Ricardo Silva
A5063348766- Metabolomics and Mass Spectrometry Studies
- Gut microbiota and health
- Analytical Chemistry and Chromatography
- Contact Dermatitis and Allergies
- Dermatology and Skin Diseases
- Cystic Fibrosis Research Advances
- Genomics and Phylogenetic Studies
- Skin Protection and Aging
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Plant and animal studies
- Computational Drug Discovery Methods
- Advanced Chemical Sensor Technologies
- Isotope Analysis in Ecology
- Bioinformatics and Genomic Networks
- Plant biochemistry and biosynthesis
- Mass Spectrometry Techniques and Applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Spectroscopy and Chemometric Analyses
- Insect and Pesticide Research
- Microbial Community Ecology and Physiology
- Surface Roughness and Optical Measurements
- Bioactive Natural Diterpenoids Research
- Water Quality Monitoring and Analysis
Universidade de São Paulo
2015-2025
University of Coimbra
2025
Cell Medica (Switzerland)
2024
Institute for Biotechnology and Bioengineering
2024
Universidade de Ribeirão Preto
2018-2023
Natura (Brazil)
2023
University of California, San Diego
2016-2022
Universidade Federal de Minas Gerais
2022
Instituto Superior Técnico
2022
University of Montana
2015-2021
It is a common problem in natural product therapeutic lead discovery programs that despite good bioassay results the initial extract, active compound(s) may not be isolated during subsequent bioassay-guided purification. Herein, we present concept of bioactive molecular networking to find candidate molecules directly from fractionated extracts. By employing tandem mass spectrometry, it possible accelerate dereplication using prior isolation compounds, and also expose potentially bioactivity...
The annotation of small molecules is one the most challenging and important steps in untargeted mass spectrometry analysis, as our biological interpretations rely on structural annotations. Molecular networking has emerged a structured way to organize mine data from tandem (MS/MS) experiments been widely applied propagate However, propagation done through manual inspection MS/MS spectra connected spectral networks only possible when reference library spectrum available. One alternative...
Use of skin personal care products on a regular basis is nearly ubiquitous, but their effects molecular and microbial diversity the are unknown. We evaluated impact four beauty (a facial lotion, moisturizer, foot powder, deodorant) 11 volunteers over 9 weeks.Mass spectrometry 16S rRNA inventories revealed decreases in chemical as well bacterial archaeal halting deodorant use. Specific compounds from used before study remain detectable with half-lives 0.5-1.9 weeks. The powder increased...
From offense to defense Venom in snakes is largely used subdue and/or kill prey, and most venoms have clear actions that facilitate death or paralysis. In one group of snakes, however, venom has evolved shifted from predation protection. Specifically, three different lineages “spitting” deter predators. Kazandjian et al. show similar adaptions occurred within these transform cytotoxic components into a mixture acts on mammalian sensory neurons causes pain. The authors argue increased led...
In order to expedite the rapid and efficient discovery isolation of novel specialized metabolites, while minimizing waste resources on rediscovery known compounds, it is crucial develop approaches for strain prioritization, dereplication, assessment favored cultivation extraction conditions. Herein we interrogated bacterial strains by systematically evaluating parameters with LC-MS/MS analysis subsequent dereplication through Global Natural Product Social Molecular Networking (GNPS)...
Dissolved organic matter (DOM) is arguably one of the most complex exometabolomes on earth, and comprised thousands compounds, that together contribute more than 600×1015 g carbon. This reservoir primarily product interactions between upper ocean's microbial food web, yet abiotic processes occur over millennia have also modified many its molecules. The compounds within this play important roles in determining rate extent element exchange inorganic reservoirs marine biosphere, while mediating...
Abstract Molecular networking has become a key method used to visualize and annotate the chemical space in non-targeted mass spectrometry-based experiments. However, distinguishing isomeric compounds quantitative interpretation are currently limited. Therefore, we created Feature-based Networking (FBMN) as new analysis Global Natural Products Social (GNPS) infrastructure. FBMN leverages feature detection alignment tools enhance analyses isomer distinction, including from ion-mobility...
Increasing appreciation of the gut microbiome's role in health motivates understanding molecular composition human feces. To analyze such complex samples, we developed a platform coupling targeted and untargeted metabolomics. The approach is facilitated through split flow from one UPLC, joint timing triggered by contact closure relays, script to retrieve data. It designed detect specific metabolites interest with high sensitivity, allows for correction information, enables better...
Imagine a scenario where personal belongings such as pens, keys, phones, or handbags are found at an investigative site. It is often valuable to the team that trying trace back individual understand their habits, even when DNA evidence also available. Here, we develop approach translate chemistries recovered from objects phones into lifestyle sketch of owner, using mass spectrometry and informatics approaches. Our results show phones' reflect personalized profile. The collective repertoire...
Plants produce a myriad of specialized metabolites to overcome their sessile habit and combat biotic as well abiotic stresses. Evolution has shaped the diversity metabolites, which then drives many other aspects plant biodiversity. However, until recently, large-scale studies investigating in an evolutionary context have been limited by impossibility identifying chemical structures hundreds thousands compounds time-feasible manner. Here we introduce workflow for large-scale, semi-automated...
Multi-omics methods have greatly advanced our understanding of the biological organism and its microbial associates. However, they are not routinely used in clinical or industrial applications, due to length time required generate analyze omics data. Here, we applied a novel integrated pipeline for analysis human environmental samples under 48 h. Human subjects that ferment their own foods provided swab from skin, feces, oral cavity, fermented foods, household surfaces assess impact home...
Studies of the cystic fibrosis (CF) lung microbiome have consistently shown that function decline is associated with decreased microbial diversity due to dominance opportunistic pathogens. However, how this phenomenon reflected in metabolites and chemical environment secretions remains poorly understood. Here we investigated molecular composition CF sputum samples using 16S rRNA gene amplicon sequencing untargeted tandem mass spectrometry determine their interrelationships associations...
In most species of social insect the queen signals her presence to workers via pheromones. Worker responses pheromones include retinue formation around queen, inhibition cell production and suppression worker ovary activation. Here we show that signal Brazilian stingless bee Friesella schrottkyi is a mixture cuticular hydrocarbons. Stingless bees are therefore similar ants, wasps bumble bees, but differ from honey in which queen's mostly comprises volatile compounds originating mandibular...