( Wx) gene has revealed molecular mechanism formation waxy traits in However, there have been limited studies on physicochemical properties, such as gelatinization temperature, rapid viscosity analyzer profile, amylopectin fine...
Abstract Reactive oxygen species (ROS)‐induced oxidative DNA damages have been considered the main cause of mutations in genes, which are highly related to carcinogenesis and tumour progression. Extracellular vesicles play an important role cancer metastasis. However, precise damage extracellular (EVs)‐mediated cell migration invasion remains unclear. Here, we reveal that ROS‐mediated signalling promotes metastasis through increasing EVs release. Mechanistically, 8‐oxoguanine glycosylase...
Ulmus mianzhuensis is an endemic tree species in China with high ornamental and economic value. Currently, little known regarding its genomic architecture, phylogenetic position, or adaptive evolution. Here, we sequenced the complete chloroplast genome (cp genome) of U. further compared variations gene organization structure within to define their evolution, then reconstructed phylogenomic relationship 31 related explore systematic position utility cp for resolving phylogenetics among species.
Autophagy is a conserved intracellular trafficking pathway for bulk degradation and recycling of cellular components in eukaryotes. The hallmark autophagy the formation double-membraned vesicles termed autophagosomes, which selectively or non-selectively pack up various macromolecules organelles deliver these cargoes into vacuole/lysosome. Like all other membrane pathways, observation largely dependent on marker lines. ATG8/LC3 only autophagy-related (ATG) protein that, through covalent bond...