A5017625141- Acute Lymphoblastic Leukemia research
- Chronic Myeloid Leukemia Treatments
- RNA modifications and cancer
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Bioinformatics and Genomic Networks
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA Interference and Gene Delivery
- Microbial Metabolic Engineering and Bioproduction
- Childhood Cancer Survivors' Quality of Life
- Gene expression and cancer classification
- Computational Drug Discovery Methods
- DNA Repair Mechanisms
- Lymphoma Diagnosis and Treatment
- RNA and protein synthesis mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Advanced Proteomics Techniques and Applications
- T-cell and Retrovirus Studies
- T-cell and B-cell Immunology
- Biomedical Text Mining and Ontologies
- Cancer, Lipids, and Metabolism
Centro de Investigación del Cáncer
2012-2024
Universidad de Salamanca
2015-2024
Consejo Superior de Investigaciones Científicas
2015-2024
Children's Hospital of Philadelphia
2024
Bioinformatics Institute
2017-2020
Instituto de Investigación Biomédica de Salamanca
2016-2020
Centro de Investigación Biomédica en Red de Cáncer
2018
Abstract RNA-seq is currently considered the most powerful, robust and adaptable technique for measuring gene expression transcription activation at genome-wide level. As analysis of data complex, it has prompted a large amount research on algorithms methods. This resulted in substantial increase number options available each step analysis. Consequently, there no clear consensus about appropriate pipelines that should be used to analyse data. In present study, 192 using alternative methods...
The collection and integration of all the known protein–protein physical interactions within a proteome framework are critical to allow proper exploration protein interaction networks that drive biological processes in cells at molecular level. APID Interactomes is public resource data (http://apid.dep.usal.es) provides comprehensive curated `protein interactomes' for more than 1100 organisms, including 30 species with 500 interactions, derived from experimentally detected protein-to-protein...
Abstract Newly growing evidence highlights the essential role that epitranscriptomic marks play in development of many cancers; however, little is known about and implications altered epitranscriptome deposition prostate cancer. Here, we show transfer RNA N 7 -methylguanosine (m G) transferase METTL1 highly expressed primary advanced tumours. Mechanistically, find depletion causes loss m G tRNA methylation promotes biogenesis a novel class small non-coding RNAs derived from 5'tRNA fragments....
Abstract Earlier in the past century, infections were regarded as most likely cause of childhood B-cell precursor acute lymphoblastic leukemia (pB-ALL). However, there is a lack relevant biologic evidence supporting this hypothesis. We present vivo genetic mechanistically connecting inherited susceptibility to pB-ALL and postnatal by showing that was initiated Pax5 heterozygous mice only when they exposed common pathogens. Strikingly, these murine pB-ALLs closely resemble human disease....
APID (Agile Protein Interactomes DataServer) is an interactive web server that provides unified generation and delivery of protein interactomes mapped to their respective proteomes. This resource a new, fully redesigned includes comprehensive collection for more than 400 organisms (25 which include 500 interactions) produced by the integration only experimentally validated protein-protein physical interactions. For each interaction (PPI) currently reported information about its experimental...
// José Luis Ordóñez 1,4,* , Ana Teresa Amaral Angel M. Carcaboso 2 David Herrero-Martín 3 María del Carmen García-Macías 4 Vicky Sevillano Diego Alonso Guillem Pascual-Pasto Laura San-Segundo Monica Vila-Ubach Telmo Rodrigues Susana Fraile Cristina Teodosio Agustín Mayo-Iscar 5 Miguel Aracil 6 Carlos Galmarini Oscar Tirado Jaume Mora and Enrique de Álava 1,4 1 Laboratory of Molecular Pathology, Instituto Biomedicina Sevilla (IBiS), Hospital Universitario Virgen Rocio/CSIC/Universidad...
Abstract ETV6-RUNX1 is associated with the most common subtype of childhood leukemia. As few carriers develop precursor B-cell acute lymphocytic leukemia (pB-ALL), underlying genetic basis for development full-blown remains to be identified, but appearance cases in time-space clusters keeps infection as a potential causal factor. Here, we present vivo evidence mechanistically connecting preleukemic expression hematopoetic stem cells/precursor cells (HSC/PC) and postnatal infections...
Systems biologists study interaction data to understand the behaviour of whole cell systems, and their environment, at a molecular level. In order effectively achieve this goal, it is critical that researchers have high quality datasets available them, in standard format, also suite tools with which analyse such form experimentally testable hypotheses from them. The PSI-MI XML interchange format was initially published 2004, expanded 2007 enable download data. PSI-XML2.5 designed describe...
Preleukemic clones carrying
Article7 June 2018Open Access Transparent process Lmo2 expression defines tumor cell identity during T-cell leukemogenesis Idoia García-Ramírez Experimental Therapeutics and Translational Oncology Program, Instituto de Biología Molecular y Celular del Cáncer, CSIC-USAL, Salamanca, Spain Institute of Biomedical Research Salamanca (IBSAL), Search for more papers by this author Sanil Bhatia Department Pediatric Oncology, Hematology Clinical Immunology, Medical Faculty, Heinrich-Heine University...
Cutaneous squamous cell carcinoma (CSCC) is the second most widespread cancer in humans and its incidence rising. These tumours can evolve as diseases of poor prognosis, therefore it important to identify new markers better predict clinical evolution.We aimed expression pattern microRNAs (miRNAs or miRs) at different stages skin progression a panel murine lines. Owing increasing importance miRNAs pathogenesis cancer, we considered possibility that could help define prognosis CSCC evaluate...
Cancer-associated fibroblasts (CAFs) are key regulators of the tumor microenvironment, promoting progression through extracellular matrix (ECM) remodeling and paracrine signaling, but signaling pathways controlling CAF function remain incompletely defined. Here, we demonstrate that RAS-PI3K plays a central role in activation ECM by collagen crosslinking, fibronectin organization, glycoprotein deposition at least partially YAP. Disruption interaction CAFs leads to structurally mechanically...
Abstract Background An essential question in cancer is why individuals with the same disease have different clinical outcomes. Progress toward a more personalized medicine patients requires taking into account underlying heterogeneity at molecular levels. Results Here, we present model which there are complex interactions cellular and systemic levels that for of susceptibility to evolution ERBB2-positive breast cancers. Our based on our analyses cohort mice characterized by heterogeneous...
Metabolic reprogramming of germinal center B cells requires the activity R-Ras2.
Abstract The prerequisite to prevent childhood B-cell acute lymphoblastic leukemia (B-ALL) is decipher its etiology. current model suggests that infection triggers B-ALL development through induction of activation-induced cytidine deaminase (AID; also known as AICDA) in precursor B-cells. This evidence has been largely acquired the use ex vivo functional studies. However, whether this mechanism governs native non-transplant unknown. Here we show that, surprisingly, AID genetic deletion does...
T cells form immunological synapses with professional antigen-presenting (APCs) resulting in cell activation and the acquisition of peptide antigen-MHC (pMHC) complexes from plasma membrane APC. They thus become APCs themselves. We investigate functional outcome T-T antigen presentation by CD4 find that (Tpres) predominantly differentiate into regulatory (Treg), whereas have been stimulated Tpres Th17 pro-inflammatory cells. Using mice deficient pMHC uptake cells, we show is important for...
Preventing development of childhood B-cell acute lymphoblastic leukemia (B-ALL), a disease with devastating effects, is longstanding and unsolved challenge. Heterozygous germline alterations in the PAX5 gene can lead to B-ALL upon accumulation secondary mutations affecting JAK/STAT signaling pathway. Preclinical studies have shown that this malignant transformation occurs only under immune stress such as exposure infectious pathogens. Here we show Pax5+/- mice transient, early-life...
Abstract PAX5 is one of the most frequently mutated genes in B-cell acute lymphoblastic leukemia (B-ALL), and children with inherited preleukemic mutations are at a higher risk developing disease. Abnormal profiles inflammatory markers have been detected neonatal blood spot samples who later developed B-ALL. However, how signals contribute to B-ALL development unclear. Here, we demonstrate that Pax5 heterozygosis, presence infections, results enhanced production cytokine interleukin-6...
Abstract SNAI2 overexpression appears to be associated with poor prognosis in breast cancer, yet it remains unclear which cancer subtypes this occurs. Here we show that excess is a of luminal B HER2+/ERBB2+ cancers expression the stroma but not epithelium correlates tumor proliferation. To determine how stromal might influence HER2+ behavior, Snai2-deficient mice were crossed mouse line carrying ErbB2/Neu protooncogene generate cancer. Tumors generated model expressed epithelium, allowing...
Abstract The initial steps of B-cell acute lymphoblastic leukemia (B-ALL) development usually pass unnoticed in children. Several preclinical studies have shown that exposure to immune stressors triggers the transformation preleukemic B cells full-blown B-ALL, but how this takes place is still a longstanding and unsolved challenge. Here we show dysregulation innate immunity plays driving role clonal evolution pre-malignant Pax5 +/− precursors toward leukemia. Transcriptional profiling...
Cancer is a complex disease affecting millions of people worldwide, with over hundred clinically approved drugs available. In order to improve therapy, treatment, and response, it essential draw better maps the targets cancer possible side interactors. This study presents large-scale screening method find associations human genes. The analysis focused on current collection Food Drug Administration (FDA)-approved (which includes about one chemicals). approach integrates global gene-expression...
In the last years, use of thrombopoietin receptor agonists (TPO-RA), eltrombopag and romiplostim, has improved management immune thrombocytopenia (ITP). Moreover, is also active in patients with aplastic anemia myelodysplastic syndrome. However, their mechanisms action signaling pathways still remain controversial. order to gain insight into underlying therapy, a gene expression profile (GEP) analysis treated this drug was carried out. Fourteen chronic ITP were studied by means microarrays...