A5107632676- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Memory and Neural Mechanisms
- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Genomics and Rare Diseases
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Genomic variations and chromosomal abnormalities
- Cancer Genomics and Diagnostics
- MicroRNA in disease regulation
- Photoreceptor and optogenetics research
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Signaling Pathways in Disease
- Neural dynamics and brain function
- Zebrafish Biomedical Research Applications
- Cell Adhesion Molecules Research
- RNA regulation and disease
- RNA Research and Splicing
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
Biomedical Research Networking Center on Neurodegenerative Diseases
2015-2024
Instituto de Salud Carlos III
2015-2024
Universitat de Barcelona
2015-2024
Centro de Investigación Biomédica en Red
2007-2023
Boston Children's Hospital
2023
Broad Institute
2023
Harvard University
2023
Neurosciences Institute
2022
Columbia University
2022
Vall d'Hebron Institut de Recerca
2014-2020
The reelin gene encodes an extracellular protein that is crucial for neuronal migration in laminated brain regions. To gain insights into the functions of Reelin, we performed high-resolution situ hybridization analyses to determine pattern expression developing forebrain mouse. We also double-labeling studies with several markers, including calcium-binding proteins, GAD65/67, and neuropeptides, characterize subsets express transcripts. was detected at embryonic day 10 later forebrain, a...
Neurons display a highly polarized microtubule network that mediates trafficking throughout the extensive cytoplasm and is crucial for neuronal differentiation function. In newborn migrating neurons, organized by centrosome. During neuron maturation, however, centrosome gradually loses this activity, how microtubules are in more mature neurons remains poorly understood. Here, we demonstrate organization post-mitotic strongly depends on non-centrosomal nucleation mediated augmin nucleator...
DYRK1A is the human orthologue of Drosophila minibrain (mnb) gene, which involved in postembryonic neurogenesis flies.Because its mapping position on chromosome 21 and neurobehavioral alterations shown by mice overexpressing this involvement some neurological defects Down syndrome patients has been suggested.To gain insight into physiological role, we have generated deficient Dyrk1A function gene targeting.Dyrk1A ؊/؊ null mutants presented a general growth delay died during midgestation.Mice...
Spontaneous neural activity is a basic property of the developing brain, which regulates key developmental processes, including migration, differentiation and formation refinement connections. The mechanisms regulating spontaneous are not known. By using transgenic embryos that overexpress BDNF under control nestin promoter, we show here controls emergence robustness in embryonic hippocampal slices. Further, dramatically increases co-active network activity, believed to synchronize gene...
Recent studies have suggested a role for neurotrophins in the growth and refinement of neural connections, dendritic growth, activity-dependent adult plasticity. To unravel endogenous development connections CNS, we studied ontogeny hippocampal afferents trk B (−/−) C mice. Injections lipophilic tracers entorhinal cortex hippocampus newborn mutant mice showed that ingrowth commissural/associational to was not affected by these mutations. Similarly, injections biocytin postnatal (P10–P16) did...
Cortical layers VI to II develop between two of older neurons, the marginal and subplate zones, which are believed have unique roles in cortical development. While cells been found essential for establishment thalamocortical relationships, function zone particular neurons Cajal-Retzius has not elucidated. Here we show that an antibody against calcium-binding protein calretinin labels population throughout their life murine cerebral cortex. In prenatal early postnatal stages, were evenly...
Spontaneous neuronal activity is essential to neural development. Until recently, neurons were believed be the only excitable cells display spontaneous activity. However, cultured astrocytes and, more in situ are now known exhibit Ca2+ transients. Here we used imaging of from transgenic mice for simultaneous monitoring [Ca2+]i changes large numbers astrocytes. We found that a common property most brain lost response lesion. These oscillations require extracellular and intracellular Ca2+....
ABSTRACT Netrin 1 is a long-range diffusible factor that exerts chemoattractive or chemorepulsive effects on developing axons growing to away from the neural midline. Here we used tissue explants study action of netrin in migration several cerebellar and precerebellar cell progenitors. We show strong effect migrating neurons embryonic lower rhombic lip at E12-E14, which give rise nuclei. promotes exit postmitotic upregulates expression TAG-1 these neurons. In addition, presence 1, are not...
Reelin is a glycoprotein that essential for the correct cytoarchitectonic organization of developing CNS. Its function in adult brain less understood, although it has been proposed involved signaling pathways linked to neurodegeneration. Here we analyzed expression brains and cerebrospinal fluid (CSF) from Alzheimer's disease (AD) patients nondemented controls. We found 40% increase protein levels cortex AD compared with Similar increases were detected at mRNA transcriptional level. This...
Reelin, an extracellular protein essential for neural migration and lamination, is also expressed in the adult brain. To unravel function of this forebrain, we generated transgenic mice that overexpress Reelin under control CaMKIIα promoter. Overexpression increased neurogenesis impaired positioning adult-generated neurons. In hippocampus, overexpression resulted increase synaptic contacts hypertrophy dendritic spines. Induction long-term potentiation (LTP) alert-behaving showed evokes a...
Newborn mice carrying targeted mutations in genes encoding neurotrophins or their signaling Trk receptors display severe neuronal deficits the peripheral nervous system but not CNS. In this study, we show that trkB (-/-) have a significant increase apoptotic cell death different regions of brain during early postnatal life. The most affected region is dentate gyrus hippocampus, although elevated levels pyknotic nuclei were also detected cortical layers II and III V VI, striatum, thalamus....
ABSTRACT The semaphorins are the largest family of repulsive axon guidance molecules. Secreted bind neuropilin receptors and repel sensory, sympathetic motor axons. Here we show that CA1, CA3 dentate gyrus axons from E15-E17 mouse embryo explants selectively repelled by entorhinal cortex neocortex. secreted Sema III IV their Neuropilin-1 -2 expressed in hippocampal formation during appropriate stages. strongly axons; only III. An antibody against blocks action cortex, but has no effect on...
We report in this study that, the cerebellum, pancreatic transcription factor Ptf1a is required for specific generation of Purkinje cells (PCs) and interneurons. Moreover, granule cell progenitors external GCL (EGL) appear to be unaffected by deletion . Cell lineage analysis Cre/Cre mice was used establish absence expression, ventricular zone progenitors, normally fated produce PCs interneurons, aberrantly migrate EGL express typical markers these cells, such as Math1, Reelin, Zic1/2....
Abstract We have analyzed the early development of main hippocampal afferents in mouse. Following injections lipophilic tracer 1–1′‐dioctadecyl‐3, 3, 3′, 3′‐tetramethylindocarbocyanine perchorate (DiI) entorhinal cortex, axons were observed for first time inthe hippocampus at E15, white matter, At E17, fibers arborized within stratum lacunosum‐moleculare. subsequent stages formed dense networks that restricted to their appropriate termination zone The invading fascia dentata noticed E19,...