A5101677711- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- RNA regulation and disease
- Trace Elements in Health
- Animal Genetics and Reproduction
- Violence, Education, and Gender Studies
- Nursing care and research
- Agricultural and Food Production Studies
- Alcoholism and Thiamine Deficiency
- Psychological Treatments and Disorders
- Organizational Management and Innovation
Universidad Libre de Colombia
2022
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2004-2014
Centro Regional de Selección y Reproducción Animal
2003-2005
Centro de Investigación en Sanidad Animal
2003-2005
The specific characteristics of Transmissible Spongiform Encephalopathy (TSE) strains may be altered during passage across a species barrier. In this study we investigated the biochemical and biological Bovine (BSE) after transmission in both natural host (cattle, sheep, pigs mice) transgenic mice overexpressing corresponding cellular prion protein (PrPC) comparison with other non-BSE related prions from same species. After these passages, most features BSE agent remained unchanged....
The bovine-porcine species barrier to bovine spongiform encephalopathy (BSE) infection was explored by generating transgenic mouse lines expressing the porcine prion protein (PrP) gene. All of (poTg) mice showed clinical signs BSE after intracerebral inoculation with a high-titer inoculum. protease-resistant PrP (PrP res ) detected in 14% (3 22) BSE-infected poTg immunohistochemical or immunoblot analysis. Despite being able infect 42% (5 12) control mice, low-dose inoculum failed penetrate...
One of the characteristics prions is their ability to infect some species but not others and prion resistant have been special interest because potential in deciphering determinants for susceptibility. Previously, we developed different vitro vivo models assess susceptibility that were erroneously considered infection, such as members Leporidae Equidae families. Here undertake approaches understand unresolved low canids. Studies based on amino acid sequence canine protein (PrP), together...
Unlike other species, prion disease has never been described in dogs even though they were similarly exposed to the bovine spongiform encephalopathy (BSE) agent. This resistance prompted a thorough analysis of canine PRNP gene and presence negatively charged amino acid residue position 163 was readily identified as potentially fundamental it differed from all known susceptible species. In present study, first transgenic mouse model expressing dog protein (PrP) generated challenged...
In humans, insert mutations within the repetitive octapeptide region of prion protein gene ( Prnp ) are often associated with familial spongiform encephalopathies. this study, transgenic mice expressing bovine PrP (boTg mice) bearing an additional insertion to wild type (seven repeats instead six) showed altered course encephalopathy (BSE) infection, reflected as reduced incubation times when compared boTg similar levels wild-type six-octapeptide protein. both mouse lines (bo6ORTg and...
Transgenic (Tg) mice carrying four extra octapeptide repeats (OR) in the bovine PrP gene (10OR instead of 6) have been generated. In these mice, neuropathological changes were observed depending upon level transgene expression. These primarily involved a slowly advancing neurological disorder, characterized clinically by ataxia, and neuropathologically, vacuolization different brain areas, gliosis, loss cerebellar granule cells. Accumulation insoluble 10OR‐PrP (bo10OR‐PrP) was on expression...
Transmissible spongiform encephalopathies (TSEs) can be ameliorated by prion protein (PrP)-specific antibodies, but active immunization is complicated immune tolerance to the normal cellular host (PrP(C)). Here, we show that DNA of wild-type mice break against protein, resulting in induction PrP-specific antibody and T-cell responses. PrP immunogenicity was increased fusion lysosomal targeting signal from LIMPII (lysosomal integral membrane type II). Although immunized with a PrP-LIMPII...
Prion diseases are characterised by severe neural lesions linked to the presence of an abnormal protease‐resistant isoform cellular prion protein (PrPc). The peptide PrP(106–126) is widely used as a model neurotoxicity in diseases. Here, we examine detail intracellular signalling cascades induced cortical neurons and participation PrPc. We show that induces activation subsets kinases (e.g., ERK1/2), early growth response 1 synthesis caspase‐3 activity, all which mediated nicotinamide adenine...
Esta investigación estudia la relación entre los niveles de conocimiento y gestión costos producción con las variables socio-demográficas gerentes. A partir un estudio cuantitativo, se aplicó instrumento en escala Likert a grupo 21 empresas industria gastronómica, San José Cúcuta, Colombia. Las hipótesis contrastaron través análisis varianza correlación Pearson. Los resultados indican que existe una Conocimientos Materiales Directos Años Antigüedad Igualmente, conocimientos por parte Esto...
RK13 cell lines generated to express bovine PrP(C) with a four extra octarepeat insertional mutation (Bo-10ORPrP(C)) show partially insoluble and lower rates of growth when compared either the same cells expressing wild type Bo-6ORPrP(C) or original line. The expression Bo-10ORPrP(C) in cultures was also associated changes size reorganization actin cytoskeleton. This last process reversed by Clostridium difficile toxin-B, specific inhibitor small GTPase proteins. Further, clones...
We review the current knowledge relative to role of different immune system components and their contribution spread prions throughout infected organism.During last years research made on transmissible spongiform encephalopathies conducted assumption that changes in structure a determined protein (prion protein, PrP) may become self-propagative. The generally assumed finding an abnormally folded as causative agent, able propagate hosts from it was originated, is changing our views diseases....
ABSTRACT Unlike other species, such as cattle, cats or humans, prion disease has never been described in dogs, even though they were similarly exposed to the bovine spongiform encephalopathy (BSE) agent. This resistance prompted a thorough analysis of canine PRNP gene and presence negatively charged amino acid residue position 163 was readily identified potentially fundamental it differed from all known susceptible species. Furthermore, recent results our group demonstrated that mouse with...