A5063205640- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Renal and related cancers
- Chronic Lymphocytic Leukemia Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- CAR-T cell therapy research
- Reproductive Physiology in Livestock
- Demographic Trends and Gender Preferences
- RNA regulation and disease
- Chronic Myeloid Leukemia Treatments
- Birth, Development, and Health
- Reproductive Health and Technologies
- Ovarian function and disorders
- Cancer-related Molecular Pathways
- Genetic and phenotypic traits in livestock
- Acute Lymphoblastic Leukemia research
- RNA Interference and Gene Delivery
- Immune Cell Function and Interaction
Universidad Autónoma de Madrid
2014-2023
Centro Nacional de Biotecnología
2010-2023
Centro de Biología Molecular Severo Ochoa
2014-2023
Consejo Superior de Investigaciones Científicas
2008-2022
Instituto Ramón y Cajal de Investigación Sanitaria
2019-2022
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
1997-2013
Ferrer Grupo (Spain)
2013
Universitat de Miguel Hernández d'Elx
2013
Ikerbasque
2013
École Nationale Vétérinaire de Toulouse
2013
The long-term developmental and behavioral consequences of mammalian embryo culture are unknown. By altering the medium with addition FCS, we wanted to determine whether mouse embryos cultured under suboptimal conditions develop aberrant mRNA expression imprinting genes at blastocyst stage fetal development, growth, behavior adult mice affected. One-cell obtained from superovulated female B6CBAF 1 were for 4 days in K + -modified simplex optimized presence either 10% FCS or g/liter BSA....
Using reverse transcriptase-amplified fragment length polymorphism (RT-AFLP) analysis of differential mRNA expression and semiquantitative transcriptase-polymerase chain reaction, we compared in bovine blastocysts from 4 sources, known to differ quality terms their ability withstand cryopreservation: 1) vitro culture synthetic oviduct fluid vitro-matured (IVM)/in fertilized (IVF) zygotes; 2) TCM-199 supplemented with granulosa cells (coculture) IVM/IVF 3) vivo the ewe or 4) superovulation,...
The objective of this study was to examine the time during postfertilization period that gene expression patterns in vitro-cultured bovine embryos diverge from those their vivo-cultured counterparts. Presumptive zygotes were produced by vitro maturation and fertilization immature oocytes collected ovaries slaughtered animals. Approximately 20 h post insemination (hpi), denuded randomly divided into two groups for culture either vitro, synthetic oviduct fluid medium, or vivo, ewe oviduct....
Abstract Evidence exists to suggest detrimental effects of heat stress on male fertility. This study was designed assess the scrotal mature and developing sperm in a mouse model. After receiving shock treatment (42°C for 30 min), spermatozoa were recovered from epididymis hours (6) or Days (7, 14, 21, 28, 60) later, determine variables: number spermatozoa, viability, motility progressive motility, DNA integrity as established by TUNEL method, embryo implantation rate, sex ratio fetuses...
Abstract Recent data have demonstrated that fast‐cleaving embryos produced in vitro are more likely to develop blastocyst stage, and the postfertilization culture system used impacts considerably on mRNA expression quality of blastocysts produced. The present study is first investigate relationship between developmental speed vivo or temporal transcription pattern. Genes related important preimplantation events monitored during 4 days embryo development with fast slow development. set genes...
Neuropathic pain is a clinical manifestation of nerve injury difficult to treat even with potent analgesic compounds. Here, we used different lines genetically modified mice clarify the role played by CB(2) cannabinoid receptors in regulation central immune responses leading development neuropathic pain. knock-out and wild-type littermates were exposed sciatic injury, both genotypes developed similar hyperalgesia allodynia ipsilateral paw. Most strikingly, knock-outs also contralateral...
A new variant of Creutzfeldt Jacob Disease (vCJD) was identified in humans and linked to the consumption Bovine Spongiform Encephalopathy (BSE)-infected meat products. Recycling ruminant tissue bone meal (MBM) has been proposed as origin BSE epidemic. During this epidemic, sheep goats have exposed BSE-contaminated MBM. It is well known that can be experimentally infected with two field BSE-like cases reported goats. In work we evaluated human susceptibility small ruminants-passaged prions by...
Abstract Acute inflammation can either resolve through immunosuppression or persist, leading to chronic inflammation. These transitions are driven by distinct molecular and metabolic reprogramming of immune cells. The anti-diabetic drug Metformin inhibits acute mechanisms still not fully understood. Here, we report that the anti-inflammatory reactive-oxygen-species-inhibiting effects depend on expression plasticity factor ZEB1 in macrophages. Using mice lacking Zeb1 their myeloid cells human...
The effect of glucose in the medium used during vitro culture on sex ratio bovine blastocysts derived from in-vitro-matured andin-vitro-fertilized oocytes was evaluated. Oocytes were matured and inseminated with mixed sperm three bulls cultured modified synthetic oviducal fluid 10% fetal calf serum, or without supplementation. overall rate cleaved embryos that developed to expanded blastocyst (27.0%) significantly greater (P<0.05) than percentage observed when (17.5%). Analysis variance...
Nerve injuries often lead to neuropathic pain syndrome. The mechanisms contributing this syndrome involve local inflammatory responses, activation of glia cells, and changes in the plasticity neuronal nociceptive pathways. Cannabinoid CB 2 receptors contribute containment by modulating glial response nerve injury. Thus, spreads mice lacking beyond site To further investigate leading enhanced manifestation pain, we have established expression profiles spinal cord tissues from wild-type...
The ability of prions to infect some species and not others is determined by the transmission barrier. This unexplained phenomenon has led belief that certain were susceptible transmissible spongiform encephalopathies (TSEs) therefore represented negligible risk human health if consumed. Using protein misfolding cyclic amplification (PMCA) technique, we able overcome barrier in rabbits, which have been classified as TSE resistant for four decades. Rabbit brain homogenate, either unseeded or...
The potentially unbalanced expression at preimplantation developmental stages of X-linked genes might be responsible the faster development male than female embryos in vitro. Two located on X chromosome, glucose-6-phosphate dehydrogenase (G6PD) and hypoxanthine phosphoribosyl transferase (HPRT), are involved controlling amount oxygen radicals, hence they have influence embryo development. We quantified mRNA these two genes, using vitro fertilized-in cultured bovine embryos. In vitro-produced...
We have previously demonstrated that there is a clear relationship between the time interval insemination and first cleavage in vitro development to blastocyst stage of bovine embryos. In addition we shown this developmental ability can be linked stability mRNA for several gene transcripts measured 2-cell embryos cleaving at different times. The aim study was examine embryo competence, assessed terms cleavage, expression insulin-like growth factor-I (IGF-I) ligand receptor, hypoxanthine...
Abstract In the cyclic cow, final maturation of ovulatory follicle is initiated by preovulatory luteinizing hormone (LH) surge. During subsequent 24 hr period, oocyte nucleus undergoes meiotic progression to metaphase II and several changes in cytoplasmic organization take place. We have previously shown that oocytes recovered at time LH peak matured vitro are less competent reach blastocyst stage than their counterparts 20 later following vivo maturation, despite both groups undergoing IVF...
During the preimplantation period, in vitro cultured males have a higher metabolic rate, different gene expression, and grow faster than females. It has been suggested that under some stress conditions male embryos are more vulnerable females; however, biological fragility of is little understood. Since many forms result overproduction cellular reactive oxygen species (ROS), we addressed hypothesis connection between female advantage during early developmental stages heat involves ROS...
This study was designed to assess whether nucleic acid stains such as propidium iodide and Hoechst 33258 the cytosolic stain eosin identified equivalent proportions of non-viable cells. Sub-samples boar spermatozoa stored for up 72 h, frozen bull in straws thawed before staining, were exposed either or alone combination. Additional sub-samples stained with eosin-nigrosin subsequently Giemsa. The proportion cells by that observed when it used combination other fluorescent probe. Similar...
The aim of this study was to explore how some reproductive methodologies may affect the sex ratio. We first confirmed association between maturation stage bovine oocytes at time in vitro fertilisation (IVF) and ratio vitro-derived embryos. Secondly, we studied whether insemination, prior or after ovulation, could alter sheep. In experiment, were matured for 16 h; then either fertilised immediately extrusion polar body IVF delayed 8 h. proportion cleaving embryos their development 8-cell...