A5004038425- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Analytical Chemistry and Chromatography
- Food Allergy and Anaphylaxis Research
- Celiac Disease Research and Management
- Pharmacogenetics and Drug Metabolism
- Cancer, Lipids, and Metabolism
- Alzheimer's disease research and treatments
- Allergic Rhinitis and Sensitization
- Cancer therapeutics and mechanisms
- Cholesterol and Lipid Metabolism
- Probiotics and Fermented Foods
- Cancer, Hypoxia, and Metabolism
- Bioinformatics and Genomic Networks
- Advanced Chemical Sensor Technologies
- Drug Transport and Resistance Mechanisms
- Extracellular vesicles in disease
- Renal Diseases and Glomerulopathies
- Microbial Metabolic Engineering and Bioproduction
- Lipid metabolism and biosynthesis
- Coffee research and impacts
- Advanced Nanomaterials in Catalysis
- Congenital heart defects research
- Contact Dermatitis and Allergies
University of Surrey
2022-2025
Waters (United Kingdom)
2014-2024
University of Manchester
2020-2024
Waters (United States)
2013-2024
Manchester Academic Health Science Centre
2022
University of Milan
2021
University of Aberdeen
2012
The toxicity of metal oxide nanomaterials and their antimicrobial activity is attracting increasing attention. Among these materials, MgO particularly interesting as a low cost, environmentally‐friendly material. MgO, similar to other nanomaterials, commonly attributed the production reactive oxygen species (ROS). We investigated three different nanoparticle samples, clearly demonstrated robust towards Escherichia coli bacterial cells in absence ROS for two samples. Proteomics data also...
Abstract We performed a comprehensive investigation of the toxicity ZnO and TiO 2 nanoparticles using Escherichia coli as model organism. Both materials are wide band gap n-type semiconductors they can interact with lipopolysaccharide molecules present in outer membrane E. , well produce reactive oxygen species (ROS) under UV illumination. Despite similarities their properties, response bacteria to two nanomaterials was fundamentally different. When ROS generation is observed, nanomaterial...
As large scale metabolic phenotyping is increasingly employed in preclinical studies and the investigation of human health disease current LC-MS/MS profiling methodologies adopted for sample sets can result lengthy analysis times, putting strain on available resources. a these pressures rapid methods untargeted may have value where numbers samples require screening.To develop, characterise evaluate UHP-HILIC-MS-based method polar metabolites rat urine then extend capabilities this approach...
In the last decade, a revolution in liquid chromatography-mass spectrometry (LC-MS) based proteomics was unfolded with introduction of dozens novel instruments that incorporate additional data dimensions through innovative acquisition methodologies, turn inspiring specialized analysis pipelines. Simultaneously, growing number datasets have been made publicly available repositories such as ProteomeXchange, Zenodo and Skyline Panorama. However, developing algorithms to mine this assessing...
The unique physiochemical properties of wheat gluten enable a diverse range food products to be manufactured. However, triggers coeliac disease, condition which is treated using gluten-free diet. Analytical methods are required confirm if foods gluten-free, but current immunoassay-based can unreliable and proteomic offer an alternative require comprehensive well annotated sequence databases lacking for gluten. A manually curated database (GluPro V1.0) proteins, comprising 630 discrete full...
Abstract Extracellular vesicles (EVs) are emerging in tissue engineering as promising acellular tools, circumventing many of the limitations associated with cell‐based therapies. Epigenetic regulation through histone deacetylase (HDAC) inhibition has been shown to increase differentiation capacity. Therefore, this study aimed investigate potential augmenting osteoblast epigenetic functionality using HDAC inhibitor Trichostatin A (TSA) enhance therapeutic efficacy osteoblast‐derived EVs for...
There has been increasing interest in bacterial lipids recent years due, part, to their emerging role as molecular signalling molecules. Bacteroides thetaiotaomicron is an important member of the mammalian gut microbiota that shown produce sphingolipids (SP) pass through epithelial barrier impact host SP metabolism and signal into inflammation pathways. B. also produces a novel family N-acyl amines (called glycine lipids) are potent ligands Toll-like receptor 2 (TLR2). Here, we specifically...
Profiling allergens in complex food ingredients used oral challenges and immunotherapy is crucial for regulatory acceptance. Mass spectrometry based analysis employing data-independent acquisition coupled with ion mobility mass spectrometry-mass (DIA-IM-MS) was to investigate the allergen composition of raw peanuts roasted peanut flour challenge meals. This comprehensive qualitative quantitative using label-free approaches identified quantified 123 unique protein accessions. Semiquantitative...
Amino acids (AAs) and their metabolites are important building blocks, energy sources, signaling molecules associated with various pathological phenotypes. The quantification of AA tryptophan (TRP) in human serum plasma is therefore great diagnostic interest. Therefore, robust, reproducible sample extraction processing workflows as well rapid, sensitive absolute required to identify candidate biomarkers improve screening methods. We developed a validated semi-automated robotic liquid...
Abstract Time-of-day variation in the molecular profile of biofluids and tissues is a well-described phenomenon, but—especially for proteomics—is rarely considered terms challenges this presents to reproducible biomarker identification. We provide case study analysis human circadian ultradian rhythmicity proteins, including complement coagulation cascades apolipoproteins, with PLG, CFAH, ZA2G ITIH2 demonstrated as rhythmic first time. also show that increases risk Type II errors due...
Wheat is the most important food crop in world, unique physiochemical properties of wheat gluten enabling a diverse range products to be manufactured. However, genetic and environmental factors affect technological unpredictable ways. Although newer proteomic methods have potential offer much greater levels information, it older gel-based that remain commonly used identify compositional differences responsible for variation functionality, part due nature their primary sequences. A...
Obesity and dyslipidaemia are features of the metabolic syndrome risk factors for chronic kidney disease. The cellular mechanisms connecting with disease onset progression remain largely unclear. We show that proximal tubular epithelium is a target site lipid deposition upon overnutrition cholesterol-rich Western-type diet. Affected tubule epithelial cells displayed giant vacuoles lysosomal or autophagosomal origin, harbouring oxidised lipoproteins concentric membrane layer structures...
Sexual differences have been observed in the onset and prognosis of human cardiovascular diseases, but underlying mechanisms are not clear. Here, we found that zebrafish heart regeneration is faster females, can be accelerated by estrogen suppressed estrogen-antagonist tamoxifen. Injuries to heart, other tissues, increased plasma levels expression receptors, especially esr2a. The resulting endocrine disruption induces female-specific protein vitellogenin male zebrafish. Transcriptomic...
Preterm birth (PTB) is defined as occurring before 37 weeks' gestation, affects 5-9% of all pregnancies in developed countries, and the leading cause perinatal mortality. Spontaneous preterm (sPTB) accounts for 31-50% PTB, but underlying pathophysiology poorly understood.This study aimed to decipher lipidomics pathways involved sPTB.Blood samples were taken from SCreening fOr Pregnancy Endpoints (SCOPE), an international that recruited 5628 nulliparous women, with a singleton low-risk...
The use of gas-phase ion mobility separation in LC-MS based bottom-up proteomic experiments is described. A data-independent acquisition approach was employed combination with one and two dimensional reversed phase liquid chromatography separations. component the schema affords additional specificity improved peptide resolution terms system peak capacity. mainly achieved by means combined retention drift time association product to precursor ions chemical noise reduction. Experimental data...
Eukaryotic elongation factor 2 kinase (eEF2K) negatively regulates the phase of mRNA translation and hence protein synthesis. Increasing evidence indicates that eEF2K plays an important role in survival migration cancer cells tumor progression. As demonstrated by two‐dimensional wound‐healing three‐dimensional transwell invasion assays, knocking down or inhibiting impairs cells. Conversely, exogenous expression eEF2 (the substrate eEF2K) accelerates wound healing invasion. Importantly, using...
Peanut is an important food allergen, but it cannot currently be reliably detected and quantified in processed foods at low levels. A level of 3 mg protein/kg increasingly being used as a reference dose above which precautionary allergen labeling applied to products. Two exemplar matrices (chocolate dessert chocolate bar) were prepared incurred with 0, 3, 10, or 50 mg/kg peanut protein using commercially available lightly roasted flour ingredient. After simple buffer extraction employing...
Prostate cancer is the most common malignant tumour in men. Improved testing for diagnosis, risk prediction, and response to treatment would improve care. Here, we identified a proteomic signature of prostate peripheral blood using data-independent acquisition mass spectrometry combined with machine learning. A highly predictive was derived, which associated relevant pathways, including coagulation, complement, clotting cascades, as well plasma lipoprotein particle remodeling. We further...
Identification of features with high levels confidence in liquid chromatography–mass spectrometry (LC–MS) lipidomics research is an essential part biomarker discovery, but existing software platforms can give inconsistent results, even from identical spectral data. This poses a clear challenge for reproducibility identification. In this work, we illustrate the gap two open-access platforms, MS DIAL and Lipostar, finding just 14.0% identification agreement when analyzing LC–MS spectra using...
Wheat gluten, and related prolamin proteins in rye, barley oats cause the immune-mediated gluten intolerance syndrome, coeliac disease. Foods labelled as gluten-free which can be safely consumed by patients, must not contain above a level of 20mg/Kg. Current immunoassay methods for detection give conflicting results may underestimate levels foods. Mass spectrometry have great potential an orthogonal method, but require curated protein sequence databases to support method development. The...
There is an unmet need for improved diagnostic testing and risk prediction cases of prostate cancer (PCa) to improve care reduce overtreatment indolent disease. Here we have analysed the serum proteome lipidome 262 study participants by liquid chromatography-mass spectrometry, including diagnosed with PCa, benign prostatic hyperplasia (BPH), or otherwise healthy volunteers, aim improving biomarker specificity. Although a two-class machine learning model separated PCa from controls...