A5018484236- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Ovarian function and disorders
- Reproductive Physiology in Livestock
- Bee Products Chemical Analysis
- Reproductive biology and impacts on aquatic species
- Reproductive System and Pregnancy
- Reproductive Health and Technologies
- Assisted Reproductive Technology and Twin Pregnancy
- Nitric Oxide and Endothelin Effects
- Hormonal and reproductive studies
- Animal Behavior and Welfare Studies
- Agriculture and Biological Studies
- CRISPR and Genetic Engineering
- Effects of Environmental Stressors on Livestock
- Male Reproductive Health Studies
- Proteins in Food Systems
- Selenium in Biological Systems
- Primate Behavior and Ecology
- Xenotransplantation and immune response
- Extracellular vesicles in disease
- Birth, Development, and Health
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Universidad de Murcia
2014-2024
Instituto Murciano de Investigación Biosanitaria
2014-2023
Hospital Universitario Virgen del Rocío
2020
Institute for the International Education of Students
2016
Universidad Autónoma del Estado de Morelos
2014
Czech Academy of Sciences, Institute of Physiology
2009
University of Veterinary Science
2007
Wonkwang University
2004
University of Eastern Finland
2004
Universidade de São Paulo
2004
Polyspermy is an important anomaly of fertilization in placental mammals, causing premature death the embryo. It especially frequent under vitro conditions, complicating successful generation viable embryos. A block to polyspermy develops as a result changes after sperm entry (i.e., cortical granule exocytosis). However, additional factors may play role regulating by acting on gametes before sperm–oocyte interaction. Most studies have used rodents models, but ungulates differ mechanisms...
Abstract Differences between the in vitro and vivo environment which fertilization occurs seem to play a key role low efficiency of porcine (IVF). This work proposes an IVF system based on oviductal periovulatory environment. The combined use medium at pH found oviduct stage (pH e 8.0), mixture components (cumulus-oocyte complex secretions, follicular fluid fluid, OFCM) device that interposes physical barrier gametes (an inverted screw cap Falcon tube, S) was compared with classical 7.4,...
In this study, we evaluated the effects of glutathione (L-gamma-glutamyl-L-cysteinylglycine; GSH) supplementation freezing extender on semen parameters during cooling (2 hours at 5 degrees C) and phases cryopreservation process to compensate for decrease in GSH content observed sperm freezing. To fully address these questions, incorporated a new set functional tests. These included tests mitochondrial function, inducibility acrosome reaction, vitro penetration (IVP) oocytes, changes...
One of the proposed mechanisms polyspermy block is an increased resistance zona pellucida (ZP) to proteolytic digestion (ZP hardening) as a consequence cortical granule exocytosis that occurs soon after fertilization. However, evidence available zonae pellucidae freshly ovulated pig and cow oocytes harden considerably before It was thought such pre-fertilization ZP hardening could be involved in control polyspermy, its lack matured vitro one reasons for extremely high incidence fertilization...
ABSTRACT: In this study, we evaluated the effects of glutathione (L‐g‐glutamyl‐L‐cysteinylglycine; GSH) supplementation thawing extender on semen parameters to compensate for decrease in GSH content observed during sperm freezing. To fully address these questions, used a set functional tests. These included tests motility and motion parameters, changes sulfhydryl group membrane proteins, capacitation status, measures intra‐cellular reactive oxygen species generation, chromatin condensation,...
In mammals, fertilization and early pre-implantation development occur in the oviduct. Previous results obtained our laboratory have identified specific molecules oviduct that affect porcine sperm-egg interactions. The aim of present study was to determine whether contact between oocytes oviductal fluid also embryo development, quality, gene expression. vitro matured were exposed bovine (bOF) for 30 min prior fertilization. Cleavage blastocyst rates significantly higher from bOF-treated than...
Sperm-mediated gene transfer (SMGT) is a method for the production of transgenic animals based on intrinsic ability sperm cells to bind and internalize exogenous DNA molecules them into oocyte at fertilization. Recombinase-A (RecA) protein-coated has been used previously in pronuclear injection systems increasing integration goat pig genomes. However, there are no data regarding transgene expression after ICSI. Here, we set out investigate whether porcine embryos improved by...
Contents A porcine in vitro fertilization (IVF) system and seminal quality parameters of frozen–thawed boar semen were used to assess the effectiveness two different thawing rates frozen semen, address question whether differences between fertility ejaculates could be predicted a limited field trial. In first experiment, procedures analysed (37°C, 30 s; 50°C, 12 s) no sperm found. However, when procedure was s IVF results showed higher number per penetrated oocyte near 10 points rate...
Spermatozoa transport through the oviduct is a controlled process that regulates sperm capacitation. A crucial event involved in capacitation protein tyrosine phosphorylation (TP). This study was undertaken to determine whether similarities exist TP distribution between spermatozoa bound or unbound oviductal epithelial cells (OEC) three different conditions: i) vitro, coincubated with OEC cultures; ii) ex vivo, deposited porcine explants from slaughtered animals; iii) which sows were...
The in vivo concentration of bicarbonate (HCO3 -), one the essential sperm capacitating effectors, varies greatly different environments go through from cauda epididymis to fertilisation site. On contrary, porcine vitro capacitation and media usually contains a standard 25 mmol/L, main problems presented is unacceptable high incidence polyspermy. This work hypothesised that by modifying HCO3 - medium, output could be increased. Once exposed intracellular pH (pHi) spermatozoa increased...
Physiological events at the time of fertilization pig oocytes may differ in vitro depending on (IVF) medium. This hypothesis was tested by maturation for 44 h NCSU-37 medium and thereafter with frozen-thawed ejaculated spermatozoa. Three different IVF media (TCM-199, Tyrode's albumin lactate pyruvate (TALP) Tris-buffered (TBM)) were used. For acrosome reaction test, spermatozoa incubated 0-150 min three media, proportion live acrosome-reacted acrosome-intact cells determined fluorescein...
The objectives of this study were to evaluate: (1) the nuclear maturation, (2) intracellular glutathione (GSH) content, (3) normality fertilization and (4) full development after transplantation embryos derived from porcine oocytes pre-cultured with 50 micromol/l roscovitine (an inhibitor p34cdc2/cyclin B kinase) for 22 h. After treatment roscovitine, configuration (Hoechst staining) was comparable those examined just collection: majority arrested at germinal vesicle (GV) 1 stage (63.2%)....
Studies of knockout (KO) mice with defects in the endolysosomal two-pore channels (TPCs) have shown TPCs to be involved pathophysiological processes, including heart and muscle function, metabolism, immunity, cancer, viral infection. With objective studying TPC2's roles for first time a large, more humanlike animal model, TPC2 KO pigs were produced using CRISPR-Cas9. A major problem CRISPR-Cas9 edit embryos is mosaicism; thus, we studied effect microinjection timing on mosaicism. Mosaicism...