A5040694365- Receptor Mechanisms and Signaling
- Hydrocarbon exploration and reservoir analysis
- Biochemical and Molecular Research
- Hedgehog Signaling Pathway Studies
- Geological and Geochemical Analysis
- Urban Green Space and Health
- Diverticular Disease and Complications
- earthquake and tectonic studies
- Atmospheric and Environmental Gas Dynamics
- Analytical Chemistry and Sensors
- Cancer-related gene regulation
- Geological and Tectonic Studies in Latin America
- Geochemistry and Geologic Mapping
- Neuropeptides and Animal Physiology
- HIV/AIDS drug development and treatment
- Methane Hydrates and Related Phenomena
- Advanced Proteomics Techniques and Applications
- Advanced Chemical Sensor Technologies
- Land Use and Ecosystem Services
- Mass Spectrometry Techniques and Applications
- Underwater Acoustics Research
- Fire Detection and Safety Systems
- Water Quality Monitoring and Analysis
- Photochromic and Fluorescence Chemistry
- Autoimmune and Inflammatory Disorders
Nanjing University of Chinese Medicine
2024
Beijing Forestry University
2024
Dalian University of Technology
2024
ShanghaiTech University
2017-2022
University of Chinese Academy of Sciences
2017-2021
Xi'an Shiyou University
2021
Chinese Academy of Sciences
2016-2020
Institute of Deep-Sea Science and Engineering
2017-2020
Shanghai Institutes for Biological Sciences
2017-2019
Wuhan Institute of Technology
2018
Drugs frequently require interactions with multiple targets—via a process known as polypharmacology—to achieve their therapeutic actions. Currently, drugs targeting several serotonin receptors, including the 5-HT2C receptor, are useful for treating obesity, drug abuse, and schizophrenia. The competing challenges of developing selective receptor ligands or creating defined polypharmacological profile, especially aimed at G protein-coupled receptors (GPCRs), remain extremely difficult. Here,...
Coupled magmatic and tectonic activity plays an important role in high-temperature hydrothermal circulation at mid-ocean ridges. The patterns for such systems have been elucidated by microearthquakes geochemical data over a broad spectrum of spreading rates, but not generally available ultra-slow Here we report new geophysical fluid active venting Dragon Horn area (49.7°E) on the Southwest Indian Ridge. Twin detachment faults penetrating to depth 13 ± 2 km below seafloor were identified...
Significance Recent success of T cell-based cancer immunotherapies highlights the importance further understanding molecular mechanisms in regulation cell responsiveness. Here, we performed a genome-wide CRISPR screen to identify genes that regulate activation upon anti-T receptor (TCR) stimulation. Our confirmed many known regulators proximal signaling. Moreover, identified previously uncharacterized gene named FAM49B, which acts as negative regulator activation. study suggests screening is...
Abstract The Smoothened receptor (SMO) belongs to the Class Frizzled of G protein-coupled (GPCR) superfamily, constituting a key component Hedgehog signalling pathway. Here we report crystal structure multi-domain human SMO, bound and stabilized by designed tool ligand TC114, using an X-ray free-electron laser source at 2.9 Å. reveals precise arrangement three distinct domains: seven-transmembrane helices domain (TMD), hinge (HD) intact extracellular cysteine-rich (CRD). This architecture...
Asparagine synthetase (ASNS) and CTP synthase (CTPS) are two metabolic enzymes crucial for glutamine homeostasis. A genome-wide screening in Saccharomyces cerevisiae reveal that both ASNS CTPS form filamentous structures termed cytoophidia. Although cytoophidia were well documented recent years, the filamentation of is less studied. Using budding yeast as a model system, here we confirm proteins, Asn1 Asn2, capable forming diauxic stationary phases. We find glucose deprivation induces...
Data-independent acquisition mass spectrometry (DIA-MS) is a powerful technique that enables relatively deep proteomic profiling with superior quantification reproducibility. DIA data mining predominantly relies on spectral library of sufficient proteome coverage that, in most cases, built data-dependent acquisition-based analysis the same sample. To expand for pre-determined protein family, we report herein construction hybrid supplements experiment-derived family-targeted virtual predicted...
Urban green spaces are crucial for improving the quality of life city residents because they provide numerous health advantages and foster overall wellness. However, previous research has concentrated only on individual goals overlooked inherent contradictions that arise when many objectives considered. This study devised a transdisciplinary framework to link optimization plant community characteristics with multiple objectives. In Beijing, we selected 22 urban utilized non-dominated sorting...
We present herein a novel dissection-then-evolution strategy for ligand optimization.
China's 7000 m manned submersible JIAOLONG carried out an exploration cruise at the Mariana Trench from June to July 2016. The completed nine dives on north and south area of depth 5500 6700 m, investigate geological, biological chemical characteristics in hadal area. During cruise, deployed a gas-tight serial sampler collect water near sea bottom regularly. Five days later, sub located another dive retrieved it successfully same location, which is first time that scientists engineers...
We unveiled an underside binding site on smoothened receptor (SMO) by a colocalization strategy using two structurally complementary photoaffinity probes derived from known ligand Allo-1. Docking study and structural dissection identified key interactions within the site, including hydrogen bonding, π–π interactions, hydrophobic between Allo-1 its contacting residues. Taken together, our results reveal molecular base of provide basis for design new-generation ligands to overcome drug resistance.
Abstract Data-independent acquisition mass spectrometry (DIA-MS) is a rapidly evolving technique that enables relatively deep proteomic profiling with superior quantification reproducibility. DIA data mining predominantly relies on spectral library of sufficient proteome coverage that, in most cases, built data-dependent acquisition-based analysis the same sample. To expand for pre-determined protein family, we report herein construction hybrid supplements experiment-derived family-targeted...
TC4 components manufactured by laser directed energy deposition often suffer from problems such as coarse grains and anisotropy, which limit their application in the field of load-bearing. To address these challenges, a synchronous-hammer-forging-assisted additive manufacturing method was proposed its effects on macro morphology, microstructure, mechanical properties Ti-6Al-4V were systematically investigated. The results indicate that plastic deformation 19.1% formed titanium alloy...
Abstract A pumping unit with incomplete gear and rack reversal was designed for smooth motion low energy consumption. This paper first describes the working principle of unit. The kinematical equation is established on basis analyzing kinematic relationship between tooth meshing area, normal area final rack. curve obtained by ADAMS simulation. simulation results show that theoretical model correct. By comparing conventional unit, it can be seen that: has a movement up-down strokes,...