A5033255398- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Lipid metabolism and biosynthesis
- Chromatography in Natural Products
- Natural product bioactivities and synthesis
- Lipid Membrane Structure and Behavior
- Traditional Chinese Medicine Analysis
- Advanced Proteomics Techniques and Applications
- Plant biochemistry and biosynthesis
- Computational Drug Discovery Methods
- Advanced Chemical Sensor Technologies
- Microbial Metabolic Engineering and Bioproduction
- Traditional Chinese Medicine Studies
- Cancer, Lipids, and Metabolism
- Lysosomal Storage Disorders Research
- Sphingolipid Metabolism and Signaling
- Cannabis and Cannabinoid Research
- Pharmacogenetics and Drug Metabolism
- Photosynthetic Processes and Mechanisms
- Phytochemistry and Biological Activities
- Microbial Natural Products and Biosynthesis
- Spectroscopy and Chemometric Analyses
- Analytical Methods in Pharmaceuticals
- Pancreatic function and diabetes
University of Massachusetts Chan Medical School
2023-2024
Waters (United States)
2014-2023
Kansas State University
2006-2012
Pacific Northwest National Laboratory
2010-2012
Washington State University
2012
Physical Sciences (United States)
2012
University of Strathclyde
2007
University of Chicago
2007
Uppsala University
2002-2006
Stockholm University
2005
Dengue virus causes ∼50–100 million infections per year and thus is considered one of the most aggressive arthropod-borne human pathogen worldwide. During its replication, dengue induces dramatic alterations in intracellular membranes infected cells. This phenomenon observed both vector-derived Using high-resolution mass spectrometry mosquito cells, we show that this membrane remodeling directly linked to a unique lipid repertoire induced by infection. Specifically, 15% metabolites detected...
Metabolic profiling studies aim to achieve broad metabolome coverage in specific biological samples. However, wide has proven difficult achieve, mostly because of the diverse physicochemical properties small molecules, obligating analysts seek multiplatform and multimethod approaches. Challenges are even greater when it comes applications tissue samples, where lysis metabolite extraction can induce significant systematic variation composition. We have developed a pipeline for obtaining...
An ultraperformance LC (UPLC) method for the separation of different lipid molecular species and isomers using a stationary phase incorporating charged surface hybrid (CSH) technology is described. The resulting enhanced possibilities are demonstrated standards human plasma extracts. Lipids were extracted from samples with Bligh Dyer method. Separation lipids was achieved on 100 × 2.1 mm inner diameter CSH C18 column gradient elution aqueous-acetonitrile-isopropanol mobile phases containing...
Abstract Molecular networking has become a key method used to visualize and annotate the chemical space in non-targeted mass spectrometry-based experiments. However, distinguishing isomeric compounds quantitative interpretation are currently limited. Therefore, we created Feature-based Networking (FBMN) as new analysis Global Natural Products Social (GNPS) infrastructure. FBMN leverages feature detection alignment tools enhance analyses isomer distinction, including from ion-mobility...
Intracellular phospholipase A2 (PLA2) plays an important role in regulating oxylipin biosynthesis mammals, but the molecular and biochemical nature of intracellular PLA2 is not well understood plants. Arabidopsis thaliana gene At1g61850 (AtPLAI) encodes a 140-kDa protein that most similar to mammalian calcium-independent PLA2, additionally contains leucine-rich repeats Armadillo repeats. AtPLAI hydrolyzes phospholipids at both sn-1 sn-2 positions, prefers galactolipids as substrates....
Abstract Tocopherols (vitamin E) are synthesized in plastids and have long been assumed to essential functions restricted these organelles. We previously reported that the vitamin e-deficient2 (vte2) mutant of Arabidopsis thaliana is defective transfer cell wall development photoassimilate transport at low temperature (LT). Here, we demonstrate LT-treated vte2 has a distinct composition polyunsaturated fatty acids (PUFAs): lower levels linolenic acid (18:3) higher linoleic (18:2) compared...
Correlations between the dimensions of a 2-D separation create trend lines that depend on structural or chemical characteristics compound class and thus facilitate classification unknowns. This broadly applies to conventional ion mobility spectrometry (IMS)/mass (MS), where major biomolecular classes (e.g., lipids, peptides, nucleotides) occupy different line domains. However, strong correlation IMS MS separations for ions same charge has impeded finer distinctions. Differential (or FAIMS)...
Harlequin ichthyosis is a congenital scaling syndrome of the skin in which affected infants have epidermal hyperkeratosis and defective permeability barrier. Mutations gene encoding member ABCA transporter family, ABCA12, been linked to harlequin ichthyosis, but molecular function protein unknown. To investigate activity we generated Abca12 null mice analyzed impact on lipid content. Abca12-/- are born with thickened epidermis die shortly after birth, as water rapidly evaporates from their...
Current optimum medical treatments have had limited success in the primary prevention of cardiovascular events, underscoring need for new pharmaceutical targets and enhanced understanding mechanistic metabolic dysregulation. Here, we use a combination novel profiling methodologies, based on ultra-performance liquid chromatography coupled to mass spectrometry (UPLC–MS) followed by chemometric modeling, data integration, pathway mapping, create systems-level atlas atherogenesis. We apply this...
Abstract Oat kernels were extracted with methanol, and glycolipid‐enriched fractions prepared using silica solid phase extraction. Using direct infusion electrospray ionization (ESI) tandem mass spectrometry (MS), high performance liquid chromatography (HPLC)‐ESI‐MS, HPLC‐atmospheric pressure chemical (APCI)‐MS, we confirmed previous reports that digalactosyldiacylglycerol (DGDG) was the most abundant glycolipid in oat a report of presence DGDG mono‐estolide kernels. In current study also...
Abstract Motivation: Liquid chromatography–mass spectrometry-based metabolomics has gained importance in the life sciences, yet it is not supported by software tools for high throughput identification of metabolites based on their fragmentation spectra. An algorithm (ISIS: silico software) and its implementation are presented show great promise generating spectra lipids purpose structural identification. Instead using chemical reaction rate equations or rules-based libraries, uses machine...
The need for rapid and efficient high throughput metabolic phenotyping (metabotyping) in metabolomic/metabonomic studies often requires compromises to be made between analytical speed metabolome coverage. Here the effect of column length (150, 75 30 mm) gradient duration (15, 7.5 3 min respectively) on number features detected when untargeted profiling human urine using reversed-phase ultra performance chromatography with, without, ion mobility spectrometry, has been examined. As would...
Analysis of the polar lipids Toxoplasma gondii by electrospray ionization tandem mass spectrometry provides a detailed picture lipid molecular species this parasitic protozoan. Most notably, T. contains relatively high level, estimated to about 2% total lipid, ceramide phosphoethanolamine. The phosphoethanolamine has fatty amide profile with only 16- and 18-carbon species. Compared host fibroblasts in which it was grown, also higher levels phosphatidylcholine but lower sphingomyelin...
Acer truncatum is an important ornamental, edible, and medicinal plant resource in China. Previous phytochemical research has focused on the leaf (AL) due to its long history as a tea for health. Other parts such branch (ABr), bark (ABa), fruit (AF), root (AR) have drawn little attention regarding their metabolites bioactivities. The strategy of in-house chemical library combined with Progenesis QI informatics platform was applied characterize metabolites. A total 98 compounds were...
Eleutherococcus senticosus Maxim. belongs to the Araliaceae family. Phytochemical studies reveal that E. leaves contain triterpene glycosides along with organic acid derivatives and flavonoid compounds. It is believed similar ginseng because they come from same family both saponins. have been developed as a functional beverage called ci-wu-jia tea in recent years. Triterpene are difficult identify by ultraviolet (UV) detection contents of these compounds low leaves. In this study, sensitive...
Metabolomics is a powerful phenotyping platform with potential for high-throughput analyses. The primary technology metabolite profiling mass spectrometry. In recent years, the coupling of spectrometry ion mobility (IMS) has offered promise faster analysis time and greater resolving power. Our understanding impact IMS on field metabolomics limited by availability comprehensive experimental data. this analysis, we use probabilistic approach to enumerate strengths limitations, present future,...
Establishment of sensitive methods for the detection cellular sterols and their derivatives is a critical step in developing comprehensive lipidomics technology. We demonstrate that electrospray ionization tandem (triple quadrupole) mass spectrometry (ESI-MS/MS) an efficient method monitoring steryl glucosides (SG) acyl (ASG). Comparison analysis SG ASG by ESI-MS/MS with gas chromatography flame (GC-FID) shows two yield similar molar compositions. These data response per amount sterol...