A5018655473- Neurogenesis and neuroplasticity mechanisms
- Neuroscience and Neuropharmacology Research
- Axon Guidance and Neuronal Signaling
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Zebrafish Biomedical Research Applications
- MicroRNA in disease regulation
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Digital Imaging in Medicine
- Electrochemical Analysis and Applications
- Breast Cancer Treatment Studies
- Sleep and Wakefulness Research
- Cannabis and Cannabinoid Research
- Mitochondrial Function and Pathology
- Neurotransmitter Receptor Influence on Behavior
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Nerve injury and regeneration
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Neurofibromatosis and Schwannoma Cases
- Nicotinic Acetylcholine Receptors Study
- Breast Lesions and Carcinomas
Universitat de Barcelona
2005-2021
Barcelona Biomedical Research Park
2004-2016
Biomedical Research Networking Center on Neurodegenerative Diseases
2010-2012
Institute for Research in Biomedicine
2007-2012
Instituto de Salud Carlos III
2008-2011
Écologie Marine Tropicale des Océans Pacifique et Indien
2005
Institut d'Investigacions Biomèdiques de Barcelona
2005
Municipal Institute for Medical Research
2002
Recent studies have suggested a role for neurotrophins in the growth and refinement of neural connections, dendritic growth, activity-dependent adult plasticity. To unravel endogenous development connections CNS, we studied ontogeny hippocampal afferents trk B (−/−) C mice. Injections lipophilic tracers entorhinal cortex hippocampus newborn mutant mice showed that ingrowth commissural/associational to was not affected by these mutations. Similarly, injections biocytin postnatal (P10–P16) did...
Cortical layers VI to II develop between two of older neurons, the marginal and subplate zones, which are believed have unique roles in cortical development. While cells been found essential for establishment thalamocortical relationships, function zone particular neurons Cajal-Retzius has not elucidated. Here we show that an antibody against calcium-binding protein calretinin labels population throughout their life murine cerebral cortex. In prenatal early postnatal stages, were evenly...
Reelin, an extracellular protein essential for neural migration and lamination, is also expressed in the adult brain. To unravel function of this forebrain, we generated transgenic mice that overexpress Reelin under control CaMKIIα promoter. Overexpression increased neurogenesis impaired positioning adult-generated neurons. In hippocampus, overexpression resulted increase synaptic contacts hypertrophy dendritic spines. Induction long-term potentiation (LTP) alert-behaving showed evokes a...
We report in this study that, the cerebellum, pancreatic transcription factor Ptf1a is required for specific generation of Purkinje cells (PCs) and interneurons. Moreover, granule cell progenitors external GCL (EGL) appear to be unaffected by deletion . Cell lineage analysis Cre/Cre mice was used establish absence expression, ventricular zone progenitors, normally fated produce PCs interneurons, aberrantly migrate EGL express typical markers these cells, such as Math1, Reelin, Zic1/2....
Toward understanding topographically specific branching of retinal axons in their target area, we have studied the interaction between neurotrophin receptors and members Eph family. TrkB its ligand BDNF are uniformly expressed retina tectum, respectively, exert a branch-promoting activity, whereas EphAs ephrinAs gradients tectum can mediate suppression axonal branching. We identified novel cis ephrinA5 on ganglion cell axons. interacts with via second cysteine-rich domain (CC2), which is...
Here we examine the role of Reelin, an extracellular protein involved in neuronal migration, formation hippocampal connections. Both at prenatal and postnatal stages, general laminar topographic distribution entorhinal projections is preserved hippocampus reeler mutant mice, absence Reelin. However, developing adult afferents show severe alterations, including increased numbers misrouted fibers abnormal patches termination from medial lateral cortices. At perinatal single axons mice are...
During neural development, specific recognition molecules provide the cues necessary for formation of initial projection maps, which are reshaped later in development. In some systems, guiding axonal pathfinding and target selection provided by cells that present only at critical times. For instance, floor plate guides commissural axons spinal cord, subplate is involved thalamocortical connections. Here we study development entorhinal connections to murine hippocampus, adult terminate...
Abstract Immunocytochemical techniques were used to characterize the neuronal populations in hippocampal subplate and marginal zone from embryonic day 13 (E13) postnatal 5 (P5). Sections processed for visualization of microtubule‐associated protein 2 (MAP2) other antigens such as neurotransmitters, neuropeptides, calcium‐binding proteins a synaptic antigen (Mab SMI81). At E13–E14, only ventricular primitive plexiform layer recognized. Some cells later stratum displayed MAP2‐, γ‐aminobutyric...
Despite the impact of schizophrenia and mood disorders, which in extreme cases can lead to death, recent decades have brought little progress development new treatments. Recent studies shown that Reelin, an extracellular protein is critical for neuronal development, reduced bipolar disorder patients. However, data on a causal or protective role Reelin psychiatric diseases scarce. In order study direct influence Reelin's levels behavior, we subjected two mouse lines, are either (Reelin...
Patients with Alzheimer's disease (AD) display altered functioning of cortical networks, including patterns synchronous activity and a serious deficit in cholinergic septohippocampal (SH) innervation. However, the mechanisms underlying these alterations implication GABAergic SH component AD are largely unknown. In addition, pathway (SHP) is believed to regulate hippocampal by controlling interneurons. Here we show, using well-characterized tracing experiments, that innervation SHP decreases...
Adult hippocampal neurogenesis is thought to be essential for learning and memory, has been implicated in the pathogenesis of several disorders. Although recent studies have identified key factors regulating neuroprogenitor proliferation adult hippocampus, mechanisms that control migration integration adult-born neurons into circuits are largely unknown. Reelin an extracellular matrix protein vital neuronal development. Activation cascade leads phosphorylation Disabled-1, adaptor required...
Abstract The prenatal and postnatal development of calretinin (CR)‐containing elements in the neocortex rat was analyzed using immunohistochemistry. CR immunoreactivity cortical anlage appeared early at embryonic day 14 (E14), with CR‐positive neurons located primitive plexiform layer emerging subplate marginal zones. At later stages, these two layers showed highest immunostaining cortex, large numbers cell bodies fibers were immunostained. From 3 (P3) onwards, disappeared progressively from...
Entorhinal fibers to the fascia dentata originating from layer II stellate neurons are known terminate exclusively in outer two thirds of molecular layer, where they innervate distal dendritic portions dentate neurons. Using anterograde tracing with Phaseolus vulgaris leucoagglutinin, we unraveled a previously unknown entorhinal projection that directly innervates proximal and somata granule cells GABAergic This originates located layers IV-VI medial area. These enter traverse inner (IML)...
Mutations in the MLC1 gene are responsible for one form of neurological disorder megalencephalic leukoencephalopathy with subcortical cysts (MLC). The disease is a type vacuolating myelinopathy. biochemical properties and function protein unknown. To characterize MLC1, we generated polyclonal antibodies. was detected brain, assembled into higher molecular complexes, as assessed by assembly-dependent trafficking assays. In situ hybridization immunohistochemistry were used to determine...
Neural development and plasticity are regulated by neural adhesion proteins, including the polysialylated form of NCAM (PSA-NCAM). Podocalyxin (PC) is a renal PSA-containing protein that has been reported to function as an anti-adhesin in kidney podocytes. Here we show PC widely expressed neurons during development. interacts with ERM family, NHERF1/2 RhoA/G. Experiments vitro phenotypic analyses podxl-deficient mice indicate involved neurite growth, branching axonal fasciculation,...
The extracellular protein Reelin has an important role in neurological diseases, including epilepsy, Alzheimer's disease and psychiatric targeting hippocampal circuits. Here we address the of development synaptic contacts adult-generated granule cells (GCs), a neuronal population that is crucial for learning memory implicated diseases. We found pathway controls shapes, sizes, types dendritic spines, complexity multisynaptic innervations degree perisynaptic astroglial ensheathment...
Megalencephalic leukoencephalopathy with subcortical cysts (MLC) is a rare type of leukodystrophy, most often caused by mutations in the MLC1 gene. an oligomeric plasma membrane (PM) protein unknown function expressed mainly glial cells and neurons. Most disease-causing missense dramatically reduced total PM expression levels Xenopus oocytes mammalian cells. The impaired mutants was verified primary cultures rat astrocytes, as well human monocytes, cell types that endogenously express MLC1,...
Patterned intrinsic network activity plays a central role in shaping immature neuronal networks into functional circuits.However, the long-lasting signals that regulate spontaneous of developing circuits have not been identified.Here we study net impact TrkB signaling on early identified populations by analyzing postnatal hippocampi from trkB null mice.Ca 21 imaging showed pyramidal neurons 2/2 mice displayed decrease synchronous neonatal animals but an increase juveniles.Strikingly,...