A5001442165- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Diabetes and associated disorders
- Diabetes Management and Research
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes Treatment and Management
- Lipid metabolism and biosynthesis
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Nutritional Studies and Diet
- Metabolomics and Mass Spectrometry Studies
- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Cannabis and Cannabinoid Research
- Regulation of Appetite and Obesity
- Pancreatitis Pathology and Treatment
- Cellular transport and secretion
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- Erythrocyte Function and Pathophysiology
Boston University
2016-2025
Boston Medical Center
2002-2021
University School
2020
Adler
2017
Cardiovascular Research Center
2016
University of Pennsylvania
1984-2015
Hospital General Universitario Gregorio Marañón
2015
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
1983-2015
University Medical Center
1990-2014
Johns Hopkins Medicine
2010
One of the unique features beta-cells is their relatively low expression many antioxidant enzymes. This could render susceptible to oxidative damage but may also provide a system that sensitive reactive oxygen species as signals. In isolated mouse islets and INS-1(832/13) cells, glucose increases intracellular accumulation H2O2. both models, insulin secretion be stimulated by provision either exogenous H2O2 or diethyl maleate, which raises levels. Provision scavengers, including cell...
hypothesis proposing a role for acyl-CoA esters in nutrient-induced insulin release (Prentki, M., and Matschinsky,
OBJECTIVE Previous studies have reported that β-cell mitochondria exist as discrete organelles exhibit heterogeneous bioenergetic capacity. To date, networking activity, and its role in mediating mitochondrial morphology function, remains unclear. In this article, we investigate fusion fission detail report alterations response to various combinations of nutrients. RESEARCH DESIGN AND METHODS Using matrix-targeted photoactivatable green fluorescent protein, were tagged tracked β-cells within...
The current classification system presents challenges to the diagnosis and treatment of patients with diabetes mellitus (DM), in part due its conflicting confounding definitions type 1 DM, 2 latent autoimmune adults (LADA). schema also lacks a foundation that readily incorporates advances our understanding disease treatment. For appropriate coherent therapy, we propose an alternate system. β-cell-centric DM is new approach obviates inherent unintended confusions model presupposes all...
The Banting Medal for Scientific Achievement Award is the American Diabetes Association's highest scientific award and honors an individual who has made significant, long-term contributions to understanding of diabetes, its treatment, and/or prevention. named after Nobel Prize winner Sir Frederick Banting, codiscovered insulin treatment diabetes. Dr. Barbara E. Corkey received at 71st Sessions, 24–28 June 2011, San Diego, California. She presented Lecture, “Hyperinsulinemia: Cause or...
Despite varied treatment, mitigation, and prevention efforts, the global prevalence severity of obesity continue to worsen. Here we propose a combined model obesity, unifying paradigm that links four general models: energy balance (EBM), based on calories as driver weight gain; carbohydrate-insulin (CIM), insulin storage; oxidation-reduction (REDOX), reactive oxygen species (ROS) altered metabolic signaling; obesogens (OBS), which proposes environmental chemicals interfere with hormonal...
Null point titration techniques have been developed for measurements of cytosolic free Mg2+ in isolated cells and matrix mitochondria using antipyrylazo III as a spectrophotometric indicator. A 0.37 +/- 0.02 mM was obtained with hepatocytes. This represented about 6% the total magnesium content (activity coefficient 5.8 X 10(-2). Nondiffusable Mg2+-binding sites cytosol were equal to 11.1 nmol/mg cell dry weight an apparent dissociation constant 0.71 accounted binding 32% magnesium. The null...
The hypothesis proposing that anaplerosis and cataplerosis play an important role in fuel signaling by providing mitochondrially derived coupling factors for stimulation of insulin secretion was tested. A rise citrate coincided with the initiation response to glucose INS-1 beta-cells. dose dependence glucose-stimulated release correlated closely those cellular contents citrate, malate, citrate-derived malonyl-CoA. glucose-induced elevations alpha-ketoglutarate, malonyl-CoA,...
To gain insight into the relationship between acyl coenzyme A (CoAf esters and glucose-induced insulin release, acyl-CoA profiles were determined in clonal pancreatic @-cells (HIT).A high sensitivity per- formance liquid chromatography method was used to measure malonyl, succinyl, &hydroxy 8-methylglutaryl acetyl-CoA free CoASH.Malonyl-CoA content increased more than %fold following exposure of HIT cells 10 m M glucose.The rise malonyl-CoA, which preceded secretion, evident 2 min after...
β3-Adrenergic receptors (β3-ARs) are expressed predominantly on white and brown adipocytes, acute treatment of mice with CL 316,243, a potent highly selective β3-AR agonist, produces 2-fold increase in energy expenditure, 50–100-fold insulin levels, 40–50% reduction food intake. Recently, we generated gene knockout lacking functional β3-ARs demonstrated that each these responses were mediated exclusively by β3-ARs. However, the tissue site responsible for producing actions is unknown. In...
Insulin secretion in the intact organism, and by perfused pancreas groups of isolated perifused islets, is pulsatile. We have proposed a metabolic model glucose-induced insulin which oscillations ATP/ADP ratio drive alterations electrical events that lead to release. A key prediction our metabolically driven Ca2+ will also occur. Using fluorescent probe, fura 2, digital image analysis, sensitive O2 electrodes, we investigated cytosolic free responses consumption rat islets had been...
Uncoupling protein (UCP) 2 is a widely expressed mitochondrial whose precise function still unclear but has been linked to mitochondria-derived reactive oxygen species production. Thus, the chronic absence of UCP2 potential promote persistent accumulation and an oxidative stress response. Here, we show that Ucp2−/− mice on three highly congenic (N >10) strain backgrounds (C57BL/6J, A/J, 129/SvImJ), including two independently generated sources Ucp2-null animals, all exhibit increased...