A5018617348- Advanced Proteomics Techniques and Applications
- Alzheimer's disease research and treatments
- Parkinson's Disease Mechanisms and Treatments
- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Metabolomics and Mass Spectrometry Studies
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- Mitochondrial Function and Pathology
- Neuroscience and Neuropharmacology Research
- Histone Deacetylase Inhibitors Research
- Tryptophan and brain disorders
- interferon and immune responses
- RNA modifications and cancer
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- RNA Research and Splicing
- Peptidase Inhibition and Analysis
- Geophysical and Geoelectrical Methods
- Heat shock proteins research
- RNA regulation and disease
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
Shanghai Institute of Organic Chemistry
2015-2025
Chinese Academy of Sciences
2015-2025
Second Affiliated Hospital of Dalian Medical University
2024
Chinese PLA General Hospital
2024
Dalian Medical University
2020-2024
Geological Exploration Technology Institute of Jiangsu Province
2023-2024
Zhengzhou University
2023
University of Chinese Academy of Sciences
2018-2023
Jinan Stomatological Hospital
2023
Ocean University of China
2022-2023
Multiple sclerosis (MS), a common neurodegenerative disease of the CNS, is characterized by loss oligodendrocytes and demyelination. Tumor necrosis factor α (TNF-α), proinflammatory cytokine implicated in MS, can activate necroptosis, necrotic cell death pathway regulated RIPK1 RIPK3 under caspase-8-deficient conditions. Here, we demonstrate defective caspase-8 activation, as well activation RIPK1, RIPK3, MLKL, hallmark mediators cortical lesions human MS pathological samples. Furthermore,...
Although glucose uniquely stimulates proinsulin biosynthesis in β cells, surprisingly little is known of the underlying mechanism(s). Here, we demonstrate that activates unfolded protein response transducer inositol-requiring enzyme 1 alpha (IRE1α) to initiate X-box-binding (Xbp1) mRNA splicing adult primary cells. Using sequencing (mRNA-Seq), show unconventional Xbp1 required increase and decrease expression several hundred mRNAs encoding functions expand secretory capacity for increased...
Biomimetic flexible electronics for E-skin have received increasing attention, due to their ability sense various movements. However, the development of smart skin-mimic material remains a challenge. Here, simple and effective approach is reported fabricate super-tough, stretchable, self-healing conductive hydrogel consisting polyvinyl alcohol (PVA), Ti3 C2 Tx MXene nanosheets, polypyrrole (PPy) (PMP hydrogel). The nanosheets Fe3+ serve as multifunctional cross-linkers stress transfer...
A plethora of software suites and multiple classes spectral libraries have been developed to enhance the depth robustness data-independent acquisition (DIA) data processing. However, how combination a DIA tool library impacts outcome proteomics phosphoproteomics analysis has rarely investigated using benchmark that mimics biological complexity. In this study, we create sets simulating regulation thousands proteins in complex background, which are collected on both an Orbitrap timsTOF...
Aspirin is a potent lysine acetylation inducer, but its impact on ubiquitination and ubiquitination-directed protein degradation unclear. Herein, we develop the reversed-pulsed-SILAC strategy to systematically profile degradome in response aspirin. By integrating degradome, acetylome, ubiquitinome analyses, show that aspirin impairs proteasome activity inhibit proteasomal degradation, rather than directly suppressing ubiquitination. Interestingly, increases lysosomal degradation-implicated...
MS-based proteomics has emerged as a powerful tool in biological studies. The shotgun strategy, which proteolytic peptides are analyzed data-dependent mode, enables detection of the most comprehensive proteome (>10 000 proteins from whole-cell lysate). quantitative uses stable isotopes or label-free method to measure relative protein abundance. isotope labeling strategies more precise and accurate compared methods, but procedures complicated expensive, sample number types also limited....
Epigenetic alteration has been implicated in aging. However, the mechanism by which epigenetic change impacts aging remains to be understood. H3K27me3, a highly conserved histone modification signifying transcriptional repression, is marked and maintained Polycomb Repressive Complexes (PRCs). Here, we explore age-modulated increase of H3K27me3 adult lifespan. Using
Hexokinase II (HK2), a key enzyme involved in glucose metabolism, is regulated by growth factor signaling and required for initiation maintenance of tumors. Here we show that metabolic stress triggered perturbation receptor tyrosine kinase FLT3 non–acute myeloid leukemia cells sensitizes cancer to autophagy inhibition leads excessive activation chaperone-mediated (CMA). Our data demonstrate an important sensor cellular nutritional state elucidate the role molecular mechanism CMA regulation...
Abstract Background Bone tissue engineering is a new concept bringing hope for the repair of large bone defects, which remains major clinical challenge. The formation vascularized key engineering. Growth specialized blood vessels termed type H associated with formation. In vivo and in vitro studies have shown that low level laser therapy (LLLT) promotes angiogenesis, fracture healing, osteogenic differentiation stem cells by increasing reactive oxygen species (ROS). However, whether LLLT can...
Abstract α-Synuclein (α-Syn) can form different fibril strains with distinct polymorphs and neuropathologies, which is associated the clinicopathological variability in synucleinopathies. How α-syn are produced selected under disease conditions remains poorly understood. In this study, we show that hereditary mutation G51D induces to a strain vitro. The cryogenic electron microscopy (cryo-EM) structure of was determined at 2.96 Å resolution. displays relatively small extended serpentine fold...
The identification of differentially regulated proteins in animal models psychiatric diseases is essential for a comprehensive analysis associated psychopathological processes. Mass spectrometry the most relevant method analyzing differences protein expression tissue and body fluid proteomes. However, standardization sample handling sample-to-sample variability are problematic. Stable isotope metabolic labeling proteome represents gold standard quantitative mass analysis. simultaneous...
Depression and anxiety disorders affect a great number of people worldwide. Whereas singular factors have been associated with the pathogenesis psychiatric disorders, growing evidence emphasizes significance dysfunctional neural circuits signaling pathways. Hence, systems biology approach is required to get better understanding phenotypes such as depression anxiety. Furthermore, availability biomarkers for these critical improved diagnosis monitoring treatment response. In present study,...
Significance Fragile X syndrome (FXS) is a frequent mental disorder characterized by intellectual disability and other symptoms including autism. The disease gene-encoded protein FMRP regulates activity-dependent translation of large number mRNAs in neurons. We used quantitative mass spectrometry to systematically compare expression neocortical synaptic fractions between Fmr1 (fragile retardation 1) knockout (KO) wild-type mice during adolescence adulthood. discovered an upregulation...
Significance A single base substitution in one allele of the PIK3CA gene (encoding catalytic subunit p110α PI3K) a human breast epithelial cell induces expression profile that closely resembles basal cancer. The mutation also causes extensive remodeling signatures are not known to be connected activity PI3K. data show cancer-specific gain function PI3K has an unexpectedly deep and broad impact on phenotypic properties cell.
Biocompatible reactions are powerful tools to probe protein functions in their native environment. Due the difficulty of penetrating live-cell membrane and complex intracellular environment, biocompatible inside live cells challenging, especially at subcellular level with spatial resolution. Here we report first photocatalytic azide conjugation reaction achieve mitochondria-selective proteins labeling. The organic dyes acridine orange, fluorescein, rhodamine 123 were developed as...