The ability to accurately identify and isolate cells is the cornerstone of precise disease diagnosis therapies. A single-step cell identification method based on logic analysis multiple surface markers will have unique advantages because its accuracy efficacy. Herein, using DNA aptamers for cancer biomarker recognition associative toehold activation signal integration amplification as two molecular keys, we successfully operated a cell-surface device that can perform AND Boolean biomarkers...

DNA origami is a promising molecular delivery system for variety of therapeutic applications including cancer therapy, given its capability to fabricate homogeneous nanostructures whose physicochemical properties (size, shape, surface chemistry) can be precisely tailored. However, the correlation between DNA-origami design and internalization efficiency in different cell lines remains elusive. We investigated cellular uptake four (DONs) with programmed sizes shapes multiple human lines. The...

Abstract The ability to regulate membrane protein abundance offers great opportunities for developing therapeutic sites various diseases. Herein, we describe a platform the targeted degradation of membrane‐associated proteins using bispecific aptamer chimeras that bind both cell‐surface lysosome‐shuttling receptor (IGFIIR) and membrane‐bound interest. We demonstrate can efficiently quickly shuttle therapeutically relevant Met PTK‐7 lysosomes degrade them through lysosomal machinery....

Rapid tests for pathogen identification and spread assessment are critical infectious disease control prevention. The of viral outbreaks requires a nucleic acid diagnostic test that is sensitive simple delivers fast reliable results. Here, we report one-pot direct reverse transcript loop-mediated isothermal amplification (RT-LAMP) assay SARS-CoV-2 based on lateral flow in clinical samples. entire contiguous sample-to-answer workflow takes less than 40 min from swab sample to result without...

Abstract Molecular computing is an emerging paradigm that plays essential role in data storage, bio-computation, and clinical diagnosis with the future trends of more efficient scheme, higher modularity scaled-up circuity stronger tolerance corrupted inputs a complex environment. Towards these goals, we construct spatially localized, DNA integrated circuits-based classifier (DNA IC-CLA) can perform neuromorphic architecture-based computation at molecular level for medical diagnosis. The...

Gold magnetic nanoparticles (GoldMag) have attracted great attention due to their unique physical and chemical performances combining those of individual Fe3O4 Au nanoparticles. Coating GoldMag with polymers not only increases the stability composite particles suspended in buffer but also plays a key role for establishing point-of-care optical tests clinically relevant biomolecules. In present paper, poly(4-styrenesulfonic acid-co-maleic acid) (PSS-MA), negatively charged polyelectrolyte...

Abstract A single‐step method for isolation of specific cells based on multiple surface markers will have unique advantages because its scalability, efficacy, and mildness. Herein, we developed multi‐aptamer‐mediated proximity ligation live cell membranes that leverages a multi‐receptor co‐recognition design enhanced specificity, as well robust in situ signal amplification improved sensitivity isolation. We demonstrated the promising efficacy our differentiating tumor subtypes both mixtures...

Terahertz (THz) waves show nontrivial interactions with living systems, but the underlying molecular mechanisms have yet to be explored. Here, we employ DNA origami as a model system study between THz and structures. We find that 3-min illumination (35.2 THz) can drive unwinding of duplexes at ∼10 °C below their melting point. Computational reveals wave resonate vibration bases, provoking hydrogen bond breaking. The cooperation thermal nonthermal effects allows unfolding undesired secondary...

Abstract Active tumor penetration has been recently recognized as a promising strategy to resolve the limitation of nanomedicine in terms penetration, but it is challenging develop active transporting nanocarriers. Here, an ultrasonic cavitation‐assisted and acid‐activatable liposome for broad range tumors reported. The (size charge dual‐conversional gemcitabine prodrug‐integrated liposomal nanodroplet (SCGLN)) overcomes tight blood vessel walls with aid cavitation. SCGLN subsequently...

The novel coupling method based on the hyphenation of immunoreaction with ICP-MS was developed and applied to determination thyroid-stimulating hormone (TSH) in human serum. In this system, TSH firstly captured by anti-TSH monoclonal antibodies immobilized a solid support. Biotinylated Eu3+-labelled streptavidin were then added form complex, antibody–TSH–biotinylated antibody–Eu3+-labelled streptavidin. After unbound reactants washed away, Eu3+ bound complex extracted 1% HNO3 measured...

Abstract Background: An inductively coupled plasma mass spectrometry (ICP-MS)-based immunoassay has been proposed independently by Baranov et al. (Anal Chem 2002;74:1629–36) and our group, but the applicability of this method for multianalyte analysis in clinical samples not fully illustrated. We developed a dual-label simultaneous determination α-fetoprotein (AFP) free β-human chorionic gonadotropin (hCGβ) human serum. Methods: Monoclonal antibodies immobilized on microtiter plates captured...

One‐dimensional (1D) porous materials have shown great potential for gas storage and separation, sensing, energy storage, conversion. However, the controlled approach preparation of 1D materials, especially organic still remains a challenge due to poor dispersibility solution processability materials. Here, carbon nanotube (CNT) templated conjugated microporous polymers (CMPs) are prepared using layer‐by‐layer method. As‐prepared CMPs possess high specific surface areas up 623 m 2 g −1...

Reactive oxygen species (ROS)-responsive nanocapsules for cancer drug delivery were engineered from green tea polyphenol–metal networks.

Rapid and accurate classification of the etiology for acute respiratory illness not only helps establish timely therapeutic plans but also prevents inappropriate use antibiotics. Host gene expression patterns in peripheral blood can discriminate bacterial from viral causes infection (ARI) suffer long turnaround time, as well high cost resulting measurement methods microarrays next-generation sequencing. Here, we developed an automated DNA computing–based platform that implement silico...

To ensure that anticancer drugs could be delivered to tumor region and rapidly escape from endo/lysosomes, YPSMA-1-modified pH-sensitive polymeric micelles were designed fabricated for targeted therapy of tumors.

To achieve efficient resource management on a graphics processing unit (GPU), there is demand to develop framework for scheduling virtualized resources in cloud gaming. In this article, we propose VGRIS, GPU isolation and A set of application programming interfaces (APIs) provided so that variety algorithms can be implemented within the without modifying itself. Three are by APIs VGRIS. Experimental results show VGRIS effectively schedule among various workloads.

A methoxy‐poly(ethylene glycol)‐ block ‐poly(acrylamide‐ co ‐acrylonitrile) (mPEG‐ b ‐P(AAm‐ ‐AN)) amphiphilic copolymer exhibiting upper critical solution temperature (UCST) behavior is synthesized, and micelles from this are fabricated. It found that the thermal responses of these tunable through balancing hydrophobic/hydrophilic blocks in copolymer. The size doxorubicin (DOX)‐loaded dependent on hydrophobic interaction as well hydrogen bonding between polymer drug molecules. As a proof...

Single nucleotide polymorphisms (SNPs) are closely related to genetic diseases, but current SNP detection methods, such as DNA microarrays that include tedious procedures and expensive, sophisticated instruments, unable perform rapid SNPs in clinical practice, especially for those multiple diseases. In this study, we report a sensitive, low cost, easy-to-use point-of-care testing (POCT) system formed by combining amplification refractory mutation (ARMS) polymerase chain reaction with gold...

As the virtualization technology for GPUs matures, cloud gaming has become an emerging application among services. In addition to poor default mechanisms of GPU resource sharing, performance games is inevitably undermined by various runtime uncertainties such as rendering complex game scenarios. The question how handle sharing remains unanswered. To address this challenge, we propose vGASA, a virtualized adaptive scheduling algorithm in gaming. vGASA interposes algorithms graphics API...