Among the vast number of recognition molecules, DNA aptamers generated from cell-SELEX exhibit unique properties for identifying cell membrane biomarkers, in particular protein receptors on cancer cells. To integrate all and computing modules within a single structure, three-dimensional (3D) DNA-based logic gate nanomachine was constructed to target overexpressed biomarkers with bispecific recognition. Thus, when Boolean operator "AND" returns true value, it is followed by an "ON" signal...

ConspectusAptamers are single-stranded DNA or RNA molecules composed of 15–80 nucleotides, obtained from a random oligonucleotide library via the systematic evolution ligands by exponential enrichment (SELEX) technology. They can bind to wide range targets with high binding affinity and specificity including metal ions, small molecules, proteins, cells, even tissues. When compared commonly used antibodies, aptamers show better thermal stability, smaller molecular weight, easier modification,...

We have developed a DNA nanoprobe for adenosine triphosphate (ATP) sensing in living cells, based on the split aptamer and triangular prism (TP). In which nucleic acid was into two fragments, stem of respectively labeled donor acceptor fluorophores that underwent fluorescence resonance energy transfer if ATP molecules were bound as target molecule to recognition module. Hence, induced self-assembly fragments thereby brought dual close proximity high (FRET) efficiency. vitro assay, an almost...

Here, we propose an efficient strategy for enzyme- and hairpin-free nucleic acid detection called entropy beacon (abbreviated as Ebeacon). Different from previously reported DNA hybridization/displacement-based strategies, Ebeacon is driven forward by increases in the of system, instead free energy released new base-pair formation. shows high sensitivity, with a limit 5 pM target buffer 50 cellular homogenate. also benefits amplification zero-background, excellent thermostability 20 °C to...

Abstract In order to maintain tissue homeostasis, cells communicate with the outside environment by receiving molecular signals, transmitting them, and responding accordingly signaling pathways. Thus, one key challenge in engineering systems involves design construction of different modules into a rationally integrated system that mimics cascade events. Herein, we DNA-based artificial uses confined microenvironment giant vesicle, derived from living cell. This consists two main components....

DNAzymes, an important type of metal ion-dependent functional nucleic acid, are widely applied in bioanalysis and biomedicine. However, the use DNAzymes practical applications has been impeded by intrinsic drawbacks natural acids, such as interferences from nuclease digestion protein binding, well undesired intermolecular interactions with other acids. On basis reciprocal chiral substrate specificity, enantiomer D-DNAzyme, L-DNAzyme, could initiate catalytic cleavage activity same achiral...

DNA nanostructures assembled on living cell membranes have become powerful research tools. Synthetic lipid been used as a membrane model to study the dynamic behavior of fluid soft bilayers, but without inherent complexity natural membranes. Herein, we report assembly and disassembly nanoprisms cell-mimicking micrometer-scale giant vesicles derived from mammalian cells. Three-dimensional with arm cholesterol anchor were efficiently localized surface. The dynamically manipulated by strand...

Abstract The novel theranostic nanosystems based on two‐photon fluorescence can achieve higher spatial resolution of deep tissue imaging for simultaneous diagnosis and therapy a variety cancers. Herein, we have designed prepared FRET‐based mesoporous silica nanoparticles (MTP–MSNs) single‐excitation multiplexed intracellular targeted cancer the first time. This nanosystem includes two constituents, containing (1) multicolor (2) cell‐targeting aptamers that act as gatekeepers MTP–MSNs. After...

Cell–cell communication plays a vital role in biological activities; particular, membrane–protein interactions are profoundly significant. In order to explore the underlying mechanism of intercellular signaling pathways, full range artificial systems have been explored. However, many them complicated and uncontrollable. Herein we designed an signal transduction system able control influx environmental ions by triggering activation synthetic transmembrane channels immobilized on giant...

Inspired by this elegant system of cellular adaptivity, we herein report the rational design a dynamic artificial adaptive able to sense and respond environmental stresses in unique sense-and-respond mode. Utilizing DNA nanotechnology, constructed an signal feedback network anchored it surface membrane model giant vesicle (GMV) protocell. Such would need both senses incoming stimuli emit response eliminate stimuli. To accomplish mechanistically, our DNA-based system, hereinafter termed...

Biomolecular condensates comprised of specific proteins and nucleic acids are now recognized as one the key organizing mechanisms in eukaryotic cells. However, roles played by acid secondary structure sequence biomolecular phase separation still not clear. Here, utilizing giant membrane vesicles (GMVs) a protocell model, we found that single-stranded DNA (ssDNA) with parallel G-quadruplex could functionally cooperate G-quadruplex-binding protein to form speckle-like puncta inside GMVs. The...

Manipulation of cell-cell interactions via cell surface engineering has potential biomedical applications in tissue and therapy. However, manipulation the comprehensive multiple intercellular remains a challenge missing elements. Herein, utilizing DNA triangular prism (TP) branched polymer (BP) as functional modules, we fabricate tunable scaffold networks on surface. The responsiveness recognition, aggregation dissociation could be modulated by aptamer-functionalized with high accuracy...

The analysis of small extracellular vesicles (sEVs) has shown clinical significance in early cancer diagnostics and considerable potential prognostic assessment therapeutic monitoring, offering possibilities for precise intervention. Despite recent diagnostic progress based on blood-derived sEVs, the inability to specifically profile multiple parameters sEVs proteins hampered advancement applications. Herein, we report an approach colorectal (CRC)-derived by using multiaptamer-triggered...

Membrane vesicles derived from live cells show great potential in biological applications due to their preserved cell membrane properties. Here, we demonstrate that cell-derived giant can be used as vectors deliver multiple therapeutic drugs and carry out combinational phototherapy for targeted cancer treatment. We efficiently encapsulated into delivered target by fusion, resulting synergistic photodynamic/photothermal therapy under light irradiation. This study highlights biomimetic drug...

Though small-molecule drugs play a crucial role in cancer treatment, intrinsic issues such as poor solubility and systematic toxicity have considerably mitigated their anticancer functions caused unwanted side effects. To achieve satisfying therapeutic efficiency, it is essential to develop innovative targeting systems for precise efficient delivery of drugs. In this work, hierarchical self-assembly strategy was applied fabricate core-shell nanoarchitecture composed DNA octahedral wireframe...

Accurate evaluation of the treatment result on X-ray images is a significant and challenging step in root canal therapy since incorrect interpretation results will hamper timely follow-up which crucial to patients' outcome. Nowadays, performed manual manner, time-consuming, subjective, error-prone. In this article, we aim automate process by leveraging advances computer vision artificial intelligence, provide an objective accurate method for assessment. A novel anatomy-guided multi-branch...

Single-molecule fluorescence in situ hybridization (smFISH) represents a promising approach for the quantitative analysis of nucleic acid biomarkers clinical tissue samples. However, low signal intensity and high background noise are complications that arise from diagnostic pathology when performed with smFISH-based RNA imaging formalin-fixed paraffin-embedded (FFPE) specimens. Moreover, associated complex procedures can produce uncertain results poor image quality. Herein, by combining...

Abstract Purpose Radical surgery, the first‐line treatment for patients with hepatocellular cancer (HCC), faces dilemma of high early recurrence rates and inability to predict effectively. We aim develop validate a multimodal model combining clinical, radiomics, pathomics features risk recurrence. Materials Methods recruited HCC who underwent radical surgery collected their preoperative clinical information, enhanced computed tomography (CT) images, whole slide images (WSI) hematoxylin eosin...

Biomimetic giant membrane vesicles, with size and lipid compositions comparable to cells, have been recognized as an attractive experimental alternative living systems. Due the similarity of their structure that body cell-derived plasma vesicles used a model for studying lipid/protein behavior membranes. However, further application biomimetic has hampered by side-effects chemical vesiculants utilization osmotic buffer. We herein develop facile strategy derive (GMVs) from mammalian cells in...