A5037467457- Developmental Biology and Gene Regulation
- Venomous Animal Envenomation and Studies
- Zebrafish Biomedical Research Applications
- Insect and Pesticide Research
- Congenital heart defects research
- Amphibian and Reptile Biology
- Biochemical and Structural Characterization
- Morphological variations and asymmetry
- Paleontology and Evolutionary Biology
- Fish Ecology and Management Studies
- Evolution and Paleontology Studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Coronary Artery Anomalies
- Animal Genetics and Reproduction
- Animal Behavior and Reproduction
- Healthcare and Venom Research
- Pluripotent Stem Cells Research
- Neuroendocrine regulation and behavior
- melanin and skin pigmentation
- Bat Biology and Ecology Studies
- Chromosomal and Genetic Variations
- Nanoparticles: synthesis and applications
- Congenital Heart Disease Studies
- Genetic diversity and population structure
- Birth, Development, and Health
Leiden University
2016-2025
Los Alamos National Laboratory
2023
Naturalis Biodiversity Center
2022
The University of Queensland
2020
University of Washington
1988-2019
Seattle University
2019
Leiden University Medical Center
2007
United States Naval Research Laboratory
2004
St George's, University of London
1995-2003
St George's Hospital
1996-2003
ABSTRACT We show here that bone morphogenetic protein 2 (BMP-2) is involved in patterning the developing chick limb. During early stages of limb development, mesenchymal expression Bmp-2 gene restricted to posterior part bud, a domain colocalizes with polarizing region. The region group cells at margin bud can respecify anteroposterior axis when grafted anteriorly and activate genes HoxD complex. dissect possible roles BMP-2 signalling pathway by manipulating wing bud. Retinoic acid...
Significance Snake venoms are toxic protein cocktails used for prey capture. To investigate the evolution of these complex biological weapon systems, we sequenced genome a venomous snake, king cobra, and assessed composition venom gland expressed genes, small RNAs, secreted proteins. We show that regulatory components secretory system may have evolved from pancreatic origin toxin genes were co-opted by distinct genomic mechanisms. After co-option, important capture massively expanded gene...
Snakes possess many extreme morphological and physiological adaptations. Identification of the molecular basis these traits can provide novel understanding for vertebrate biology medicine. Here, we study snake using genome sequence Burmese python (Python molurus bivittatus), a model metabolic adaptation. We compare king cobra genomes along with genomic samples from other snakes perform transcriptome analysis to gain insights into phenotypes python. discovered rapid massive transcriptional...
From offense to defense Venom in snakes is largely used subdue and/or kill prey, and most venoms have clear actions that facilitate death or paralysis. In one group of snakes, however, venom has evolved shifted from predation protection. Specifically, three different lineages “spitting” deter predators. Kazandjian et al. show similar adaptions occurred within these transform cytotoxic components into a mixture acts on mammalian sensory neurons causes pain. The authors argue increased led...
Low oxygen levels (hypoxia) play a role in clinical conditions such as stroke, chronic ischemia, and cancer. To better understand these diseases, it is crucial to study the responses of vertebrates hypoxia. Among vertebrates, some teleosts have developed ability adapt extremely low levels. We studied long-term adaptive hypoxia adult zebrafish. used zebrafish that survived severe hypoxic for 3 wk showed behavioral phenotypic changes. cDNA microarrays investigate hypoxia-induced changes...
Background In the drug discovery pipeline, safety pharmacology is a major issue. The zebrafish has been proposed as model that can bridge gap in this field between cell assays (which are cost-effective, but low data content) and rodent high content, less cost-efficient). However, only likely to be useful if they shown have predictive power. We examined issue by assaying 60 water-soluble compounds representing range of chemical classes toxicological mechanisms. Methodology/Principal Findings...
In humans, two glucocorticoid receptor (GR) splice variants exist: GRα and GRβ, which are identical between amino acids 1–727 then diverge. Whereas (the canonical GR) acts as a ligand-activated transcription factor, GRβ does not bind traditional agonists, lacks GRα’s transactivational activity, dominant-negative inhibitor of GRα. It has been suggested that this isoform is involved in the induction resistance asthma patients. Unfortunately, GR β-isoform detected only therefore, an animal...
Zebrafish embryos and early larvae are becoming increasingly important as models in biomedical research because of their low cost, high throughput potential a replacement for adult, higher vertebrate model species.The functional domains drug targets, other functionally proteins, often highly conserved between the zebrafish mammals.Furthermore, embryo or larva shows complex behavioural repertoire only few days after fertilization.Here, we show how studies mammals being translated to...
Background In humans, ethanol exposure during pregnancy causes a spectrum of developmental defects (fetal alcohol syndrome or FAS). Individuals vary in phenotypic expression. Zebrafish embryos develop FAS-like features after exposure. this study, we ask whether stage-specific effects can be identified the zebrafish, and if so, they allow pinpointing sensitive mechanisms. We have therefore conducted first large-scale (>1500 embryos) analysis acute, drug on zebrafish development, with large...
Abstract A general approach is proposed that allows for quantifying the relative toxic contribution of ions released from metallic nanoparticles and particles themselves, as exemplified case differently shaped zinc oxide (ZnO) (NPs) exposed to zebrafish embryos. First all, toxicity suspensions ZnO (NP (total) )—nanospheres, nanosticks, cuboidal submicron (SMPs), Zn(NO 3 ) 2 —to embryos was assessed. The observed NP assumed result combined effect present in (particle) dissolved Zn 2+ (ion) )....