A5085141843- Cell death mechanisms and regulation
- Autophagy in Disease and Therapy
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- NF-κB Signaling Pathways
- Alzheimer's disease research and treatments
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Inflammasome and immune disorders
- Immune Response and Inflammation
- Cellular transport and secretion
- S100 Proteins and Annexins
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Hippo pathway signaling and YAP/TAZ
- Neuroscience and Neuropharmacology Research
- Calcium signaling and nucleotide metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Nuclear Structure and Function
- PARP inhibition in cancer therapy
- Cerebral Palsy and Movement Disorders
Shanghai Institute of Organic Chemistry
2016-2025
Chinese Academy of Sciences
2016-2025
Affiliated Hospital of Guizhou Medical University
2025
Gansu University of Traditional Chinese Medicine
2025
Third Affiliated Hospital of Zhengzhou University
2014-2024
University of Chinese Academy of Sciences
2024
China Medical University
2024
Harvard University
2013-2023
Nanfang Hospital
2022
Nanfang College Guangzhou
2022
Most protein phosphatases have little intrinsic substrate specificity, making selective pharmacological inhibition of specific dephosphorylation reactions a challenging problem. In screen for small molecules that protect cells from endoplasmic reticulum (ER) stress, we identified salubrinal, inhibitor cellular complexes dephosphorylate eukaryotic translation initiation factor 2 subunit alpha (eIF2alpha). Salubrinal also blocks eIF2alpha mediated by herpes simplex virus and inhibits viral...
Calpains and caspases are two cysteine protease families that play important roles in regulating pathological cell death. Here, we report m-calpain may be responsible for cleaving procaspase-12, a caspase localized the ER, to generate active caspase-12. In addition, calpain loop region Bcl-xL and, therefore, turning an antiapoptotic molecule into proapoptotic molecule. We propose disturbance intracellular calcium storage as result of ischemic injury or amyloid β peptide cytotoxicity induce...
We examined the expression, activation, and cellular localization of caspase-3 (CPP32) using immunohistochemistry, immunoblots, cleavage fluorogenic substrate N-benzyloxycarbonyl-Asp-Glu-Val-Asp-7-amino-4-trifluoromethyl coumarin (zDEVD-afc) in adult mouse brain after temporary (2 hr) middle cerebral artery occlusion produced by filament insertion into carotid artery. Immunoreactive caspase-3p32 but not its product caspase-3p20 was constitutively expressed neurons throughout most prominent...
The interleukin 1β converting enzyme (ICE) family plays a pivotal role in programmed cell death and has been implicated stroke neurodegenerative diseases. During reperfusion after filamentous middle cerebral artery occlusion, ICE-like cleavage products tissue immunoreactive (IL-1β) levels increased ischemic mouse brain. Ischemic injury decreased intracerebroventricular injections of protease inhibitors, N -benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD.FMK),...
Louise Bergeron, Gloria I. Perez, Glen Macdonald, Lianfa Shi, Yi Sun, Andrea Jurisicova, Sue Varmuza, Keith E. Latham, Jodi A. Flaws, Jessica C.M. Salter, Hideaki Hara, Michael Moskowitz, En Li, Arnold Greenberg, Jonathan L. Tilly, and Junying Yuan Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 USA; The Vincent Center for Reproductive Obstetrics Gynecology, General Hospital/Harvard 02114 Manitoba Institute Cancer Treatment Research Foundation, University...