A5102824923- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Cancer-related molecular mechanisms research
- Cytokine Signaling Pathways and Interactions
- DNA and Nucleic Acid Chemistry
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- interferon and immune responses
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Immune cells in cancer
- Nanoparticle-Based Drug Delivery
- Plant Pathogens and Fungal Diseases
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Dermatology and Skin Diseases
- MicroRNA in disease regulation
- Dendrimers and Hyperbranched Polymers
- Mycotoxins in Agriculture and Food
- Inflammatory Bowel Disease
- Cancer Research and Treatments
- NF-κB Signaling Pathways
State Key Laboratory of Oncogene and Related Genes
2019-2024
Shanghai Jiao Tong University
2015-2024
Renji Hospital
2018-2024
Shanghai First People's Hospital
2020-2024
Kunming Medical University
2024
First Affiliated Hospital of Kunming Medical University
2024
Shanghai Cancer Institute
2024
Anhui Normal University
2024
Anhui Agricultural University
2023
Shanghai Clinical Research Center
2022
The ability to accurately identify and isolate cells is the cornerstone of precise disease diagnosis therapies. A single-step cell identification method based on logic analysis multiple surface markers will have unique advantages because its accuracy efficacy. Herein, using DNA aptamers for cancer biomarker recognition associative toehold activation signal integration amplification as two molecular keys, we successfully operated a cell-surface device that can perform AND Boolean biomarkers...
Abstract NF-κB is constitutively activated in psoriatic epidermis. However, how promotes keratinocyte hyperproliferation psoriasis largely unknown. Here we report that the activation triggered by inflammatory cytokines induces transcription of microRNA (miRNA) miR-31, one most dynamic miRNAs identified skin patients and mouse models. The genetic deficiency miR-31 keratinocytes inhibits their hyperproliferation, decreases acanthosis reduces disease severity Furthermore, protein phosphatase 6...
Many annotated long noncoding RNAs (lncRNAs) harbor predicted short open reading frames (sORFs), but the coding capacities of these sORFs and functions resulting micropeptides remain elusive. Here, we report that human lncRNA MIR155HG encodes a 17-amino acid micropeptide, which termed miPEP155 (P155). is highly expressed by inflamed antigen-presenting cells, leading to discovery P155 interacts with adenosine 5'-triphosphate binding domain heat shock cognate protein 70 (HSC70), chaperone...
Oncolytic viruses (OVs) have been engineered or selected for cancer cell-specific infection however, we found that following intravenous administration of vesicular stomatitis virus (VSV), tumor cell killing rapidly extends far beyond the initial sites infection. We show here first time VSV directly infects and destroys vasculature in vivo but leaves normal intact. Three-dimensional (3D) reconstruction infected tumors revealed majority mass lacks significant blood flow contrast to uninfected...
Precise and lasting immune checkpoint blockade (ICB) therapy with high objective response rate remains a significant challenge in clinical trials. We thus report the development of an aptamer-based logic computing reaction to covalently conjugate antagonizing aptamers (e.g., aPDL1 aptamer) on surface cancer cells, achieving effective sustained ICB without need for antibodies. Specifically, azides were metabolically labeled cell-surface glycoproteins as "chemical receptors", enabling...
Cytotoxic T cells initiate antitumor effects mainly through direct interactions with tumor cells. As a counter to this, can put the brakes on such T-cell activity via specific linkage between programmed death ligand 1 (PDL1) and its receptor cell protein (PD1). Bispecific inhibitors that enabled synchronous blockade of PD1 PDL1, thereby releasing activity, should significantly improve efficacy immune checkpoint (ICB) therapy. In this work, we identified DNA aptamer, Ap3, could specifically...
Abstract Dermal fibroblasts and cutaneous nerves are important players in skin diseases, while their reciprocal roles during inflammation have not been characterized. Here we identify an inflammation-induced subset of papillary that promotes aberrant neurite outgrowth psoriasiform by secreting the extracellular matrix protein tenascin-C (TNC). Single-cell analysis fibroblast lineages reveals a Tnc + with pro-axonogenesis neuro-regulation transcriptomic hallmarks. TNC overexpression boosts...
Abstract Peripherally derived regulatory T (pT reg ) cell generation requires T-cell receptor (TCR) signalling and the cytokines TGF-β1 IL-2. Here we show that TCR induces microRNA miR-31, which negatively regulates pT -cell generation. miR-31 conditional deletion results in enhanced induction of cells, decreased severity experimental autoimmune encephalomyelitis (EAE). Unexpectedly, identify Gprc5a as a direct target miR-31. is known retinoic acid-inducible protein 3, its deficiency leads...
Functionally modified aptamer conjugates are promising tools for targeted imaging or treatment of various diseases. However, broad applications molecules limited by their in vivo instability. To overcome this challenge, current strategies mostly rely on covalent chemical modification aptamers, a complicated process that requires case-by-case sequence design, multiple-step synthesis, and purification. Herein, we report modification-free strategy to enhance the stability aptamers. This simply...
Abstract Micropeptides encoded by short open reading frames (sORFs) within long noncoding RNAs (lncRNAs) are beginning to be discovered and characterized as regulators of biological pathological processes. Here, we find that lncRNA Dleu2 encodes a 17-amino-acid micropeptide, which name Dleu2-17aa, is abundantly expressed in T cells. Dleu2-17aa promotes inducible regulatory (iTreg) cell generation interacting with SMAD Family Member 3 (Smad3) enhancing its binding the Foxp3 conserved...
Research Article4 April 2017Open Access Source DataTransparent process RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease Huiyuan Zhu Key Laboratory of Cell Differentiation Apoptosis Chinese Ministry Education, Department Immunology Microbiology, Shanghai Institute Immunology, Jiao Tong University School Medicine (SJTU-SM), Shanghai, China Search for more papers by this author Fangzhou Lou Qianqian Yin Yuanyuan Gao Yang Sun Jing Bai Zhenyao Xu Zhaoyuan...
Rice bakaenii disease (RBD) is a widespread and devastating mainly caused by Fusarium fujikuroi. Pydiflumetofen (Pyd) novel succinate dehydrogenase inhibitor (SDHI) with strong inhibitory activity against F. fujikuroi, but the mechanism of resistance to Pyd has not been well studied for this pathogen. Through fungicide adaption, total 12 Pyd-resistant mutants were obtained level could be divided into three categories high (HR), moderate (MR), low (LR) factors (RF) 184.04–672.90, 12.63–42.49,...
Oligonucleotide (ON) therapeutics are emerging as a new generation of medicine with tremendous potential, but their clinical translation is hampered by inferior stability and short circulation time in the human body. Here, we report general approach to manipulating interaction between ONs albumin modulating hydrophobicity. A series DNA aptamer derivatives were designed prepared programmable synthesis an ON library gradient hydrophobic base 'F'. In vitro experiments revealed that introduction...
Abstract Pancreatic cancer is one of the most malignant tumors with highest mortality rates, and it currently lacks effective drugs. Aptamer-drug conjugates (ApDC), as a form nucleic acid drug, show great potential in therapy. However, instability acid-based drugs vivo avascularity pancreatic dense stroma have limited their application. Fortunately, VNP20009, genetically modified strain Salmonella typhimurium , which has preference for anaerobic environments, but toxic specificity, can...
Melanoma is a life-threatening cancer with limited treatments. Retinoic acid-inducible gene I (RIG-I) cytosolic pattern recognition receptor (PRR) crucial to RNA virus sensing, interferon production, and tumor suppression. Quercetin, natural flavonoid, has particularly therapeutic interests prevent treat cancer, for its pharmacological effects against oxidant, inflammation, angiogenesis. Quercetin was investigated anti-melanoma activity potential mechanisms in this study. We found that...
T helper 17 (Th17) cells play an important role in multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE). Th17 cell differentiation from naïve can be induced vitro by the cytokines transforming growth factor β1 interleukin-6. However, it remains unclear whether other regulatory factors control of cells. Mesenchymal stem (MSCs) have emerged as a promising candidate for inhibiting diseases. Despite fact that several molecules been linked to...
Biomimetic giant membrane vesicles, with size and lipid compositions comparable to cells, have been recognized as an attractive experimental alternative living systems. Due the similarity of their structure that body cell-derived plasma vesicles used a model for studying lipid/protein behavior membranes. However, further application biomimetic has hampered by side-effects chemical vesiculants utilization osmotic buffer. We herein develop facile strategy derive (GMVs) from mammalian cells in...
Psoriasis is an incurable chronic inflammatory skin disorder. The imiquimod (IMQ)-induced mouse model of psoriasis the most widely used for drug discovery and pre-clinical studies psoriasis. inflamed thickened frequently compromises quality single-cell suspensions generated from IMQ-induced lesions, which has impact on subsequent analyses by flow cytometry. This protocol details complete procedure establishment a cytometric detection immune cells in epidermis dermis. For use execution this...
Abstract Protein Phosphatase 6 down-regulation in keratinocytes is a pivotal event that amplifies the inflammatory circuits psoriasis, indicating restoration of protein phosphatase can be rational strategy for psoriasis treatment. Through phenotypic screen, we here identify L-menthol ameliorates psoriasis-like skin inflammation by increasing keratinocytes. Target identification approaches reveal an indispensable role transcription factor hairy and enhancer split 1 governing 6-upregulating...