A5057714592- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Cholesterol and Lipid Metabolism
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Muscle Physiology and Disorders
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Reproductive Biology and Fertility
- Peroxisome Proliferator-Activated Receptors
- Genetics, Aging, and Longevity in Model Organisms
- Cancer, Lipids, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Calpain Protease Function and Regulation
- Lipoproteins and Cardiovascular Health
- Telomeres, Telomerase, and Senescence
- Cell Adhesion Molecules Research
- Adipose Tissue and Metabolism
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Galectins and Cancer Biology
- Genetic Neurodegenerative Diseases
- Lipid metabolism and disorders
Jilin University
2015-2025
Biotechnology Research Institute
2022-2024
Baise University
2023
Union Hospital
2022
Biotechnology Research Institute
2021
Jimo District Qingdao Hospital of Traditional Chinese Medicine
2018-2021
Jilin Medical University
2014-2020
The First People's Hospital of Changzhou
2017
Pediatric Nephrology of Alabama
2013
University of Michigan
2010
Classical swine fever (CSF) caused by classical virus (CSFV) is one of the most detrimental diseases, and leads to significant economic losses in industry. Despite efforts many government authorities stamp out disease from national pig populations, remains widespread. Here, antiviral small hairpin RNAs (shRNAs) were selected then inserted at porcine Rosa26 (pRosa26) locus via a CRISPR/Cas9-mediated knock-in strategy. Finally, anti-CSFV transgenic (TG) pigs produced somatic nuclear transfer...
Insulin degrading enzyme (IDE) is believed to act as a junction point of Type 2 diabetes (T2D) and Alzheimer's disease (AD); however, the underlying mechanism was not completely clear yet. Transgenic APPSwe/PS1 mice were used AD model treated with streptozocin/streptozotocin (STZ) develop mixed presenting both T2D. Morris Water Maze (MWM) recognition task performed trace cognitive function. The detection fasting plasma glucose (FPG) insulin concentration, oral tolerance test (OGTT)...
Abstract Genetic mutations in the MYBPC3 gene encoding cardiac myosin binding protein C (cMyBP-C) are most common cause of hypertrophic cardiomyopathy (HCM). Myocardial fibrosis (MF) plays a critical role development HCM. However, mechanism for mutant -induced MF is not well defined. In this study, we developed R495Q pig model using cytosine base editing and observed an early-onset these pigs shortly after birth. Unexpectedly, found that “cardiac-specific” was actually expressed fibroblasts...
Genetically modified pigs have important roles in agriculture and biomedicine. However, genome-specific knock-in techniques are still their infancy optimal strategies not been extensively investigated. In this study, we performed electroporation to introduce a targeting donor vector (a non-linearized that did contain promoter or selectable marker) into Porcine Foetal Fibroblasts (PFFs) along with CRISPR/Cas9 vector. After optimization, the efficiency of EGFP site-specific could reach up...
Precise genome editing in livestock is of great value for the fundamental investigation disease modeling. However, genetically modified pigs carrying subtle point mutations were still seldom reported despite rapid development programmable endonucleases. Here, we attempt to investigate single-stranded oligonucleotides (ssODN) mediated knockin by introducing two orthologous pathogenic mutations, p.E693G Alzheimer's and p.G2019S Parkinson's disease, into porcine APP LRRK2 loci, respectively....
The fat-1 gene from Caenorhabditis elegans encodes a fatty acid desaturase which was widely studied due to its beneficial function of converting n-6 polyunsaturated acids (n-6PUFAs) n-3 (n-3PUFAs). To date, many transgenic animals have been generated study disease pathogenesis or improve meat quality. However, all them were using random integration method with variable transgene expression levels and the introduction selectable marker genes often raise biosafety concern. this end, we aimed...
Abstract Diabetes has become one of the major noninfectious diseases that seriously endanger public health. The formation islet amyloid polypeptide (IAPP) affects normal physiological functions body, such as glucose metabolism and lipid metabolism. mature human IAPP protein (hIAPP) a strong tendency to misfold is considered be causes changes in islets. Deposition hIAPP leading type 2 diabetes mellitus (T2DM). Miniature pigs are experimental animal models well suited for research on gene...
Valproic acid (VPA), a histone deacetylase inbibitor, has been shown to generate inducible pluripotent stem (iPS) cells from mouse and human fibroblasts with significant higher efficiency. Because successful cloning by somatic cell nuclear transfer (SCNT) undergoes full reprogramming process in which the epigenetic state of differentiated donor is converted into an embryonic totipotent state, we speculated that VPA would be useful promoting Therefore, present study, examined whether can...
<b><i>Background:</i></b> Apoptosis is recognized as an important mechanism in contrast-induced nephropathy (CIN). As tetramethylpyrazine (TMP) has been recently found to be renoprotective and anti-apoptotic multiple kidney injuries, we hypothesized that TMP would prevent CIN. <b><i>Methods:</i></b> An experimental model of CIN was established rats. Serum creatinine, blood urea nitrogen, plasma cystatin C, urinary N-acetyl-β-glucosaminidase,...
Hemophilia B is an X-linked recessive bleeding disorder caused by abnormalities in the coagulation factor IX gene. Without prophylactic treatment, patients experience frequent spontaneous episodes. Well-characterized animal models are valuable for determining pathobiology of disease and testing novel therapeutic innovations. Here, we generated a porcine model hemophilia using combination CRISPR/Cas9 somatic cell nuclear transfer. Moreover, tested possibility therapy gene insertion. Frequent...
Abstract: To investigate the therapeutic effects of cyclodextrin on development atherosclerosis in rabbits, we evaluated (2-hydroxypropyl)-β-cyclodextrin (HPβCD) therapy organ coefficient, lipid profiles, inflammatory cytokines, and atherosclerotic plaques rabbits fed a high-fat diet. Our results demonstrated that HPβCD reduced plasma triglyceride levels cytokine but increased high-density lipoprotein cholesterol levels. produced significant decrease lesion area macrophage collagen content...
Contents MicroRNAs (miRNAs) are a class of small, non‐coding RNAs approximately 22 nucleotides in length that regulate gene expression by binding to the 3′‐untranslated regions target mRNAs. It is now clear miRNAs involved many biological processes, including proliferation, differentiation and regulation during early embryonic development. The miRBase 16.0 (2010) shows there 175, 673, 408 1048 annotated for Caenorhabditis elegans , Mus musculus Rattus norvegicus Homo sapiens respectively....
Resveratrol is a naturally occurring polyphenolic compound with known cardioprotective, anti-inflammatory, and antioxidant properties. Lipoprotein-associated phospholipase A2 (Lp-PLA2 ) associated the risk of cardiovascular disease. Here, we investigated effects resveratrol on Lp-PLA2 expression in vitro vivo explored underlying mechanisms.Human monocytic cells (THP-1) were induced to differentiate into macrophages for an experimental model. suppressed reduced inflammation;...
Apolipoprotein CIII (apo CIII), a small glycoprotein that binds to the surfaces of certain lipoproteins, is associated with inflammatory and atherogenic responses in vascular cells. Lipoprotein-associated phospholipase A2 (Lp-PLA2) has been proposed as an biomarker potential therapeutic target for cardiovascular disease (CVD). Here, we report apo increases Lp-PLA2 mRNA protein levels dose- time- dependent manner human monocytic THP-1 cells, increase can be abolished by MAPK NFκB pathway...
The consumption of n-3 polyunsaturated fatty acids (PUFAs) is important to human health, especially in cases cardiovascular disease. Although beneficial effects PUFAs have been observed a number studies, the mechanisms involved these yet be discovered.We generated hfat-1 transgenic pigs with traditional somatic cell nuclear transfer (SCNT) technology. acid composition ear tissue were detected gas chromatography. cholesterol, triglycerides (TAG) and inflammation mediators circulation...
This study was conducted to establish pig embryonic stem (ES)-like cell lines from nuclear transfer blastocysts. A green fluorescent protein (GFP)-expressing line used as the source of donor cells injected into enucleated oocytes. Blastocysts were collected at D5 (the fifth day), D7 seventh day) and D9 ninth day). Differential staining assay viability development blastocysts 3 days. The number inner mass (ICM) increased 1.83 ± 0.8 (D5) 5.37 1.2 (D7) 7.56 1.5 (D9). expression profiles (ES)...