A5035703104- Sperm and Testicular Function
- Genetic and phenotypic traits in livestock
- Animal Behavior and Welfare Studies
- Regulation of Appetite and Obesity
- Birth, Development, and Health
- Reproductive Biology and Fertility
- Adipose Tissue and Metabolism
- Animal Nutrition and Physiology
- Adipokines, Inflammation, and Metabolic Diseases
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Biochemical Analysis and Sensing Techniques
- Effects and risks of endocrine disrupting chemicals
- Child Nutrition and Water Access
- Chromosomal and Genetic Variations
- Effects of Environmental Stressors on Livestock
- Meat and Animal Product Quality
- Bee Products Chemical Analysis
- Fatty Acid Research and Health
- Virus-based gene therapy research
- Data Analysis with R
- Xenotransplantation and immune response
- Selenium in Biological Systems
- Animal Virus Infections Studies
- Genetic Mapping and Diversity in Plants and Animals
Universidad de Extremadura
2023
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
1996-2022
Institute of Agrifood Research and Technology
2019-2022
Centro Regional de Selección y Reproducción Animal
1991-2021
Hospital Universitario La Paz
2010
Justus-Liebig-Universität Gießen
1991
The objective of the present study was to determine suitability a swine breed with leptin resistance and predisposition obesity (the Iberian pig) as model for studies on metabolic syndrome type 2 diabetes. Thus, six sows had ad libitum access food enriched saturated fat (SFAD group; consumption estimated be 4.5 kg/animal/day) whilst four females acted controls were fed kg/animal/day commercial maintenance diet. After three months differential feeding, SFAD animals developed central obesity,...
Hutchinson-Gilford progeria syndrome (HGPS) is an extremely rare genetic disorder for which no cure exists. The disease characterized by premature aging and inevitable death in adolescence due to cardiovascular complications. Most HGPS patients carry a heterozygous de novo LMNA c.1824C > T mutation, provokes the expression of dominant-negative mutant protein called progerin. Therapies proven effective HGPS-like mouse models have yielded only modest benefit clinical trials. To overcome gap...
The effects of undernutrition during pregnancy on prenatal and postnatal development the offspring were evaluated in sows with obesity/leptin resistance. Females fed, from day 35 onwards, a diet fulfilling either 100% (group control, n=10) or 50% nutritional requirements underfed, n=10). In control group, maternal body weight increased (P<0.05) while it decreased remained steady underfed group. At days 75 100 gestation, plasma triglycerides lower but urea levels higher restricted than...
Three-dimensional bioprinting is a technology in constant development, mainly due to its extraordinary potential revolutionize regenerative medicine. It allows fabrication through the additive deposition of biochemical products, biological materials, and living cells for generation structures bioengineering. There are various techniques biomaterials or bioinks that suitable bioprinting. Their rheological properties directly related quality these processes. In this study, alginate-based...
Hydroxytyrosol is a polyphenol with antioxidant, metabolism-regulatory, anti-inflammatory and immuno-modulatory properties. The present study aimed to determine whether supplementing the maternal diet hydroxytyrosol during pregnancy can improve pre- early post-natal developmental patterns metabolic traits of offspring. Experiment was performed in Iberian sows fed restricted order increase risk IUGR. Ten were treated daily 1.5 mg per kg feed between Day 35 (30% total gestational period) until...
Recently, the capacity of mesenchymal stem/stromal cells (MSCs) to migrate into damaged tissues has been reported. For MSCs be a promising tool for tissue engineering and cell gene therapy, it is essential know their migration ability according origin. However, little known about molecular mechanisms regulating porcine MSC chemotaxis. The aim this study was examine migratory properties in an inflammatory environment lines from different origins: subcutaneous adipose (SCA-MSCs), abdominal...
Maternal malnutrition during pregnancy, both deficiency and excess, induces changes in the intrauterine environment metabolic status of offspring, playing a key role growth, fitness/obesity appearance disorders postnatal life. There is increasing evidence that these effects may not be only limited to first generation descendants, offspring directly exposed challenges, but subsequent generations. This study evaluated, swine model obesity/leptin resistance, existence extent transgenerational...
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...
Perfluorooctane sulfonate (PFOS) and perfluorohexane (PFHxS) are toxic bioaccumulative, included in the Stockholm Convention's list as persistent organic pollutants. Due to their toxicity, worldwide distribution, lack of information spermatozoa physiology during pre-fertilization processes, present study seeks analyze effects possible alterations caused by presence these compounds boar sperm vitro capacitation. The capacitation was performed supplemented TALP-Hepes media mean lethal...
The objective of this study was to determine, in a female swine model leptin resistance (Iberian pig), the effect an obesogenic diet, with high saturated fat content, during juvenile period, on appearance early obesity and its possible effects metabolic syndrome-related parameters reproductive features (puberty attainment). Thus, from 130 245 days-old, seven Iberian gilts had ad libitum access food enriched whilst six females acted as controls commercial maintenance diet. Results showed that...
The present study aimed to determine the effects of breed and sex on growth patterns metabolic features advanced-pregnancy foetuses exposed same environmental conditions. Thus, at Day 62 pregnancy, swine from an obese with leptin resistance (Iberian breed) were compared lean crossbred (25% Large White ×25% Landrace ×50% Pietrain). There differential developmental in resistance, mainly a higher relative weight brain resembling "brain-sparing effect". Prioritization may be protective for...
Twenty‐five gilts without measurable serum antibody titres to porcine reproductive and respiratory syndrome virus (PRRsv) were identified 16 inoculated with PRRSV at seven, 14 or 21 days of gestation killed 20 22 later determine the effect on their embryos. The remaining nine not exposed PRRSV, but same stages gestation. gults observed for clinical signs infection embryos tested PRRv homologous antibodies. was demonstrated by re‐isolation its detection reverse transcriptase polymerase chain...
The present study aimed to determine whether developmental patterns, adiposity level and fatty-acid composition of fetuses exposed maternal malnutrition are driven by their sex or genotype, both, as these may modulate the adaptive response intrauterine environment independently genotype. We used a single genotype (purebred Iberian (IB) sows), which was inseminated with heterospermic semen (obtained mixing from Large White (LW) boars), obtain four different subsets (male female, purebred...
Contents The Iberian pig is an autochthonous breed of the Mediterranean area, reared extensively in central and southern areas Spain Portugal that known worldwide for production ham. characteristics ham are related to its abundance intramuscular fat, owing high capacity accumulate fat under skin between muscular fibres. This ability store excess enables survival during periods scarcity it has been found other antique animal breeds even humans, being named as thrifty genotype . reproductive...
The incidence of obesity and metabolic diseases is dramatically high in rapidly developing countries. Causes have been related to intrinsic ethnic features with development a thrifty genotype for adapting food scarcity, prenatal programming by undernutrition, postnatal exposure obesogenic lifestyle. Observational studies humans experimental animal models evidence that the adaptive responses offspring may be modulated their sex. In contemporary context world globalization, new question...
Sex-related differences in lipid availability and fatty acid composition during swine foetal development were investigated. Plasma cholesterol triglyceride concentrations the mother strongly related to adequacy or inadequacy of concomitant activation protective growth some organs (brain, heart, liver spleen). Cholesterol was similar male female offspring, but foetuses showed evidence higher placental transfer essential acids synthesis non-essential muscle liver. These sex-related affected...
Abstract The aim of this study was to assess whether vulvar morphometric changes occurring in female pigs during proestrus and oestrus could be objective, accurate predictive indicators the onset thus performed artificial inseminations at most appropriate time. For that purpose, pictures vulvas from 60 hyperprolific females (30 gilts 30 sows) were taken once a day. Vulva measurements (area, perimeter, length width) on these using image processing ImageJ software. Gilts sows showed...
Contents The main aim of this study was to document the prevalence chromosomal aberrations found date on pig population in Spain, a country which production sector has critical role, being fourth world and second one within European Union. total number animals studied 849, founded frequency carrier pigs with alterations 3.8%. When only structural were considered, males 3.3%. This percentage is far from 0.5% boars that been estimated France, where there systematic cytogenetic screening future...