A5101463552- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Biosensors and Analytical Detection
- Surface Modification and Superhydrophobicity
- RNA and protein synthesis mechanisms
- Nanoplatforms for cancer theranostics
- Protein Degradation and Inhibitors
- Luminescence and Fluorescent Materials
- Molecular Sensors and Ion Detection
- Nanomaterials and Printing Technologies
- Semiconductor materials and devices
- Mesenchymal stem cell research
- Advancements in Semiconductor Devices and Circuit Design
- Gut microbiota and health
- Advanced Photocatalysis Techniques
- Connective tissue disorders research
- Air Quality Monitoring and Forecasting
- Fluid Dynamics and Thin Films
- Nanocluster Synthesis and Applications
- Metal-Organic Frameworks: Synthesis and Applications
- Nanoparticle-Based Drug Delivery
- Advanced Nanomaterials in Catalysis
- Respiratory viral infections research
- Molecular Junctions and Nanostructures
Southwest Medical University
2021-2025
Chinese Academy of Sciences
2013-2025
Affiliated Zhongshan Hospital of Dalian University
2025
Dalian Institute of Chemical Physics
2013-2025
Zhejiang Cancer Hospital
2020-2024
Hunan University
2014-2024
University of Chinese Academy of Sciences
2013-2023
Salk Institute for Biological Studies
2020-2023
State Key Laboratory of Chemobiosensing and Chemometrics
2012-2023
University of Wisconsin Carbone Cancer Center
2023
Abstract We present a facile approach to make aptamer‐conjugated FRET (fluorescent resonance energy transfer) nanoflowers (NFs) through rolling circle replication for multiplexed cellular imaging and traceable targeted drug delivery. The NFs can exhibit multi‐fluorescence emissions by single‐wavelength excitation as result of the DNA matrix covalently incorporated with three dye molecules able perform FRET. Compared conventional nanostructure assembly, NF assembly is independent template...
Aptamers are powerful candidates for molecular imaging and targeted therapy of cancer based on such appealing features as high binding affinity, specificity, site-specific modification rapid tumor penetration. However, aptamers susceptible to plasma exonucleases in vivo. This seriously affects their vivo applications. To overcome this key limitation, we herein report the design development circular bivalent aptamers. Systematic studies reveal that cyclization can improve thermal stability,...
Abstract The host–guest chemistry of metal–organic frameworks (MOFs) has enabled the derivation numerous new functionalities. However, intrinsically chiral MOFs (CMOFs) with helical channels have not been used to realize crystalline circularly polarized luminescence (CPL) materials. Herein, enantiomeric pairs MOF crystals are reported, where achiral fluorophores adhere inner surface via biology‐like H‐bonds and hence inherit helicity host MOFs, eventually amplifying dissymmetry factor ( g...
Fluorescence resonance energy transfer (FRET) strategy has been widely applied in designing ratiometric probes for bioimaging applications. Unfortunately, FRET systems, sufficiently large spectral overlap is necessary between the donor emission and acceptor absorption, which would limit resolution of double-channel images. The through-bond (TBET) system does not need could afford wavelength difference two emissions with improved imaging higher efficiency than that classical system. It seems...
Functional nucleic acid (FNA)-based sensing systems have been developed for efficient detection of a wide range biorelated analytes by employing DNAzymes or aptamers as recognition units. However, their intracellular delivery has always concern, mainly in efficiency, kinetics, and the amount delivered FNAs. Here we report DNA dendrimer scaffold an nanocarrier to deliver FNAs conduct situ monitoring biological molecules living cells. A histidine-dependent DNAzyme anti-ATP aptamer were chosen...
This pHLIP is no flop: Functionalizing mesoporous silica nanoparticles (MSNs) with pHLIPss peptide provides a controlled-release nanoparticle drug delivery system targeting the acidic tumor microenvironment. At low pH values, inserts into cell membrane and translocates carriers cells, where cargo released by cleavage of disulfide bonds (see scheme).
DNAzymes have been widely applied as signal amplifiers for enzyme-free and highly sensitive detection of DNA. A few them also employed amplified other biomolecules via a target-triggered assembly split or mutated DNAzyme strategy. However, most these designs adopt Mg2+-dependent the catalytic unit, which suffered from low cleavage activity. Meanwhile, some with high activity are not suitable because slight modification core might results in remarkably decreased even no DNAzymes. On basis...
Disease-related biomarkers are objectively measurable molecular signatures of physiological status that can serve as disease indicators or drug targets in clinical diagnosis and therapy, thus acting a tool support personalized medicine. For example, the prostate-specific antigen (PSA) biomarker is now widely used to screen patients for prostate cancer. However, few such currently available, process identification validation prolonged complicated by inefficient methods discovery reliable...
We have developed a DNA nanoprobe for adenosine triphosphate (ATP) sensing in living cells, based on the split aptamer and triangular prism (TP). In which nucleic acid was into two fragments, stem of respectively labeled donor acceptor fluorophores that underwent fluorescence resonance energy transfer if ATP molecules were bound as target molecule to recognition module. Hence, induced self-assembly fragments thereby brought dual close proximity high (FRET) efficiency. vitro assay, an almost...
Development of efficient methods for highly sensitive and rapid screening specific oligonucleotide sequences is essential to the early diagnosis serious diseases. In this work, an aggregated cationic perylene diimide (PDI) derivative was found efficiently quench fluorescence emission a variety anionic oligonucleotide-labeled fluorophores that emit at wavelengths from visible NIR region. This broad-spectrum quencher then adopted develop multicolor biosensor via label-free approach multiplexed...
In this work, we have developed a truncated DNA aptamer, termed XQ-2d, with high affinity and specificity for pancreatic ductal adenocarcinoma (PDAC).Aptamer XQ-2d selectively binds to PL45 cells dissociation constant in the nanomolar range, as determined by its recognition of tumor mice.Moreover, shows better ratio 40 tissue sections clinical PDAC samples (82.5%) compared initial cell-SELEX selection library (5%).Therefore, can be considered promising candidate tool diagnosis treatment.
Abstract We present a facile approach to make aptamer‐conjugated FRET (fluorescent resonance energy transfer) nanoflowers (NFs) through rolling circle replication for multiplexed cellular imaging and traceable targeted drug delivery. The NFs can exhibit multi‐fluorescence emissions by single‐wavelength excitation as result of the DNA matrix covalently incorporated with three dye molecules able perform FRET. Compared conventional nanostructure assembly, NF assembly is independent template...
Aptamers and antibodies, as molecular recognition probes, play critical roles in cancer diagnosis therapy. However, their ability is based on target overexpression disease cells, not exclusivity, which can cause on-target off-tumor effects. To address the limitation, we herein report a novel strategy to develop conditional aptamer conjugate recognizes its cell surface target, but only after selective activation, determined by characteristics of microenvironment, which, our model, involve...
Abstract Attachment of lipid tails to oligonucleotides has emerged as a powerful technology in constructing cell membrane-anchorable nucleic acid-based probes. In practice, however, conventional lipid-conjugated fail distinguish among different membranes. Herein, phosphorylated oligonucleotide (DNA-lipid-P) is reported for alkaline phosphatase (ALP)-dependent membrane adhesion. the absence ALP, DNA-lipid-P with its poor hydrophobicity shows only weak interaction membrane. However, presence...
A novel viologen-based multifunctional Eu-MOF integrating photochromism, photomodulated fluorescence, and electrochromic electroluminochromic properties was investigated.