A5042331837- Advanced Proteomics Techniques and Applications
- Bone Tissue Engineering Materials
- Liver Disease Diagnosis and Treatment
- Extracellular vesicles in disease
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Mass Spectrometry Techniques and Applications
- Ocular Surface and Contact Lens
- Glycosylation and Glycoproteins Research
- Autophagy in Disease and Therapy
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Mycobacterium research and diagnosis
- Reproductive System and Pregnancy
- Glaucoma and retinal disorders
- Cancer-related molecular mechanisms research
- Galectins and Cancer Biology
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Ferroptosis and cancer prognosis
- Liver physiology and pathology
- Inflammatory Bowel Disease
CIC bioGUNE
2016-2025
Centro de Investigación Biomédica en Red
2016-2025
Instituto de Salud Carlos III
2016-2025
Euskadiko Parke Teknologikoa
2016-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2015-2025
Boeing (Spain)
2023
Digital Research Alliance of Canada
2021-2023
Association of Electronic and Information Technologies
2023
Hospital de Basurto
2021
Ikerbasque
2021
Exosomes represent a discrete population of vesicles that are secreted from various cell types to the extracellular media. Their protein and lipid composition consequence sorting events at level multivesicular body, central organelle which integrates endocytic secretory pathways. Characterization exosomes different biological samples has shown presence common as well cell-type specific proteins. Remarkably, content is modified upon pathological or stress conditions. Hepatocytes play role in...
Characterization of glycoproteins using mass spectrometry ranges from determination carbohydrate-protein linkages to the full characterization all glycan structures attached each glycosylation site. In a novel approach identify N-glycosylation sites in complex biological samples, we performed an enrichment glycosylated peptides through hydrophilic interaction liquid chromatography (HILIC) followed by partial deglycosylation combination endo-beta-N-acetylglucosaminidases (EC 3.2.1.96). After...
Although Schwann cell myelin breakdown is the universal outcome of a remarkably wide range conditions that cause disease or injury to peripheral nerves, cellular and molecular mechanisms make cell–mediated digestion possible have not been established. We report cells degrade after by novel form selective autophagy, myelinophagy. Autophagy was up-regulated myelinating nerve injury, debris present in autophagosomes, pharmacological genetic inhibition autophagy impaired clearance. Myelinophagy...
Cholangiocarcinoma (CCA) includes a heterogeneous group of biliary cancers with poor prognosis. Several conditions, such as primary sclerosing cholangitis (PSC), are risk factors. Noninvasive differential diagnosis between intrahepatic CCA and hepatocellular carcinoma (HCC) is sometimes difficult. Accurate noninvasive biomarkers for PSC, CCA, HCC not available. In the search novel biomarkers, serum extracellular vesicles (EV) were isolated from (n = 43), PSC 30), or 29) patients healthy...
Abstract Newly growing evidence highlights the essential role that epitranscriptomic marks play in development of many cancers; however, little is known about and implications altered epitranscriptome deposition prostate cancer. Here, we show transfer RNA N 7 -methylguanosine (m G) transferase METTL1 highly expressed primary advanced tumours. Mechanistically, find depletion causes loss m G tRNA methylation promotes biogenesis a novel class small non-coding RNAs derived from 5'tRNA fragments....
There is a compelling clinical imperative to identify discerning molecular biomarkers of hepatic disease in order inform the diagnosis, prognosis and treatment.We have investigated proteome urinary vesicles present urine samples obtained from experimental models for study liver injury, as an approach identifying potential disease.The biochemical proteomic characterization highly purified exosome-like has identified 28 proteins previously unreported these vesicles, many that been associated...
Nonalcoholic steatohepatitis (NASH) is the advanced form of nonalcoholic fatty liver disease (NAFLD) which sets stage for further damage. The mechanism progression NASH involves multiple parallel hits including oxidative stress, mitochondrial dysfunction, inflammation and others. Manipulation any these pathways may be an approach to prevent development progression. Aramchol (arachidyl-amido cholanoic acid) presently in a phase IIb study. aim this study was investigate Aramchol's action its...
Acetaminophen (APAP) is the active component of many medications used to treat pain and fever worldwide. Its overuse provokes liver injury it second most common cause failure. Mitochondrial dysfunction contributes APAP-induced but mechanism by which APAP causes hepatocyte toxicity not completely understood. Therefore, we lack efficient therapeutic strategies this pathology. Here show that interferes with formation mitochondrial respiratory supercomplexes via negative regulator MCJ, leads...
Objective Hepatocellular carcinoma (HCC) is a prevalent and aggressive cancer usually arising on background of chronic liver injury involving inflammatory hepatic regenerative processes. The triggering receptor expressed myeloid cells 2 (TREM-2) predominantly in non-parenchymal inhibits Toll-like signalling, protecting the from various hepatotoxic injuries, yet its role poorly defined. Here, we investigated impact TREM-2 regeneration hepatocarcinogenesis. Design expression was analysed...
Abstract Sialic acid-binding Ig-like lectin 15 (Siglec-15) is an immune modulator and emerging cancer immunotherapy target. However, limited understanding of its structure mechanism action restrains the development drug candidates that unleash full therapeutic potential. In this study, we elucidate crystal Siglec-15 binding epitope via co-crystallization with anti-Siglec-15 blocking antibody. Using saturation transfer-difference nuclear magnetic resonance (STD-NMR) spectroscopy molecular...
Defining the trajectory of cells during differentiation and disease is key for uncovering mechanisms driving cell fate identity. However, trajectories human remain largely unexplored due to challenges studying them with samples. In this study, we investigate proteome iPSCs hepatic stellate (diHSCs) identify RORA as a transcription factor governing metabolic reprogramming HSCs necessary diHSCs' commitment, identity, activation. Using deficient pharmacologic interventions, show that required...
Chemical burns to the eye cause extensive ocular surface damage, often resulting in permanent visual impairment. The amniotic membrane (AM), with its regenerative properties, has shown great potential treating such injuries. Recent innovations, including hydrogel-based delivery systems, have been developed improve drug retention and promote corneal healing. This study aimed evaluate whether hydrogels loaded proteins from human AM could enhance wound healing effectively. Alkaline (8 mm...
Global proteome analysis of protein glycosylation is a major challenge due to the inherent heterogeneous and diverse nature this post-translational modification. It therefore common enzymatically remove glycans attached or peptide chains prior mass spectrometric analysis, thereby reducing complexity facilitating site determinations. Here, we have used two different enzymatic deglycosylation strategies for N-glycosylation analysis. (1) Removal entire N-glycan by peptide-N-glycosidase (PNGase)...
Plasma membrane proteins are displayed through diverse mechanisms, including anchoring in the extracellular leaflet via glycosylphosphatidylinositol (GPI) molecules. GPI-anchored (GPI-APs) a functionally and structurally protein family, their importance is well-recognized as they candidate cell surface biomarker molecules with potential diagnostic therapeutic applications molecular medicine. GPI-APs have also attracted interest plant biotechnology because of role root development remodeling....
The endometrial fluid is a noninvasive sample which contains numerous secreted proteins representative of function and reflects the state endometrium. In this study, we describe, for first time, comprehensive catalogue during secretory phase menstrual cycle. To achieve objective, three different but complementary strategies were used: First, in-solution digestion followed by reverse high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS); second, protein...
Plasma rich in growth factors (PRGF®-Endoret®) is an autologous technology that contains a set of proteins specifically addressed to wound healing and tissue regeneration. The scaffold formed by using this clot mainly composed fibrin protein, forming three-dimensional (3D) macroscopic network. This biomaterial easily obtained biotechnological means from blood can be used range situations help Although the main constituent scaffold, little known about other interacting may act as adjuvants...
Primary liver cancer (HCC) is recognized as the fifth most common neoplasm and second leading cause of death worldwide. Most risk factors are known, molecular pathogenesis has been widely studied in past decade; however, underlying mechanisms remain to be unveiled, they will facilitate definition novel biomarkers clinical targets for more effective patient management. We utilize B/D-HPP popular protein strategy. report a list proteins that have highly cocited with expression "liver cancer"....
CD5-like (CD5L) is a soluble scavenger cysteine-rich protein that modulates inflammatory responses. We studied the involvement of CD5L in liver cancer. Immunohistochemistry (IHC) 60 hepatocellular carcinomas and 34 adjacent nontumor livers, showed staining was higher tumor than tissue (Mann-Whitney test; P = 0.0039). High correlated with elevated proliferation (Ki67, linear regression; < 0.0001) lower patient event-free survival (log-rank; 0.0185). Accordingly, expression detected cancer...