A5000878372- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Prenatal Screening and Diagnostics
- Birth, Development, and Health
- Reproductive Physiology in Livestock
- Ovarian function and disorders
- Telomeres, Telomerase, and Senescence
- Bioenergy crop production and management
- Tissue Engineering and Regenerative Medicine
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive System and Pregnancy
- Genetic Syndromes and Imprinting
- Demographic Trends and Gender Preferences
- Genetic and phenotypic traits in livestock
- Rabbits: Nutrition, Reproduction, Health
- Genetics, Aging, and Longevity in Model Organisms
- Reproductive Health and Technologies
- Spaceflight effects on biology
- Molecular Biology Techniques and Applications
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2010-2024
Consejo Superior de Investigaciones Científicas
2022-2024
Centro Regional de Selección y Reproducción Animal
2008-2021
Institute for Research and Technology in Food and Agriculture
2021
Institute for Animal Reproduction
2019-2020
Instituto Nacional de Tecnologia
2016
University of Maryland, College Park
2012-2015
United States Department of Agriculture
2012-2014
University of Missouri
2011-2012
Beltsville Agricultural Research Center
2012
Genetic and environmental factors produce different levels of DNA damage in spermatozoa. Usually, DNA-fragmented spermatozoa (DFS) are used with intracytoplasmic sperm injection (ICSI) treatments human reproduction, use DFS is still a matter concern. The purpose the present study was to investigate long-term consequences on development behavior mice generated by ICSI DFS. Using CD1 B6D2F1 mouse strains, oocytes were injected fresh or frozen-thawed without cryoprotector. This treatment...
Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate differences at the mRNA level, we compared gene-expression patterns between blastocysts by DNA microarray comparison of nine groups 60 bovine in vitro-produced each sex. Almost one-third transcripts detected showed sexual dimorphism (2,921 transcripts; false-discovery rate, P < 0.05), suggesting that absence hormonal influences,...
Elevated concentrations of serum non-esterified fatty acids (NEFA), associated with maternal disorders such as obesity and type II diabetes, alter the ovarian follicular micro-environment have been subfertility arising from reduced oocyte developmental competence. We asked whether elevated NEFA during maturation affect development physiology zygotes formed oocytes, using cow a model. The were grown to blastocysts, which evaluated for their quality in terms cell number, apoptosis, expression...
Epigenetic differences between male and female bovine blastocysts provide a plausible link physiological gene transcription observed embryos. The aim of this study was to examine sex-related epigenetic in produced vitro. Oocytes were matured vitro inseminated with frozen-thawed sex-sorted (X or Y) unsorted (control) bull sperm. Zygotes cultured blastocyst stage analyzed for embryo sexing, mtDNA content, telomere lengths, methylation analysis, quantification mRNA transcripts DNA...
The aim of this study was to evaluate the effect extracellular vesicles (EV) from oviductal fluid (OF), either ampulla or isthmus, on development and quality in vitro -cultured bovine embryos. Zygotes were cultured synthetic oviduct (SOF + 3 mg/mL BSA) without calf serum (C− group), presence × 10 5 EV/mL ampullary isthmic OF at 1 4 g (10 K) (100 K), compared with SOF 5% FCS (C+ group). OF-EV size concentration assessed by electron microscopy nanotracking analysis system. Embryo recorded Days...
The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable sperm-egg membrane fusion: the sperm IZUMO1 and SPACA6, egg protein JUNO. Here we demonstrate that another protein, TMEM95, necessary interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this were morphologically normal exhibited motility, could penetrate zona pellucida bind...
Abstract Genetic mosaicism is the presence of more than two alleles on an individual and it commonly observed following CRISPR microinjection zygotes. This phenomenon appears when DNA replication precedes CRISPR-mediated genome edition undesirable because reduces greatly odds for direct KO generation by randomly generated indels. In this study, we have developed alternative protocols to reduce rates in bovine. a preliminary study EdU incorporation that has already occurred at conventional...
Abstract The ratio of male/female embryos may be modified by environmental factors such as maternal diet in vivo and the composition embryo culture media vitro. We have used amino acid profiling, a noninvasive marker developmental potential to compare effect sex on metabolism bovine blastocysts conceived Blastocysts were incubated individually for 24 hr close‐to‐physiological mixture acids depletion or appearance 18 measured using HPLC. then sexed PCR. Amino vitro‐produced expanded was...
Using bovine embryos generated in vitro from IVF with X-sorted, Y-sorted and unsorted spermatozoa, we compared the kinetics of male female embryo development gene expression between blastocysts. Bovine vitro-matured oocytes (n = 8858) were fertilised spermatozoa each three different bulls (X-sorted, or depending on experiment). The cleavage rate was assessed 24, 27, 30, 33, 36, 40, 44 48 h post insemination (h.p.i.) blastocyst recorded Days 6–9. relative mRNA abundance nine genes (GSTM3,...
To evaluate the effect of bovine oviductal fluid (OF) supplementation during in vitro culture embryos on their development and quality, vitro-produced zygotes were cultured synthetic (SOF; negative control; C-) supplemented with OF or 5% fetal calf serum (positive C+). Embryo was recorded Days 7-9 after insemination blastocyst quality assessed through cryotolerance, differential cell counting inner mass trophectoderm, gene expression. added to medium at concentrations ranging from 0.625%...
Does maternal obesity affect estrous cyclicity, embryo development and blastocyst gene expression in mice? Maternal alters cyclicity causes the down-regulation of two key metabolite receptors (Slc2a1 Ldlr) blastocysts recovered from diet-induced obese females, but is not affected. reduces fertility because effects periconception period, its negative influence on oocyte quality or development. This was a randomized study based mouse model for obesity. Twenty-one outbred NIH Swiss mice were...
Sex chromosome transcripts can lead to a broad transcriptional sexual dimorphism in the absence of concomitant or previous exposure sex hormones, especially when X-chromosome inactivation (XCI) is not complete. XCI timing has been suggested differ greatly among species, and bovine, most X-linked are upregulated female blastocysts. To determine XCI, we analyzed day 14 bovine embryos dimorphic transcription seven genes known be blastocysts ( X24112 , brain-expressed 2 BEX2 ),...
Offspring telomere length (TL) has been correlated with paternal TL, but the mechanism for this parent of origin-specific inheritance remains unclear. The objective study to determine role spermatozoa TL in embryonic lengthening by using two mouse models showing dimorphism their TL: Mus musculus vs spretus and old young musculus. displayed a shorter than Hybrid offspring exhibited lower compared starting at two-cell stage, before onset telomerase expression. To analyze telomeres early...
Physical exercise has positive effects on cognition, but very little is known about the inheritance of these to sedentary offspring and mechanisms involved. Here, we use a patrilineal design in mice test transmission from same father (before or after training) different fathers compare sedentary- runner-father progenies. Behavioral, stereological, whole-genome sequence analyses reveal that paternal cognition improvement inherited by offspring, along with increased adult neurogenesis, greater...
Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, ploidy in vitro produced bovine embryos. Subsequently, using real-time PCR, the expression genes expected to underlie observed at blastocyst stage. matured oocytes were either fertilized or activated with ionomycin +6-DMAP cultured simple medium....
It has been reported that the mammalian female could have a preconceptual influence on sex of her offspring, and it hypothesized this go some way toward accounting for lower fertility following insemination with sex-sorted sperm. To test whether in vitro matured oocytes are able to select X- or Y-bearing spermatozoa fertilization (IVF), we fertilized 1788 X-sorted semen, Y-sorted mix unsorted semen from same bull, cultured until Day 9. Fertility was assessed by recording cleavage rate at 48...
A high-glucose concentration in the reproductive tract during early development may result aberrant embryo or fetal development, with effects that could have a greater impact on one sex than other. Here, we determine if impacts and pregnancy outcomes sex-specific manner mouse. Zygotes were cultured potassium simple optimized medium, which typically contains 0.2 mM D-glucose, without additional glucose supplementation to of 28 mM. Zygote cleavage blastocyst rate did not differ between...
Abstract In the recently published article “Amino acid metabolism of bovine blastocysts: A biomarker sex and viability” by R. Sturmey et al. (Mol Reprod Dev, volume 77 (3): 285–296, 2010 [DOI: 10.1002/mrd.21145]), Acknowledgment grant support was inadvertently omitted. The omitted text is herein printed to address this error: RGS wishes acknowledge financial from a Wellcome Trust VIP Fellowship, administered Biology Department University York. HJL Leverhulme Trust.