A5068425350- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- CRISPR and Genetic Engineering
- Ovarian function and disorders
- Virus-based gene therapy research
- Xenotransplantation and immune response
- Genetic and phenotypic traits in livestock
- RNA Interference and Gene Delivery
- Reproductive Health and Technologies
- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- Effects of Environmental Stressors on Livestock
- Animal health and immunology
- Animal Behavior and Welfare Studies
- Advanced biosensing and bioanalysis techniques
- Comparative International Legal Studies
- Medical Imaging Techniques and Applications
- Viral Infections and Vectors
- Mechanical Engineering and Vibrations Research
- Assisted Reproductive Technology and Twin Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Pregnancy and preeclampsia studies
- Mechanical Failure Analysis and Simulation
Universidad de Murcia
2013-2025
Instituto Murciano de Investigación Biosanitaria
2018-2023
Empresarios Agrupados
2019
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2004-2009
Applus+ IDIADA (Spain)
1997-2009
Instituto de Biotecnología de León
2002
University of Pittsburgh
1997
This study evaluated the effects of serum (egpS) and follicular fluid (egpFF) from estrus guinea pigs as in vitro maturation (IVM) supplements for natural cycle pig oocytes. Two experiments were conducted: first assessed three IVM media, a commercial medium (CMOM) two homemade media (HMOM-P HMOM-S), oocyte using 615 bovine oocytes, while second incorporation 5%, 10%, 20% egpFF or egpS into 1744 Initially, we optimized base oocytes to determine most suitable culture conditions. The results...
Sperm-mediated gene transfer (SMGT) is a method for the production of transgenic animals based on intrinsic ability sperm cells to bind and internalize exogenous DNA molecules them into oocyte at fertilization. Recombinase-A (RecA) protein-coated has been used previously in pronuclear injection systems increasing integration goat pig genomes. However, there are no data regarding transgene expression after ICSI. Here, we set out investigate whether porcine embryos improved by...
Contents A porcine in vitro fertilization (IVF) system and seminal quality parameters of frozen–thawed boar semen were used to assess the effectiveness two different thawing rates frozen semen, address question whether differences between fertility ejaculates could be predicted a limited field trial. In first experiment, procedures analysed (37°C, 30 s; 50°C, 12 s) no sperm found. However, when procedure was s IVF results showed higher number per penetrated oocyte near 10 points rate...
The objectives of this study were to evaluate: (1) the nuclear maturation, (2) intracellular glutathione (GSH) content, (3) normality fertilization and (4) full development after transplantation embryos derived from porcine oocytes pre-cultured with 50 micromol/l roscovitine (an inhibitor p34cdc2/cyclin B kinase) for 22 h. After treatment roscovitine, configuration (Hoechst staining) was comparable those examined just collection: majority arrested at germinal vesicle (GV) 1 stage (63.2%)....
This study was designed to determine the effect of different sperm preparation treatments before IVF on acrosome reaction, oocyte penetration time, early embryo development and timing female male pronucleus formation. Pooled sperm-rich fractions were (i) washed in PBS, (ii) left unwashed, or (iii) layered a Percoll gradient. In Expt 1, proportion acrosome-reacted spermatozoa, determined by staining with fluorescein isothyocyanate-labelled peanut agglutinin lectin propidium iodide, highest...
The evolution of some fetal growth indices and arterial blood flow parameters in the umbilical cord embryos fetuses primiparous pregnant goats Murciano-Granadina breed were analyzed by ultrasonography. Weekly ultrasonographic sessions took place from 18- to 125-days post-breeding. Fetal measures carried out ultrasound B-mode. This mode was used take a series measurements embryo/fetus throughout pregnancy: crown-rump length (CRL, 24-days post-mating -dpm- 61 dpm), trunk diameter (TD, 24–34...
Abstract Background In vitro embryo production (IVP) and transfer (ET) are two very common assisted reproductive technologies (ART) in human cattle. However, pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to low efficiency IVP plus difficulty performing ET long contorted uterus sow. addition, potential impact these ART on health offspring unknown. We investigated here if a modified system, with natural fluids (RF) as supplements...
Artificial insemination (AI) with frozen semen in goats still presents serious difficulties, especially certain goat breeds, spite of technological progress. The aim this work is the vitro study seminal extenders adapted from those used on other species to evaluate response sperm several homeostatic conditions order achieve optimal post-thaw quality. Three different based activity principles were used: (1) extender according methodology proposed for pigs, (2) skimmed-milk-based France, and...
ABSTRACT Fourteen single and two double point mutants in the highly conserved region (positions 56 to 159) of G gene viral hemorrhagic septicaemia virus (VHSV), a salmonid rhabdovirus, were selected obtained plasmids by site-directed mutagenesis. Fish cell monolayers transfected with mutant then assayed for protein (pG) expression, conformation-dependent monoclonal antibody (MAb) reactivity, cell-cell fusion. Some mutations located phospholipid-binding p2 peptide 82 110; P86A, A96E, G98A,...
Controlled ovarian stimulation is a necessary step in some assisted reproductive procedures allowing higher collection of female gametes. However, consequences this for the gamete or offspring have been shown several mammals. Most studies used comparisons between oocytes from different donors, which may contribute to responses. In work, we use bovine model each animal serves as its own control. DNA methylation profiles were obtained by single-cell whole-genome bisulfite sequencing...