A5008160379- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Computational Drug Discovery Methods
- Mass Spectrometry Techniques and Applications
- Higher Education and Teaching Methods
- Protein Structure and Dynamics
- Microbial Metabolism and Applications
- Genomics and Phylogenetic Studies
- Neuroscience and Neural Engineering
- Neurotransmitter Receptor Influence on Behavior
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Innovative Educational Techniques
- Marine Sponges and Natural Products
- Protein Degradation and Inhibitors
- Histone Deacetylase Inhibitors Research
- Muscle activation and electromyography studies
- Fluid Dynamics Simulations and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Ship Hydrodynamics and Maneuverability
- Plasmonic and Surface Plasmon Research
- Wave and Wind Energy Systems
- Phytochemical compounds biological activities
ShanghaiTech University
2017-2025
Guangdong Provincial Health Commission
2025
Soochow University
2024
Wuhan University of Science and Technology
2024
Beijing VDJBio (China)
2024
China Pharmaceutical University
2021-2023
Chinese Academy of Sciences
2017-2022
University of Chinese Academy of Sciences
2017-2022
Beijing Institute of Big Data Research
2022
Kunming Medical University
2013-2018
Drugs frequently require interactions with multiple targets—via a process known as polypharmacology—to achieve their therapeutic actions. Currently, drugs targeting several serotonin receptors, including the 5-HT2C receptor, are useful for treating obesity, drug abuse, and schizophrenia. The competing challenges of developing selective receptor ligands or creating defined polypharmacological profile, especially aimed at G protein-coupled receptors (GPCRs), remain extremely difficult. Here,...
Severe fever with thrombocytopenia syndrome is an emerging infectious disease caused by a novel bunyavirus (SFTSV). Lack of vaccines and inadequate therapeutic treatments have made the spread virus global concern. Viral nucleocapsid protein (N) essential for its transcription replication. Here, we present crystal structures N from SFTSV homologs Buenaventura (BUE) Granada (GRA) viruses. The reveal that phleboviral folds into compact core domain extended N-terminal arm mediates...
Engineering of GPCR constructs with improved thermostability is a key for successful structural and biochemical studies this transmembrane protein family, targeted by 40% all therapeutic drugs. Here we introduce comprehensive computational approach to effective prediction stabilizing mutations in GPCRs, named CompoMug, which employs sequence-based analysis, information, derived machine learning predictor. Tested experimentally on the serotonin 5-HT2C receptor target, CompoMug predictions...
Paired homologs of γ-butyrolactone (GBL) biosynthesis gene afsA and GBL receptor arpA are located at different positions in genome Streptomyces hygroscopicus 5008. Inactivation dramatically decreased validamycin, an important anti-fungal antibiotic a critical substrate for antidiabetic drug synthesis, the deletion increased validamycin production by 26% (ΔshbR1) 20% (ΔshbR3). By double deletion, ΔshbR1/R3 mutant showed higher transcriptional levels adpA-H (the S. ortholog global regulatory...
ABSTRACT The assessment of human papillomavirus (HPV) genotype distribution could inform targeted cervical cancer prevention strategies. epidemiology HPV genotypes in terms age and lesions Fujian Province, China has not been well described. This 9‐year retrospective study aimed to delineate the prevalence pattern trend among a large‐scale community‐based population. Deidentified data were retrieved from national screening program China. We included eligible women aged 35–65 years who...
Natural product screening programs have uncovered molecules from diverse natural sources with various biological activities and unique structures. However, much is yet underexplored additional information hidden in these exceptional collections. We applied untargeted mass spectrometry approaches to capture the chemical space dispersal patterns of metabolites an in-house library marine cyanobacterial algal Remarkably, 86% metabolomics signals detected were not found other available datasets...
High-throughput identification of GPCR modulators through affinity MS screening.
Subtype selectivity and functional bias are vital in current drug discovery for G protein-coupled receptors (GPCRs) as selective biased ligands expected to yield leads with optimal on-target benefits minimal side-effects. However, structure-based design medicinal chemistry exploration remain challenging part because of highly conserved binding pockets within subfamilies. Herein, we present an affinity mass spectrometry approach screening herbal extracts identify active a GPCR, the 5-HT2C...
G protein-coupled receptors (GPCRs) constitute the largest human protein family with over 800 members, which are implicated in many important medical conditions. Serotonin belong to aminergic GPCR subfamily and play roles physiological psychological activities. Structural biology studies have revealed structures of GPCRs atomic details provide basis for identification investigation potential ligands, interact modulate receptors. Here, an integrative approach combining a focused...
The α-glucosidase inhibitor acarbose is commercially produced by
We study the mechanism of polarization-selective transmission in double-layer gratings. For TM polarized light, coupling between surface plasmon waveguide modes slits two metal layers causes most energy to pass TE there is no effect, and photoresist reflected back because cutoff modes. In view characteristics modes, a new polarizer with an undercut profile proposed achieve better performance by improving efficiency. At pitch 200nm, transmissions can be over 70% visible spectrum 80%...
The class B family of G-protein-coupled receptors (GPCRs) has long been a paradigm for peptide hormone recognition and signal transduction. One GPCR, the glucagon-like peptide-1 receptor (GLP-1R), considered as an anti-diabetes drug target there are several peptidic drugs available treatment this overwhelming disease. previously determined structures inactive GLP-1R in complex with two negative allosteric modulators include ten thermal-stabilizing mutations that were selected from total 98...
With continuous breakthrough of key technologies, common emerging cross and subversive which have brought profound changes to politics, economy, society, culture, environment. Under above-mentioned background, further exert the guiding role technology foresight in planning formulation, study supporting management, is important for academic research, enterprise practice government decision-making. This article can be divided into two parts, literature review part mainly includes published...
Acetate is a central metabolite that plays key role in almost all organisms, and acetate channels are often essential for their survival. Recently solved structures of the channel Succinate-Acetate Permease (SatP) provide an atomic view its closed state. However, open state channel, residue conformational changes trigger to open, free energy barrier transportation remain elusive. To address these questions, we performed microsecond time scale molecular dynamics (MD) simulations umbrella...
The combination of nanodisc and affinity LC/MS techniques potentiates the high-throughput screening against GPCRs.
With the increasing demand of floating structures in offshore, coastal and marine renewable energy engineering, interaction between mooring system structure becomes more important. In this paper, motion responses a semi-submersible platform with under regular wave conditions are investigated numerically by viscous flow solver naoe-FOAM-SJTU based on open source toolbox OpenFOAM. Influence is evaluated two different ways. Investigations covered for analysis methods adopted solving lines...
A musculoskeletal model that allows to simulate the tremor of wrist joint with three degrees freedom (DoFs) is developed. The includes five muscles, extensor carpi radialis brevis, longus, ulnaris, flexor and ulnaris. Simulation generation based on 3-DoF performed. disorder can be generated in two directions: flexion-extension radia-ulnar deviation. Accordingly, experiment conducted healthy subjects verify feasibility artificial via functional electrical stimulation (FES). results have shown...