A5100637517- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Agriculture and Biological Studies
- Hepatitis B Virus Studies
- Genetic diversity and population structure
- Medicinal plant effects and applications
- Animal Genetics and Reproduction
- Hepatitis C virus research
- Renal and related cancers
- Sperm and Testicular Function
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Gynecological conditions and treatments
- Corneal surgery and disorders
- Ovarian function and disorders
- Corneal Surgery and Treatments
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Virology and Viral Diseases
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- Ocular Surface and Contact Lens
- Malaria Research and Control
- Monoclonal and Polyclonal Antibodies Research
- Bioenergy crop production and management
Utah State University
2007-2025
Affiliated Hospital of Guizhou Medical University
2019-2024
Guiyang Medical University
2019-2024
South China University of Technology
2022-2024
University of Southern California
2024
Tianjin Centers for Disease Control and Prevention
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2011-2021
Tianjin Medical University
2021
Tianjin Infectious Diseases Hospital
2021
State Key Laboratory of Medical Molecular Biology
2021
Significance Transfer of sperm-producing stem cells isolated from a donor male into testes recipient has important applications for preserving germplasm and as conduit widespread dissemination desirable genetics in livestock production. A key aspect is surrogate males that lack endogenous germline but are otherwise physiologically normal. Here we demonstrate mice, pigs, goats rendered genetically sterile by CRISPR-Cas9 editing the NANOS2 gene support donor-derived spermatogenesis following...
Vaccination against hepatitis B virus is an effective and routine practice that can prevent infection. However, 5–10% of healthy adults fail to produce protective levels antibody the vaccination. It has been reported host genetic variants might affect immune response Here, we a genome-wide association study in Chinese Han population consisting 108 primary high-responders 77 booster non-responders vaccination using Illumina HumanOmniExpress Beadchip. We identified 21 SNPs at 6p21.32 were...
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare genetic disorder for which no cure exists. The disease characterized by premature aging and inevitable death in adolescence due to cardiovascular complications. Most HGPS patients carry a heterozygous de novo LMNA c.1824C > T mutation, provokes the expression of dominant-negative mutant protein called progerin. Therapies proven effective HGPS-like mouse models have yielded only modest benefit clinical trials. To overcome gap...
Abstract Interspecies somatic cell nuclear transfer (iSCNT) has the capacity to rescue endangered species, ethically produce human embryonic stem cells, and allow for more specific analysis of pre‐implantation embryos. However, several obstacles negatively affect iSCNT efficiency. Over 100 studies have been published between 1999 spring 2023. This review details possible benefits iSCNT, describes hypothesized issues that prevented development some embryos and/or live offspring from...
ABSTRACT Currently, incubators with a time‐lapse system are widely used for in vitro embryo production several species, however, their effect on alpaca development compared to conventional remains unknown. The aim of this study was compare early alpacas using incubator versus incubator. Ovaries were obtained from slaughterhouse and 1048 cumulus‐oocyte complexes (COCs) collected matured 26 h either ( n = 542) or 542). All the COCs fertilised 24 fresh spermatozoa recovered epididymis...
Pluripotent stem cells (PSCs) have been successfully developed in many species. However, the establishment of bovine-induced pluripotent (biPSCs) has challenging. Here we report generation biPSCs from bovine mesenchymal (bMSCs) by overexpression lysine-specific demethylase 4A (KDM4A) and other reprogramming factors OCT4, SOX2, KLF4, cMYC, LIN28, NANOG (KdOSKMLN). These exhibited silenced transgene expression at passage 10, had prolonged self-renewal capacity for over 70 passages. The flat,...
In Brief Purpose: To investigate the concentrations and pharmacokinetics of ketorolac in rabbits by three different routes administrations: a single intracameral, intravitreal, suprachoroidal injection. Methods: Fifty-four New Zealand white received (250 μg/0.05 mL) one eye intracameral injection (group A, n = 18), intravitreal B, C, 18). Drug vitreous, retina–choroid (RC), plasma were determined methods high-performance liquid chromatography at 0.5, 1, 2, 4, 8, 24 hours after The opposite...
Transgenic porcine cancer models bring novel possibilities for research. Their physical similarities with humans enable the use of surgical procedures and treatment approaches used patients, which facilitates clinical translation. Here, we aimed to develop an inducible oncopig model intestinal cancer. (TG) minipigs were generated using somatic cell nuclear transfer by handmade cloning. The pigs encode two TG cassettes: (a) Flp recombinase-inducible oncogene cassette containing KRAS-G12D,...
Hu sheep is a valuable breed in China, and semen cryopreservation of important for sustainable development the agri-food industry. This study aimed to evaluate effect thawing rate antioxidants (procyanidins [PC] mitoquinone [MitoQ]) on quality antioxidant enzyme activity post-thaw sperm sheep. Our results showed that highest was obtained from group thawed at 70°C 5 seconds. Furthermore, addition 150 nM MitoQ extender could enhance motility, integrity membrane acrosome, mitochondrial...
Human papillomavirus type 16 plays a critical role in the neoplastic transformation of cervical cancers. Molecular variants HPV16 existing different ethnic groups have shown substantial phenotypic differences pathogenicity, immunogenicity and tumorigenicity. In this study, we sequenced entire genome 76 isolates originated from Anyang, central China. Phylogenetic analysis these sequences identified two major Anyang area, namely European prototype (E(p)) Asian (E(As)). These show high degree...
Abstract The immune response to hepatitis B vaccination varies among individuals. It has been reported that polymorphisms in cytokine and receptor genes are associated with these individual differences. aim of the current study was investigate association between Th1/Th2 a Chinese Han population. A total 10 single nucleotide distributed 6 (TNFRSF1A, IL12A, IL12B, IFNG, IL4, IL10) were genotyped 214 high‐responders [hepatitis surface antibody (anti‐HBs) ≥1,000 mIU/ml] 107 low‐responders...
Summary Light exposure is a common stress factor during in vitro handling of oocytes and embryos that originates from both microscope ambient light. In the current study, effect two types light (daylight laboratory light) on porcine parthenogenetically activated (PA) was tested experiments: (1) medium subsequently used for embryo development; (2) before development. The results Experiment 1 showed culture to decreased percentage blastocysts good morphology, only after 24 h exposure. 2...
Abstract: To investigate the therapeutic effects of cyclodextrin on development atherosclerosis in rabbits, we evaluated (2-hydroxypropyl)-β-cyclodextrin (HPβCD) therapy organ coefficient, lipid profiles, inflammatory cytokines, and atherosclerotic plaques rabbits fed a high-fat diet. Our results demonstrated that HPβCD reduced plasma triglyceride levels cytokine but increased high-density lipoprotein cholesterol levels. produced significant decrease lesion area macrophage collagen content...
Aims and background.Cell membranes were shown to be sensitive affected by static magnetic fields (SMF). Methods. Cells treated with four anticancer drugs followed treatment a combination of SMF. Individual cells examined using atomic force microscopy (AFM). The taxol (alkaloid), doxorubicin (anthracycline), cisplatin (platinum compound) cyclophosphamide (alkylating agent). Results. Holes observed in exposed SMF but not control groups. number, size shape the holes dependent on drug type,...
To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus. A total 20 cases healthy donor corneas were group A, and patients (20 eyes) suffered from fungal keratitis (FK) composed B. Real-time qPCR immunohistochemistry applied to detect dectin-1 epithelium both groups. HCE cultured with aspergillus fumigatus (AF) antigens vitro. The mRNA was measured real-time at stimulation 0, 4, 8 24h separately. Dectin-1...
The present study is to measure the expression of programmed death (PD)-1 / ligand-1 (PD-L1) negative costimulatory molecules, soluble format sPD-1 in patients with immune thrombocytopenia (ITP), and investigate their correlation secretion cytokines. A total 35 ITP were included study. Twenty healthy subjects who received physical examination at our hospital as control group. Peripheral blood was collected from all subjects. Flow cytometry performed determine percentages PD-1+CD4+T cells...
Composite proteins with designable nutritional and functional properties are attracting great attention in the food industry. In this study, novel protein composites were fabricated by co-dissolving zein soy isolate (SPI) at pH 12. Different ratios of vs. (S/Z) showed different characteristics. The supernatant composite S/Z = 1 was indicated a better solubility, balanced amino acid ratio, digestibility (98.74%); 0.01 exhibited higher free radical scavenging ability (>90%,5 mg/mL) lower...
To evaluate the effect of Collagen cross-linking on prevention melting in rabbit corneas after alkali burn.