A5004526076- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Mass Spectrometry Techniques and Applications
- ATP Synthase and ATPases Research
- Cellular transport and secretion
- Advanced Proteomics Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Ion Transport and Channel Regulation
- Lipid Membrane Structure and Behavior
- Metabolomics and Mass Spectrometry Studies
- Receptor Mechanisms and Signaling
- Calcium signaling and nucleotide metabolism
- Retinal Development and Disorders
- Magnesium in Health and Disease
- Ion Channels and Receptors
- Cardiac Ischemia and Reperfusion
- Pancreatic function and diabetes
- Hearing, Cochlea, Tinnitus, Genetics
- Electrochemical Analysis and Applications
- Trypanosoma species research and implications
University of Freiburg
2015-2025
Logopharm (Germany)
2012-2023
Czech Academy of Sciences, Institute of Physiology
2015-2023
Innsbruck Medical University
2006
University of Tübingen
1996-2002
Philipps University of Marburg
1999
Adenosine triphosphate (ATP)–sensitive potassium (K ATP ) channels couple electrical activity to cellular metabolism through their inhibition by intracellular ATP. of K varies among tissues and is affected the metabolic regulatory state individual cells, suggesting involvement endogenous factors. It reported here that phosphatidylinositol-4,5-bisphosphate (PIP 2 phosphatidylinositol-4-phosphate (PIP) controlled cloned ir 6.2 SUR1). These phospholipids acted on subunit shifted sensitivity...
Outer hair cells (OHCs) of the mammalian cochlea actively change their cell length in response to changes membrane potential. This electromotility, thought be basis cochlear amplification, is mediated by a voltage-sensitive motor molecule recently identified as protein prestin. Here, we show that voltage sensitivity conferred prestin intracellular anions chloride and bicarbonate. Removal these abolished fast voltage-dependent motility, well characteristic nonlinear charge movement (“gating...
Large-conductance calcium- and voltage-activated potassium channels (BK Ca ) are dually activated by membrane depolarization elevation of cytosolic calcium ions (Ca 2+ ). Under normal cellular conditions, BK channel activation requires concentrations that typically occur in close proximity to sources. We show affinity-purified from rat brain assembled into macromolecular complexes with the voltage-gated Cav1.2 (L-type), Cav2.1 (P/Q-type), Cav2.2 (N-type). Heterologously expressed -Cav...
Glutamate receptors of the AMPA-subtype (AMPARs), together with transmembrane AMPAR regulatory proteins (TARPs), mediate fast excitatory synaptic transmission in mammalian brain. Here, we show by proteomic analysis that majority AMPARs rat brain are coassembled two members cornichon family proteins, rather than TARPs. Coassembly homologs 2 and 3 affects ways: Cornichons increase surface expression AMPARs, they alter channel gating markedly slowing deactivation desensitization kinetics. These...
Local Ca(2+) signaling occurring within nanometers of voltage-gated (Cav) channels is crucial for CNS function, yet the molecular composition Cav channel nano-environments largely unresolved. Here, we used a proteomic strategy combining knockout-controlled multiepitope affinity purifications with high-resolution quantitative MS comprehensive analysis Cav2 family in whole rodent brain. The shows that channels, composed pore-forming alpha1 and auxiliary beta subunits, are embedded into protein...
Mitochondria have crucial roles in cellular energetics, metabolism, signalling and quality control
Article13 November 2020Open Access Source DataTransparent process Flavonol-mediated stabilization of PIN efflux complexes regulates polar auxin transport William D Teale Corresponding Author [email protected] orcid.org/0000-0003-0956-7372 Institute Biology II, University Freiburg, Germany Search for more papers by this author Taras Pasternak Cristina Dal Bosco Alexander Dovzhenko orcid.org/0000-0002-9940-3074 Krystyna Kratzat Wolfgang Bildl Physiology Faculty Medicine, Manuel Schwörer...
Inward-rectifier K(+) channels of the ROMK (K(ir)1.1) subtype are responsible for secretion and control NaCl absorption in kidney. A hallmark these is their gating by intracellular pH neutral range. Here we show that a lysine residue close to TM1, identified previously as structural element required pH-induced gating, protonated at this protonation drives other K(ir) channels. Such anomalous titration (Lys-80 K(ir)1.1) accomplished tertiary structure protein: two arginines distant N C...
Niemann-Pick C1-like protein (NPC1L1) mediates the absorption of dietary cholesterol in proximal region intestine, a process that is blocked by inhibitors (CAIs), including ezetimibe (EZE). Using proteomic approach, we demonstrate NPC1L1 to which EZE and its analogs bind. Next, determined site interaction with exploiting different binding affinities mouse dog for radioligand analog EZE, [(3)H]AS. Chimeric mutational studies indicate high-affinity [(3)H]AS depends on molecular determinants...
The encoding of auditory information with indefatigable precision requires efficient resupply vesicles at inner hair cell (IHC) ribbon synapses. Otoferlin, a transmembrane protein responsible for deafness in DFNB9 families, has been postulated to act as calcium sensor exocytosis well be involved rapid vesicle replenishment IHCs. However, the molecular basis recycling IHCs is largely unknown. In present study, we used high-resolution liquid chromatography coupled mass spectrometry copurify...