A5091113023- Reproductive Biology and Fertility
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Genetic and phenotypic traits in livestock
- Prenatal Screening and Diagnostics
- Reproductive Physiology in Livestock
- Assisted Reproductive Technology and Twin Pregnancy
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Animal Behavior and Welfare Studies
- Sperm and Testicular Function
- Sexual Differentiation and Disorders
- Calcium signaling and nucleotide metabolism
- Cytomegalovirus and herpesvirus research
- Hernia repair and management
- Cancer-related gene regulation
- Testicular diseases and treatments
- Genetic Mapping and Diversity in Plants and Animals
- Virus-based gene therapy research
- Reproductive Health and Technologies
Universidad de Murcia
2018-2025
University of Évora
2022
Instituto Murciano de Investigación Biosanitaria
2019-2022
Assisted reproductive technologies (ART) are routinely used in livestock to generate animals of high genetic value. Despite representing an outstanding accomplishment, recent studies suggest differences health, fertility, and gestational length vitro-produced compared vivo-derived animals. Currently, there no data available on the long-term effects ART growth development. This observational study aimed understand relationship between growth-influencing hormones a herd cattle derived from...
Abstract Preimplantation embryos experience profound resetting of epigenetic information inherited from the gametes. Genome-wide analysis at single-base resolution has shown similarities but also species differences between human and mouse preimplantation in DNA methylation patterns reprogramming. Here, we have extended such to two key livestock species, pig cow. We generated genome-wide whole-transcriptome datasets gametes blastocysts both species. In oocytes a distinctive bimodal landscape...
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...
The increasing use of in vitro embryo production (IVP) followed by transfer (ET), alongside with cryopreservation embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One hypotheses for these alterations is current culture conditions IVP. In an attempt to better mimic physiological milieu, embryos were produced female reproductive fluids (RF) as supplements medium, and another group supplemented bovine serum albumin (BSA) control....
Large/abnormal offspring syndrome (LOS/AOS) is a congenital overgrowth reported in ruminants produced by assisted reproduction (ART-LOS) which exhibit global disruption of the epigenome and transcriptome. LOS/AOS shares phenotypes epigenotypes with human condition Beckwith-Wiedemann syndrome. We have that LOS occurs spontaneously (SLOS); however, to date, no study has been conducted determine if SLOS same methylome epimutations as ART-LOS. In this study, we performed whole-genome bisulphite...
Abstract Background In vitro embryo production (IVP) and transfer (ET) are two very common assisted reproductive technologies (ART) in human cattle. However, pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to low efficiency IVP plus difficulty performing ET long contorted uterus sow. addition, potential impact these ART on health offspring unknown. We investigated here if a modified system, with natural fluids (RF) as supplements...
Abstract The addition of reproductive fluids (RF) to the culture media has shown benefits in different embryonic traits but its long-term effects on offspring phenotype are still unknown. We aimed describe such pigs. Blood samples and growth parameters were collected from piglets derived vitro-produced embryos (IVP) with or without RF added versus those artificially inseminated (AI), day 0 month 6 life. An oral glucose tolerance test was performed 45 show here first comparative data animals...
Limb girdle muscular dystrophy type R1 (LGMDR1) is an autosomal recessive myopathy described in humans resulting from a deficiency of calpain-3 protein (CAPN3). This disease lacks effective treatment and appropriate model, so the generation KO pigs by CRISPR-Cas9 offers way to better understand ethology develop novel therapies. Microinjection main method for gene editing porcine embryo, but electroporation, which allows handling more embryos faster easier, has also recently been reported....
Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing higher collection of female gametes. However, consequences this for the gamete or offspring have been shown several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to responses. In work, we use bovine model each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing...
Assisted reproductive technologies play a major role in the cattle industry. An increase use of vitro -derived embryos is currently being seen around globe. But efficiency and quality are substandard when compared to vivo production. Different protocols have been designed overcome this issue, one those fluids as supplementation embryo culture media. In study, calves produced with added their production protocol were followed for first year life pairwise control, by artificial insemination...
Assisted reproductive technologies (ART), besides solving several problems, it has also been used as a tool to improve the animal productivity that is required for feeding human population. One of these techniques, embryo transfer (ET), presented limitations in porcine species, which could constrain its use industry. To clarify potential this technique, we aimed compare impact using ET or artificial insemination (AI) on phenotype offspring during first days age, terms growth and blood...
Abstract Large/abnormal offspring syndrome (LOS/AOS) is a congenital overgrowth reported in ruminants produced by assisted reproduction (ART-LOS) which exhibit global disruption of the epigenome and transcriptome. LOS/AOS shares phenotypes epigenotypes with human condition Beckwith-Wiedemann syndrome. We have that LOS occurs spontaneously (SLOS), however, to date, no study has been conducted determine if SLOS same methylome epimutations as ART-LOS. In this study, we performed whole genome...
Assisted reproductive technologies (ART) are being extensively used to produce cattle offspring. However, as shown by Siqueira et al. (2017 J. Dairy Sci. 100, 5899-5908; https://doi.org/10.3168/jds.2016-12539), phenotypical and performance differences between cows derived from distinct ART can be found at different stages of development. Thus, in an attempt mimic the natural environment, fluids (oviductal (SOF) uterine fluids) were added supplementation embryo culture media. Our hypothesis...
Abstract Background The increased use of assisted reproductive technologies led to a higher risk specific alterations in the offspring, as already described human and other mammalian species. Nonetheless, it is yet be totally understood how which techniques are inducing these changes. animal models may represent an advantage find out answers questions due minimization confounding factors. pig not only great model, but also interest livestock industry develop further that enhance meat...
Abstract Background : In vitro embryo production (IVP) and transfer (ET) are two very common assisted reproductive technologies (ART) in human cattle. However, pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to low efficiency IVP plus difficulty performing ET long contorted uterus sow. addition, potential impact these ART on health offspring unknown. We investigated here if a modified system, with natural fluids (RF) as supplements...
Abstract Background : In vitro embryo production (IVP) and transfer (ET) are two very common assisted reproductive technologies (ART) in human cattle. However, pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to low efficiency IVP plus difficulty performing ET long contorted uterus sow. addition, potential impact these ART on health offspring unknown. We investigated here if an improved system, with natural fluids (RF) as supplements...