A5054176992- Parkinson's Disease Mechanisms and Treatments
- Alzheimer's disease research and treatments
- Parallel Computing and Optimization Techniques
- Distributed and Parallel Computing Systems
- Heat shock proteins research
- Plant tissue culture and regeneration
- Cellular transport and secretion
- Advanced Data Storage Technologies
- Neurological disorders and treatments
- Protein Structure and Dynamics
- Luminescence and Fluorescent Materials
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
- Transgenic Plants and Applications
- Embedded Systems Design Techniques
- Modular Robots and Swarm Intelligence
- Organic Electronics and Photovoltaics
- Legume Nitrogen Fixing Symbiosis
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Marine and coastal ecosystems
Shihezi University
2025
Ministry of Agriculture and Rural Affairs
2025
Xinjiang Production and Construction Corps
2025
Shanghai Institute of Organic Chemistry
2017-2024
Chinese Academy of Sciences
2018-2024
University of Chinese Academy of Sciences
2018-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2024
Anhui University of Science and Technology
2024
Tianjin University of Technology
2009-2023
Hebei GEO University
2011-2023
Abstract Subcellular membrane-less organelles consist of proteins with low complexity domains. Many them, such as hnRNPA1, can assemble into both a polydisperse liquid phase and an ordered solid amyloid fibril. The former mirrors biological granule assembly, while the latter is usually associated neurodegenerative disease. Here, we observe reversible formation hnRNPA1 that synchronizes liquid–liquid separation, regulates fluidity mobility liquid-like droplets, facilitates recruitment stress...
Significance Posttranslational modifications (PTMs) play an important role in modulating α-syn structure, function, and pathology. Phosphorylation commonly applies to pathological aggregation Lewy bodies is a hallmark of Parkinson’s disease (PD). Here, we obtain homogeneous fibrils formed by semisynthesized with site-specific phosphorylation at Y39. The synthetic fibril recapitulates the neuropathology degradation resistance endogenous pY39 PD. Cryo-EM structure determination reveals fold...
The genetic manipulation of Agrobacterium tumefaciens is used to facilitate studies bacterial gene functions or as a first step in introducing material into transformable plant cells through the use T-DNA binary vectors. Three methods are commonly used. Transformation with purified plasmid can be done either electroporation simple freeze/thaw transformation method. Alternatively, mobilizable placed using triparental mating Here we present three detailed protocols for strain construction...
BACKGROUNDBacterial infections of the genital tract are one most serious causes infertility in males. In some human patients with poor semen quality, leukocytospermia has been observed. Because leukocytes express bacterial-lipopolysaccharide (LPS) responsive Toll-like receptor (TLR) signaling cascade and secrete tumor necrosis factor-α, secreted cytokines comprise one, but probably not only, class factors that can impact sperm motility.
Amyloid aggregation of α-synuclein (α-syn) is closely associated with Parkinson's disease (PD) and other synucleinopathies. Several single amino-acid mutations (e.g. E46K) α-syn have been identified causative to the early onset familial PD. Here, we report cryo-EM structure an fibril formed by N-terminally acetylated E46K mutant (Ac-E46K). The represents a distinct fold α-syn, which demonstrates that mutation breaks electrostatic interactions in wild type (WT) thus triggers rearrangement...
Membraneless organelles contain a wide spectrum of molecular chaperones, indicating their important roles in modulating the metastable conformation and biological function membraneless organelles. Here we report that class I II Hsp40 (DNAJ) proteins possess high ability phase separation rendered by flexible G/F-rich region. Different localize different Specifically, human Hdj1 (DNAJB1), protein, condenses ubiquitin (Ub)-rich nuclear bodies, while Hdj2 (DNAJA1), nucleoli. Upon stress, both...
The spread of pathological α-synuclein (α-syn) is a crucial event in the progression Parkinson's disease (PD). Cell surface receptors such as lymphocyte activation gene 3 (LAG3) and amyloid precursor-like protein 1 (APLP1) can preferentially bind α-syn over monomeric state to initiate cell-to-cell transmission. However, molecular mechanism underlying this selective binding unknown. Here, we perform an array biophysical experiments reveal that LAG3 D1 APLP1 E1 domains commonly use alkaline...
Amyloid aggregation of pathological proteins is closely associated with a variety neurodegenerative diseases, and α-synuclein (α-syn) deposition Tau tangles are considered hallmarks Parkinson's disease Alzheimer's disease, respectively. Intriguingly, α-syn have been found to co-deposit in the brains individuals dementia parkinsonism, suggesting potential role cross-talk between these two pathologies. Here we show that monomeric variants Tau, Tau23 K19, synergistically promote amyloid...
Significance Amyloid deposition of α-syn is a hallmark PD. Heterozygous point mutations are associated with the early-onset and rapid progression fPD. In this study, we found fibril strain formed by familial mutant hE46K induced motor deficit enhanced aggregation in vivo. More importantly, showed through cryogenic electron microscopy vivo studies E46K can template wild-type monomer to form amyloid fibrils, which inherit both structure propagation potency template. Our work reveals structural...
Our ability to create systems with large amount of hardware parallelism is exceeding the average software developer's effectively program them. This a problem that plagues our industry. Since vast majority world's developers are not parallel programming experts, making it easy write, port, and debug applications sufficient core vector essential enabling use multi- many-core processor architectures. However, architectures ISAs also shifting diversifying quickly, difficult for single binary...
Vesicle associated membrane protein 2 (VAMP2/synaptobrevin2), a core SNARE residing on synaptic vesicles (SVs), forms helix bundles with syntaxin-1 and SNAP25 for the assembly. Prior to assembly, structure of VAMP2 is unclear. Here, by using in-cell NMR spectroscopy, we describe dynamic association motif in mammalian cells, structural change upon intracellular lipid environment. We analyze compositions SV mass-spectrometry-based lipidomic profiling, further reveal that distinctive...
Marselan wine is characterized by distinctive flavors of blackcurrant, cranberry, and spice, which are significantly influenced environmental factors such as region climate. In this study, we analyzed the dynamic changes in microbial community, physicochemical indices, flavor compounds during spontaneous fermentation Xinjiang using high-throughput sequencing (HTS), high-performance liquid chromatography (HPLC), headspace solid-phase microextraction gas chromatography–mass spectrometry...
α-Synuclein (α-Syn) forms pathological amyloid aggregates deposited in Lewy bodies and neurites the brain of Parkinson's disease (PD) patients. Heat shock proteins (Hsps) are major components cellular chaperone network, which responsible for preventing from aggregation. Different Hsps were reported to interact with α-syn. However, underlying mechanism interplay between α-syn different remains unclear. Here, by combing NMR spectroscopy, electron microscope other biochemical approaches, we...