A5017889473- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive biology and impacts on aquatic species
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Animal Genetics and Reproduction
- Birth, Development, and Health
- RNA Research and Splicing
- Reproductive Health and Technologies
- Renal and related cancers
- Reproductive System and Pregnancy
- Cannabis and Cannabinoid Research
- Aquaculture Nutrition and Growth
- Assisted Reproductive Technology and Twin Pregnancy
- Genomics and Chromatin Dynamics
- Marine Invertebrate Physiology and Ecology
- Micro and Nano Robotics
- Orbital Angular Momentum in Optics
- Molecular Biology Techniques and Applications
- Spaceflight effects on biology
- Tardigrade Biology and Ecology
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Bioactive Compounds in Plants
Institute for Research and Technology in Food and Agriculture
2011-2021
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2011-2021
Institute for Animal Reproduction
2020
Centro Regional de Selección y Reproducción Animal
2011-2019
Hospital Universitario Puerta de Hierro Majadahonda
2016-2018
Weizmann Institute of Science
2011-2015
Universidad de León
2008-2011
Cryopreservation is of interest not only for fish farming but also the conservation and genetic improvement resources. This technique has been well established in some freshwater species mainly, salmonid, sturgeons carps, however, last decade research was focused marine species. The benefits sperm cryopreservation include: (i) synchronization gamete availability both sexes, (ii) economy; (iii) simplification broodstock management, (iv) transport gametes from different farms, (v) germplasm...
Does a novel antioxidant formulation designed to restore redox balance within the male reproductive tract, reduce sperm DNA damage and increase pregnancy rates in mouse models of oxidative stress? Oral administration significantly reduced glutathione peroxidase 5 (GPX5), knockout mice restored near-normal levels subjected scrotal heat stress. Animal human studies have documented adverse effect on fertilization rates, embryo quality, miscarriage transfer de novo mutations offspring. Semen...
Abstract A unique characteristic of mammalian sperm thermotaxis is extreme temperature sensitivity, manifested by the capacity spermatozoa to respond changes <0.0006 °C as they swim their body-length distance. The identity sensing system that confers this exceptional sensitivity on not known. Here we show temperature-sensing involves opsins, known be G-protein-coupled receptors act photosensors in vision. We demonstrate molecular, immunological and functional approaches opsins are present...
The fusion of gamete membranes during fertilization is an essential process for sexual reproduction. Despite its importance, only three proteins are known to be indispensable sperm-egg membrane fusion: the sperm IZUMO1 and SPACA6, egg protein JUNO. Here we demonstrate that another protein, TMEM95, necessary interaction. TMEM95 ablation in mice caused complete male-specific infertility. Sperm lacking this were morphologically normal exhibited motility, could penetrate zona pellucida bind...
Mammalian spermatozoa undergo a strong selection process along the female tract to guarantee fertilization by good quality cells, but risks of with DNA-damaged have been reported. In contrast, most external fertilizers such as fish seem weaker procedures. This fact, together their high prolificacy and embryo development, indicates that could be useful for study effects sperm DNA damage on development. We cryopreserved from rainbow trout using egg yolk low-density lipoprotein additives...
What is the behavioral mechanism underlying response of human spermatozoa to a temperature gradient in thermotaxis?Human swim up by modulating their speed and frequencies hyperactivation events turns.Capacitated are capable thermotactically responding with an outcome swimming gradient. This occurs even when very shallow.Human sperm samples were exposed fast change. A quantitative analysis motility parameters, flagellar wave propagation, directional changes before, during, after change was...
The ejaculate is a heterogeneous pool of spermatozoa containing only small physiologically adequate subpopulation for fertilization. As there no method to isolate this subpopulation, its specific characteristics are unknown. This one the main reasons why we lack effective tools identify male infertility and low efficiency assisted reproductive technologies. aim study was improve ICSI outcome by sperm selection through thermotaxis. Here show that mouse human can be selected in vitro...
Endocannabinoids have been recognized as mediators of practically all reproductive events in mammals. However, little is known about the role this system oocyte maturation. In a mouse model, we observed that activation cannabinoid receptor 1 (CB1) during vitro maturation modulated phosphorylation status Akt and ERK1/2 enhanced subsequent embryo production. absence CB1, vivo was impaired development delayed. Cannabinoid 2 (CB2) unable to rescue these effects. Finally, confirmed abnormal...
Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role biotechnologies scarce poultry. The aims of present study were to amino acids profile and total proteins seminal 12 Spanish chicken breeds investigate on cryoresistance rooster sperm. To cryoresistance, diluted pooled semen samples cryopreserved presence absence plasma. Glutamic acid was most abundant free plasma, followed by alanine, serine, valine, glycine. There an...
Oviductal microenvironment generally provides better conditions for early embryo development than the conventional in vitro system. In an attempt to simulate oviduct or main potentially influencing factors, effect was studied of a bovine oviductal fluid (bOF) treatment applied prior IVF on (i) parameters, (ii) cleavage rate, (iii) blastocyst yield and (iv) quality. Embryo quality assessed by morphological relative transcript abundance several developmental genes blastocysts. Furthermore,...
Highlights•ZRSR1mu produces severe defects in erythrocytes, muscle stretch, and spermatogenesis•Spermatogenesis of ZRSR1mu are due to splicing alteration U12-type intron•ZRSR1mu causes retention most the introns examined•ZRSR1 is implicated also removal U2 positioned adjacent a U12 intronSummaryThe U2AF35-like ZRSR1 has been recognition 3′ splice site during spliceosome assembly, but knockout mice do not show abnormal phenotypes. To analyze function its precise role RNA splicing, we...
Abstract Background Some adult stem cells persist in tissue; however, we do not know how to stimulate adults heal injuries. Liver growth factor (LGF) is a biliprotein with hepatic mitogen activity. Its concentration increases markedly the presence of any type liver injury, and it shows vivo therapeutic biological activity at extrahepatic sites. Methods We have analyzed effect LGF on replenishment germinal testes mice injected busulfan, common cancer drug that also specifically affects germ...
Zygotic repair of the paternal genome is a key event after fertilization. Spermatozoa accumulate DNA strand breaks during spermatogenesis and can suffer additional damage by different factors, including cryopreservation. Fertilization with DNA-damaged spermatozoa (DDS) considered to promote implantation failures abortions, but also long-term effects on progeny that could be related defective repair. Base excision (BER) pathway most active in zygotic repair, healthy oocytes contain enzymes...