A5026940690- Microbial Natural Products and Biosynthesis
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Infectious Diseases and Mycology
- Plant-Microbe Interactions and Immunity
- Microbial Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Antifungal resistance and susceptibility
- Bioactive Compounds and Antitumor Agents
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Tuberculosis Research and Epidemiology
- Antimicrobial Peptides and Activities
- Fungal Biology and Applications
- Bone Metabolism and Diseases
- Quinazolinone synthesis and applications
- Fungal Plant Pathogen Control
- Sphingolipid Metabolism and Signaling
- Metabolism and Genetic Disorders
- Heterotopic Ossification and Related Conditions
- Insects and Parasite Interactions
Kitasato University
2012-2024
University of Montana
2014-2016
University of California, San Diego
2016
Cambridge University Press
2009
New York University Press
2009
Tokyo University of Science
2008
Kitasato Institute Hospital
2007
ABSTRACT Potatoes are cultivated in southwest Greenland without the use of pesticides and with limited crop rotation. Despite fact that plant-pathogenic fungi present, no severe-disease outbreaks have yet been observed. In this report, we document a potato soil at Inneruulalik southern is suppressive against Rhizoctonia solani Ag3 uncover antifungal mechanism highly potent biocontrol bacterium, Pseudomonas fluorescens In5, isolated from soil. A combination molecular genetics, genomics,...
New fungal metabolites, designated quinadolines A (1) and B (2), were isolated from culture broth of Aspergillus sp. FKI-1746, their structures elucidated by NMR spectroscopy. The complete relative absolute stereochemistry 2 was determined X-ray crystallography amino acid analysis using a chiral column. Quinadolines moderately inhibited lipid droplet synthesis in mouse macrophages.
Staphyloxanthin, a yellow pigment produced by methicillin-resistant Staphylococcus aureus (MRSA), is virulent factor escaping from the host immune system. A new screening method for inhibitors of staphyloxanthin production MRSA was established using paper disks. By this method, were selected natural product library (ca. 300) and actinomycete culture broths 1000). From library, four known lipid metabolism, cerulenin, dihydrobisvertinol, xanthohumol zaragozic acid, found to inhibit production;...
complex (MAC) is a serious disease that mainly caused by infection with the non-tuberculous mycobacteria (NTM),
New rugulosins B (2) and C (3) were isolated together with known rugulosin (renamed A in this paper, 1) from whole culture of Penicillium radicum FKI-3765-2, their structures elucidated by NMR spectroscopy. Rugulosins a homodimer the same anthraquinone moieties, whereas was heterodimer analogous moieties. to showed antimicrobial activity against methicillin-resistant Staphylococcus aureus.
The nonantibiotic small molecule cyslabdan, a labdan-type diterpene produced by Streptomyces sp. K04-0144, markedly potentiated the activity of β-lactam drug imipenem against methicillin-resistant Staphylococcus aureus (MRSA). To study mechanism action proteins that bind to cyslabdan were investigated in an MRSA lysate, which led identification FemA, is involved synthesis pentaglycine interpeptide bridge peptidoglycan MRSA. Furthermore, binding assay FemB and FemX with function similar FemA...
Mycobacterium avium complex (MAC) is a serious disease mainly caused by M. and M.intracellulare. Although the incidence of MAC infection increasing worldwide, only few agents are clinically used, their therapeutic effects limited. Therefore, new anti-MAC needed. Approximately 6600 microbial samples were screened for anti-mycobacterial that inhibit growth both intracellulare, two culture broths derived from marine actinomycete strains OPMA1245 OPMA1730 had strong activity. Nosiheptide (1) was...
Abstract Lariatin A, an 18-residue lasso peptide encoded by the five-gene cluster larABCDE , displays potent and selective anti-mycobacterial activity. The structural feature is N -terminal macrolactam ring, through which C passed to form rigid lariat-protoknot structure. In present study, we established a convergent expression system strategy in larA mutant gene-carrying plasmids were transformed into -deficient Rhodococcus jostii generated 36 lariatin variants of precursor protein LarA...
Among four mycobacteria, Mycobacterium avium, M. intracellulare, bovis BCG and Mycobacteroides (My.) abscessus, we established a silkworm infection assay with My. abscessus. When silkworms (fifth-instar larvae, n = 5) were infected through the hemolymph abscessus (7.5 × 107 CFU/larva) bred at 37 °C, they all died around 40 h after injection. Under conditions, clarithromycin amikacin, clinically used antimicrobial agents, exhibited therapeutic effects in dose-dependent manner. Furthermore,...
[structure: see text] New isobisvertinol and known bisvertinol were isolated from the culture broth of Aspergillus sp. FKI-1746. Isobisvertinol with two alkenyl side chains extending in same direction inhibited lipid droplet accumulation macrophages, whereas those reverse had almost no effect on accumulation.
A new fungal metabolite designated calpinactam (1) was isolated from the culture broth of Mortierella alpina FKI-4905, and its structure elucidated by spectroscopic analyses including NMR experiments. Calpinactam found to be a hexapeptide with caprolactam ring at C-terminal. Its absolute stereochemistry determined amino acid analysis total synthesis. selectively inhibited growth mycobacteria among various microorganisms. The MIC values against Mycobacterium smegmatis M. tuberculosis were...