A5051350486- Liver Disease Diagnosis and Treatment
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Liver Diseases and Immunity
- Pediatric Hepatobiliary Diseases and Treatments
- Genetic and Kidney Cyst Diseases
- Endoplasmic Reticulum Stress and Disease
- Diet, Metabolism, and Disease
- Liver Disease and Transplantation
- RNA modifications and cancer
- Gallbladder and Bile Duct Disorders
- Hepatitis C virus research
- MicroRNA in disease regulation
- Metabolomics and Mass Spectrometry Studies
- Alcohol Consumption and Health Effects
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- SARS-CoV-2 detection and testing
- Lipid metabolism and disorders
- Pancreatic and Hepatic Oncology Research
- Peptidase Inhibition and Analysis
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer, Lipids, and Metabolism
- Liver physiology and pathology
- Cancer-related gene regulation
- Biosensors and Analytical Detection
Biogipuzkoa Health Research Institute
2015-2023
Marqués de Valdecilla University Hospital
2020-2023
Instituto de Investigación Marqués de Valdecilla
2020-2023
University of the Basque Country
2015-2023
Ghent University Hospital
2022
Universidad de Cantabria
2021
Ikerbasque
2018-2020
NIHR Clinical Research Network
2019
Lymphatic Education & Research Network
2019
Universidad de Navarra
2018
Cholangiocarcinoma (CCA) includes a heterogeneous group of biliary cancers with poor prognosis. Several conditions, such as primary sclerosing cholangitis (PSC), are risk factors. Noninvasive differential diagnosis between intrahepatic CCA and hepatocellular carcinoma (HCC) is sometimes difficult. Accurate noninvasive biomarkers for PSC, CCA, HCC not available. In the search novel biomarkers, serum extracellular vesicles (EV) were isolated from (n = 43), PSC 30), or 29) patients healthy...
Cholangiocarcinoma (CCA) is a rare and heterogeneous biliary cancer, whose incidence related mortality increasing. This study investigates the clinical course of CCA subtypes (intrahepatic [iCCA], perihilar [pCCA], distal [dCCA]) in pan-European cohort.The ENSCCA Registry multicenter observational study. Patients were included if they had histologically proven diagnosis between 2010-2019. Demographic, histomorphological, biochemical, studies performed.Overall, 2,234 patients enrolled...
Early and differential diagnosis of intrahepatic cholangiocarcinoma (iCCA) hepatocellular carcinoma (HCC) by noninvasive methods represents a current clinical challenge. The analysis low‐molecular‐weight metabolites new high‐throughput techniques is strategy for identifying biomarkers. Here, we have investigated whether serum metabolome can provide useful biomarkers in the iCCA HCC could discriminate from HCC. Because primary sclerosing cholangitis (PSC) risk factor CCA, metabolic profiles...
Objective Liver injury impacts hepatic inflammation in part via Toll-like receptor (TLR) signalling. Triggering expressed on myeloid cells 2 (TREM-2) modulates TLR4-mediated bone marrow (BM)-derived macrophages but its function liver is unknown. Here we hypothesised that the anti-inflammatory effects of TREM-2 TLR signalling may limit injury. Design expression was analysed livers humans with various forms compared control individuals. Acute and chronic models were performed wild type Trem-2...
Abstract Epigenetic modifications such as DNA and histone methylation functionally cooperate in fostering tumor growth, including that of hepatocellular carcinoma (HCC). Pharmacological targeting these mechanisms may open new therapeutic avenues. We aimed to determine the efficacy potential mechanism action our dual G9a histone‐methyltransferase DNA‐methyltransferase 1 (DNMT1) inhibitor human HCC cells their crosstalk with fibrogenic cells. The expression DNMT1 , along molecular adaptor...
Objective Receptor-interacting protein kinase 3 (RIPK3) is a key player in necroptosis execution and an emerging metabolic regulator, whose contribution to non-alcoholic fatty liver disease (NAFLD) controversial. We aimed clarify the impact of RIPK3 signalling pathogenesis human experimental NAFLD. Design levels were evaluated two large independent cohorts patients with biopsy proven NAFLD diagnosis correlated clinical biochemical parameters. Wild-type (WT) or Ripk3 -deficient ( −/− ) mice...
: Cholangiocarcinoma (CCA) comprises a group of heterogeneous biliary cancers with dismal prognosis. The etiologies most CCAs are unknown, but primary sclerosing cholangitis (PSC) is risk factor. Non-invasive diagnosis CCA challenging and accurate biomarkers lacking. We aimed to characterize the transcriptomic profile serum urine extracellular vesicles (EVs) from patients CCA, PSC, ulcerative colitis (UC), healthy individuals. Serum EVs were isolated by serial ultracentrifugations...
Non-alcoholic fatty liver disease (NAFLD) is affecting more people globally. Indeed, NAFLD a spectrum of metabolic dysfunctions that can progress to hepatocellular carcinoma (NAFLD-HCC). This development occur in non-cirrhotic and thus, often lack clinical surveillance. The aim this study was develop non-invasive surveillance method for NAFLD-HCC.
Primary biliary cholangitis (PBC) is a chronic cholestatic liver disease associated with autoimmune phenomena targeting intrahepatic bile duct cells (cholangiocytes). Although its etiopathogenesis remains obscure, development of antimitochondrial autoantibodies against pyruvate dehydrogenase complex E2 common feature. MicroRNA (miR) dysregulation occurs in and immune PBC patients, but functional relevance largely unknown. We previously reported that miR‐506 overexpressed cholangiocytes...
Background and Aims Intrahepatic cholangiocarcinoma (iCCA) with liver metastases is perceived to have a poor prognosis, but the American Joint Committee on Cancer (AJCC) classifies them as early stage in absence of lymph nodes or extrahepatic spread. Approach Results Patients iCCA from European Network for Study Cholangiocarcinoma (ENS‐CCA) Surveillance, Epidemiology, End (SEER) registries survival/staging (AJCC v.7) data were eligible. Modified staging was used (mAJCC v.7): group A: stages...
Background: Nonalcoholic fatty liver disease (NAFLD) is the most common chronic where biopsy remains gold standard for diagnosis. Here we aimed to evaluate role of circulating adiponectin, leptin, and insulin-like growth factor 1 (IGF-1) levels as non-invasive NAFLD biomarkers assess their correlation with metabolome. Materials Methods: Leptin, IGF-1 serum were measured by ELISA in two independent cohorts biopsy-proven obese patients healthy-liver controls (discovery: 38 NAFLD, 13 controls;...
Abstract Background and Aims The mechanisms governing the progression of non‐alcoholic fatty liver disease (NAFLD) towards steatohepatitis (NASH) hepatocellular carcinoma (HCC) remain elusive. Here, we evaluated role hsa‐miRNA‐21‐5p in NASH‐related hepatocarcinogenesis. Methods Hepatic hsa‐miR‐21‐5p expression was two cohorts patients with biopsy‐proven NAFLD ( n = 199) or HCC 366 11 NAFLD‐HCC). Serum/liver metabolomic profiles were correlated obese patients. Wild‐type (WT) Mir21 KO mice fed...
Cholangiocarcinoma (CCA) is a devastating disease often detected at advanced stages when surgery cannot be performed. Conventional and targeted systemic therapies perform poorly, therefore effective drugs are urgently needed. Different epigenetic modifications occur in CCA contribute to malignancy. Targeting mechanisms may thus open therapeutic opportunities. However, such as DNA histone methylation coexist cooperate carcinogenesis. We tested the efficacy mechanism of action class dual G9a...
Abstract Background & Aims Polycystic liver diseases (PLDs) are genetic disorders characterized by progressive development of multiple biliary cysts. Recently, novel PLD‐causative genes, encoding for endoplasmic reticulum (ER)‐resident proteins involved in protein biogenesis and transport, were identified. We hypothesized that aberrant proteostasis contributes to PLD pathogenesis, representing a potential therapeutic target. Methods ER stress was analysed at transcriptional (qPCR),...
Abstract The simplicity and low cost of rapid point-of-care tests greatly facilitate large-scale population testing, which can contribute to controlling the spread COVID-19 virus. We evaluated applicability a self-testing strategy for SARS-CoV2 in population-based, cross-sectional study Cantabria, Spain, between April May 2020. For strategy, participants received necessary material self-collection blood performance antibody test using lateral flow immunoassay at home without supervision...