A5075670973- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Nicotinic Acetylcholine Receptors Study
- Calcium signaling and nucleotide metabolism
- Neuroscience and Neural Engineering
- Ion Channels and Receptors
- Receptor Mechanisms and Signaling
- Adenosine and Purinergic Signaling
- Cellular transport and secretion
- Neonatal Health and Biochemistry
- Genomics and Phylogenetic Studies
- Pancreatic function and diabetes
- RNA Research and Splicing
- Cardiomyopathy and Myosin Studies
- RNA and protein synthesis mechanisms
- Neuropeptides and Animal Physiology
- Trace Elements in Health
- Probiotics and Fermented Foods
- Metabolomics and Mass Spectrometry Studies
- Ion Transport and Channel Regulation
- Advanced Proteomics Techniques and Applications
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Memory and Neural Mechanisms
University of Freiburg
2014-2025
Technical University of Munich
1996-2006
Logopharm (Germany)
2006
Innsbruck Medical University
2006
University of Leeds
2004
Ludwig-Maximilians-Universität München
1995-2002
Deutsche Forschungsgemeinschaft
2002
Mount Desert Island Biological Laboratory
2002
Boehringer Ingelheim (India)
2002
Saitama Medical University
2000
Ebola virus causes sporadic outbreaks of lethal hemorrhagic fever in humans, but there is no currently approved therapy. Cells take up by macropinocytosis, followed trafficking through endosomal vesicles. However, few factors controlling movement are known. Here we find that entry into host cells requires the calcium channels called two-pore (TPCs). Disrupting TPC function gene knockout, small interfering RNAs, or small-molecule inhibitors halted and prevented infection. Tetrandrine, most...
Current knowledge about the molecular mechanisms of NMDA receptor (NMDAR)-independent long-term potentiation (LTP) in hippocampus and its function for memory formation behaving animal is limited. NMDAR-independent LTP CA1 region thought to require activity postsynaptic L-type voltage-dependent Ca 2+ channels (Ca v 1.x), but underlying channel isoform remains unknown. We evaluated 1.2 spatial learning, synaptic plasticity, triggering learning-associated biochemical processes using a mouse...
Large-conductance calcium- and voltage-activated potassium channels (BK Ca ) are dually activated by membrane depolarization elevation of cytosolic calcium ions (Ca 2+ ). Under normal cellular conditions, BK channel activation requires concentrations that typically occur in close proximity to sources. We show affinity-purified from rat brain assembled into macromolecular complexes with the voltage-gated Cav1.2 (L-type), Cav2.1 (P/Q-type), Cav2.2 (N-type). Heterologously expressed -Cav...
Sequence database searches with the alpha2delta subunit as probe led to identification of two new genes encoding proteins essential properties this calcium channel subunit. Primary structure comparisons revealed that novel alpha2delta-2 and alpha2delta-3 subunits share 55.6 30.3% identity alpha2delta-1 subunit, respectively. The number putative glycosylation sites cysteine residues, hydropathicity profiles, electrophysiological character indicates these are functional subunits. Coexpression...
High-voltage activated calcium channels are modulated by a series of auxiliary proteins, including those the α<sub>2</sub>δ family. Until recently, only single subunit was known, but two further members, α<sub>2</sub>δ-2 and -3, have since been identified. In this study, structure these novel subunits has characterized binding antiepileptic drug gabapentin investigated. Using antibodies directed against amino terminal portion gross could be analyzed Western blotting. Similar to...
Dihydropyridines (DHPs) block the vascular smooth muscle L-type Ca2+ channel at lower concentrations than cardiac channel, although their alpha 1 subunit, which binds DHPs, is derived from same gene. This 1C gene gives rise to several splice variants, among 1C-b variant affected by of nisoldipine 1C-a variant. Functional expression chimeras and subunits demonstrated that transmembrane segment IS6 responsible for different dihydropyridine sensitivity. Northern blot analysis showed transcripts...
Cyclic nucleotide-gated cation channels are essential in visual and olfactory signal transduction. An additional member of the cGMP-gated channel family, termed CNG-3, has been cloned from bovine kidney. Its deduced amino acid sequence is 60% 62% identical with CNG-channel proteins rod outer segment epithelium, respectively. Northern analysis sequences amplified by PCR showed that CNG-3 mRNA present testis, kidney, heart. Calcium permeated expressed presence extracellular Mg2+ Na+ at...
Recent clinical data support the use of oral lavender oil in patients suffering from subsyndromal anxiety. We identified molecular mechanism action that will alter perception as a nonspecific ingredient aromatherapy to potent anxiolytic inhibiting voltage dependent calcium channels (VOCCs) highly selective drug target. In contrast previous publications where exorbitant high concentrations were used, effects behavioral, biochemical, and electrophysiological experiments investigated...
The γ subunit was initially identified as an auxiliary of the skeletal muscle calcium channel complex. Evidence for existence further subunits arose following characterization a genetic defect that induces epileptic seizures in stargazer mice. We present here first account family at least five putative are predominantly expressed brain. γ‐2 and γ‐4 shift steady‐state inactivation curve to more hyperpolarized potentials upon coexpression with P/Q type α 1A subunit. γ‐5 accelerates time course...
Calcium-dependent facilitation of L-type calcium channels has been reported to depend on the function calmodulin kinase II. In contrast, mechanism for voltage-dependent is not clear. HEK 293 cells expressing Ca(v)1.2, Ca(v)beta2a, and II, current measured at +30 mV was facilitated up 1.5-fold by a 200-ms-long prepulse +160 mV. This prevented II inhibitors KN93 autocamtide-2-related peptide. Ca(v)1.2 mutation I1649E, residue critical binding Ca2+-bound calmodulin, also abolished. Calmodulin...
Abstract The auxiliary calcium channel α 2 δ subunit comprises a family of three genes, δ‐1 to 3, which are expressed in tissue‐specific manner. δ‐2 mRNA is found the heart, skeletal muscle, brain, kidney, liver and pancreas. We report here for first time identification functional characterization splice variants their distribution mouse brain. differ protein by eight amino acid residues, respectively, differentially cardiac tissue human medullary thyroid carcinoma (hMTC) cells. In situ...
ABSTRACT Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy those 8 after were examined for Borrelia burgdorferi DNA by PCR. 27 dermatological diseases other than Lyme borreliosis 10 healthy persons as controls. Two different primer sets targeting 23S rRNA (PCR I) 66-kDa protein II) genes used. PCR was performed freshly frozen tissue (FFT) paraffin-embedded (PET). For FFT specimens migrans, 73% positive I, 79% II, 88% combining I...
ABSTRACT Mice deficient in the smooth muscle Ca v 1.2 calcium channel (SMACKO, α 1c ‐ subunit knockout) have a severely reduced micturition and an increased bladder mass. L‐type current, protein, spontaneous contractile activity were absent of SMACKO mice. K + carbachol (CCh)‐induced contractions to 10‐ fold detrusor muscles from The dihydropyridine isradipine inhibited CCh‐induced CTR but had no effect contraction was blocked by removing extracellular 2+ unaffected PLC inhibitor U73122 or...