A5100391186- Chronic Kidney Disease and Diabetes
- Salivary Gland Disorders and Functions
- Gout, Hyperuricemia, Uric Acid
- Renal Diseases and Glomerulopathies
- Renal and Vascular Pathologies
- Cancer, Hypoxia, and Metabolism
- Retinal Imaging and Analysis
- Pharmacological Effects of Natural Compounds
- Hemoglobin structure and function
- Acute Kidney Injury Research
- Obstructive Sleep Apnea Research
- Parathyroid Disorders and Treatments
- Biomedical Research and Pathophysiology
- Nitric Oxide and Endothelin Effects
- Diabetes and associated disorders
- Cell Adhesion Molecules Research
- Peroxisome Proliferator-Activated Receptors
- Infrared Thermography in Medicine
- Exercise and Physiological Responses
- Dermatology and Skin Diseases
- Acne and Rosacea Treatments and Effects
- Retinal Diseases and Treatments
- Medical Imaging Techniques and Applications
- MRI in cancer diagnosis
- Thermoregulation and physiological responses
State Key Laboratory of Kidney Diseases
2018-2025
Chinese People's Liberation Army
2018-2025
National Clinical Research Center for Digestive Diseases
2020-2025
Guiyang Medical University
2022-2025
Mayo Clinic in Arizona
2024
Chinese PLA General Hospital
2020-2024
Affiliated Hospital of Hebei University
2024
Shanxi Medical University
2022-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Hunan University of Technology
2024
We studied catalysis by tetrahydrobiopterin (H4B)-free neuronal nitric-oxide synthase (nNOS) to understand how heme and H4B participate in nitric oxide (NO) synthesis. H4B-free nNOS catalyzed Arg oxidation toN ω-hydroxy-l-Arg (NOHA) citrulline both NADPH- H2O2-driven reactions. Citrulline formation was time- enzyme concentration-dependent but uncoupled relative NADPH oxidation, generated nitrite nitrate without forming NO. Similar results were observed when NOHA served as substrate....
Context Diabetic retinopathy (DR) and diabetic nephropathy (DN), are major microvascular complications of diabetes. DR is an important predictor DN, but the relationship between severity pathological glomerulopathy remains unclear. Objective To investigate histological changes clinical indicators (DN) in patients with type 2 diabetes mellitus (T2DM) Methods Patients T2DM (n=272) who underwent a renal biopsy were eligible. Severity was classified as non-diabetic retinopathy, non-proliferative...
This work develops and validates an electrochemical approach for uric acid (UA) determinations in both endogenous (cell lysate) physiological (serum) samples. is based on the electrocatalytic reduction of enzymatically generated H2O2 at biosensor uricase−thionine−single-walled carbon nanotube/glassy (UOx−Th−SWNTs/GC) with use Th−SWNTs nanostructure as a mediator enzyme immobilization matrix. The biosensor, which was fabricated by immobilizing UOx surface Th−SWNTs, exhibited rapid response...
Abstract Rosacea is a chronic inflammatory skin disorder with high incidence rate. Although genetic predisposition to rosacea suggested by existing evidence, the basis remains largely unknown. Here we present integrated results of whole genome sequencing (WGS) in 3 large families and exome (WES) 49 additional validation families. We identify single rare deleterious variants LRRC4, SH3PXD2A SLC26A8 families, respectively. The relevance SH3PXD2A, LRR family genes underscored presence...
To understand how heme and (6R)-5,6,7,8-tetrahydro-l-biopterin (H(4)B) participate in nitric-oxide synthesis, we followed ferrous-dioxy (Fe(II)O(2)) formation disappearance, H(4)B radical formation, Arg hydroxylation during a single catalytic turnover by the inducible synthase oxygenase domain (iNOSoxy). In all cases, prereduced (ferrous) enzyme was rapidly mixed with an O(2)-containing buffer to start reaction. A intermediate formed quickly (53 s(-1)) then decayed concurrent buildup of...
Abstract High-fat diet (HFD) is widely used in animal models of many diseases, it helps to understand the pathogenic mechanism related diseases. Several dietary fats were commonly HFD, such as corn oil, peanut soybean sunflower and lard. However, was reported that different fat could have completely effects on physiological indicators gut microbiome, sources high-fat research not been comprehensively compared. In this research, we conduct comparative experiments various test their during...
Background The disease pathology for diabetes mellitus patients with chronic kidney (CKD) may be diabetic nephropathy (DN), non-diabetic renal (NDRD), or DN combined NDRD. Considering that the prognosis and treatment of NDRD differ, their differential diagnosis is significance. Renal pathological biopsy gold standard diagnosing However, it invasive cannot implemented in many due to contraindications. This article constructed a new noninvasive evaluation model differentiating Methods We...
Background Dysbiosis of the gut microbiota is closely related to chronic systemic inflammation and autoimmunity, playing an essential role in pathogenesis primary Sjögren’s syndrome (pSS). Abnormalities proportions blood T lymphocyte subtype, that Th17/Treg, were detected pSS patients. We aimed determine associations between Th17/Treg pSS. Method 98 patients 105 healthy controls (NC) enrolled Dec 1, 2018, Aug 31, 2019. The baseline information clinical parameters on collected. 16S rRNA...
Facial microbiota dysbiosis is an important factor in causing acne vulgaris. The present study aimed to analyze the effect of 2% Supramolecular Salicylic Acid (SSA) on acne-associated facial bacteria. In current study, 30 vulgaris patients (treated with SSA for eight weeks) and ten volunteers no were selected. Samples from (before after treatment) (not treated) analyzed via high throughput sequencing, Deblur algorithm, R microbiome package. After treatment SSA, total lesion count global...
Pulmonary fibrosis (PF) emerges as a significant pulmonary sequelae in the convalescent phase of coronavirus disease 2019 (COVID-19), with current strategies neither specifically preventive nor therapeutic. Geum japonicum var. chinense (GJC) is used traditional Chinese medicine to effectively treat various respiratory conditions. However, protective effects GJC against PF remains unclear. In present study, anti-PF effect aqueous extract was studied using mouse model induced by bleomycin...
<b><i>Background:</i></b> Apoptosis is recognized as an important mechanism in contrast-induced nephropathy (CIN). As tetramethylpyrazine (TMP) has been recently found to be renoprotective and anti-apoptotic multiple kidney injuries, we hypothesized that TMP would prevent CIN. <b><i>Methods:</i></b> An experimental model of CIN was established rats. Serum creatinine, blood urea nitrogen, plasma cystatin C, urinary N-acetyl-β-glucosaminidase,...
The aim of the present study was to analyze clinical characteristics primary Sjogren's syndrome (pSS) with neuromyelitis optica spectrum disorder (NMOSD). We retrospectively reviewed medical records 616 patients who were admitted Peking Union Medical College Hospital from 1985 2013. Of these patients, 43 developed NMOSD. median duration symptoms 60 months and 72% experienced neurological complications onset in pSS NMOSD group. Twenty-one out had (NMO), 22 exhibited a limited form NMO. Serum...
Abstract Background Estimated glomerular filtration rate (eGFR) has become incorporated into multiple clinical management situations. Historically, equations included a Black race coefficient, which lacked biological plausibility and created potential to exacerbate health disparities. A new equation in 2021 changed the weighting of age, sex, creatinine by modeling against diverse cohort removing coefficient. Content variety outcomes including kidney disease risk stratification, medication...
Diabetic nephropathy (DN) is a major cause of end-stage kidney disease, and therefore early diagnosis intervention may help reverse renal damage. One hundred sixty-eight patients with T2DM 56 healthy volunteers (control group) were enrolled at Shandong University Qilu Hospital between April 2010 October 2012. All subjects underwent blood sampling for sera homocysteine (Hcy) cystatin C (Cys C) assays urine microalbumin test. The divided into three groups according to the excretion rate...
A ferrous heme-NO complex builds up in rat neuronal NO synthase during catalysis and lowers its activity. Mutation of a tryptophan located directly below the heme (Trp409) to Phe or Tyr causes hyperactive synthesis less buildup steady state (Adak, S., Crooks, C., Wang, Q., Crane, B. R., Tainer, J. A., Getzoff, E. D., Stuehr, D. (1999) Biol. Chem. 274, 26907–26911). To understand mechanism, we used conventional stopped flow spectroscopy compare kinetics formation, enzyme activity prior after...
2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is the most abundant mutagen/carcinogen produced in high temperature treated protein food. Quercetin has been shown to inhibit PhIP formation by trapping phenylacetaldehyde form two human beneficial adducts 6-C-(E-phenylethenyl)quercetin (6-CEPQ) and 8-C-(E-phenylethenyl)quercetin (8-CEPQ). Here we studied of as well phenylacetaldehyde-trapping ability quercetin microwave heating conventional systems. LC-MS was applied for comparison...
Objectives Chronic kidney disease (CKD) is a common chronic condition with high incidence and insidious onset. Glomerular injury (GI) tubular (TI) represent early manifestations of CKD could indicate the risk its development. In this study, we aimed to classify GI TI using three machine learning algorithms promote their diagnosis slow progression CKD. Methods Demographic information, physical examination, blood, morning urine samples were first collected from 13,550 subjects in 10 counties...
Polygonum capitatum as an ethnic medicine has been used to treat urinary tract infections, pyelonephritis and calculi. In our previous study, P. was found have anti-hyperuricemia effects. Nevertheless, the active constituents of for treating hyperuricemia were still unclear. this ultra-high-performance liquid chromatography coupled quadrupole/orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) comprehensively detect chemical ingredients its absorbed in plasma rats first time....
Lack of effective drugs for acute kidney injury (AKI) grades 1-2 is a crucial challenge in clinic. Our previously single-center clinical studies indicated Chuan Huang Fang (CHF) might have nephroprotection AKI on chronic disease (CKD) (A C) patients by preventing oxidant damage and inhibiting inflammation. Reduced glutathione (RG) has recently been shown to increase the effectiveness high-flux hemodialysis among with severe AKI. In this multicenter randomized controlled study, we designed...