A5009279426- Calcium signaling and nucleotide metabolism
- Glycosylation and Glycoproteins Research
- Proteoglycans and glycosaminoglycans research
- Ion Channels and Receptors
- Bone Metabolism and Diseases
- Electromagnetic Compatibility and Noise Suppression
- Cellular transport and secretion
- Mobile Ad Hoc Networks
- Carbohydrate Chemistry and Synthesis
- Organic Light-Emitting Diodes Research
- Phytochemistry and Biological Activities
- Adenosine and Purinergic Signaling
- Piperaceae Chemical and Biological Studies
- Photonic Crystals and Applications
- GaN-based semiconductor devices and materials
- Natural product bioactivities and synthesis
- Photonic and Optical Devices
- Energy Efficient Wireless Sensor Networks
- Microwave Engineering and Waveguides
- Microwave and Dielectric Measurement Techniques
- Opportunistic and Delay-Tolerant Networks
- Electromagnetic Compatibility and Measurements
- Organic Electronics and Photovoltaics
- Energy Harvesting in Wireless Networks
- Electrostatic Discharge in Electronics
National Taiwan University
2008-2025
National Taiwan University Hospital
2022-2025
Bureau of Standards, Metrology and Inspection
2006-2022
Ludwig-Maximilians-Universität München
2012-2022
Chang Gung Memorial Hospital
2020-2021
ITRI International
2013-2021
Center for Integrated Protein Science Munich
2014-2018
Industrial Technology Research Institute
2013-2017
National Taiwan University of Science and Technology
2010-2017
Tamkang University
2009-2017
Ebola virus causes sporadic outbreaks of lethal hemorrhagic fever in humans, but there is no currently approved therapy. Cells take up by macropinocytosis, followed trafficking through endosomal vesicles. However, few factors controlling movement are known. Here we find that entry into host cells requires the calcium channels called two-pore (TPCs). Disrupting TPC function gene knockout, small interfering RNAs, or small-molecule inhibitors halted and prevented infection. Tetrandrine, most...
Article14 April 2015Open Access Source Data Expression of Ca2+-permeable two-pore channels rescues NAADP signalling in TPC-deficient cells Margarida Ruas Department Pharmacology, University Oxford, UK Search for more papers by this author Lianne C Davis Cheng-Chang Chen Center Integrated Protein Science CIPS-M and Pharmacy – Drug Research, Ludwig-Maximilians-Universität München, Germany Anthony J Morgan Kai-Ting Chuang Timothy F Walseth Pharmacology Department, Minnesota, Minneapolis, MN,...
Ion selectivity is a defining feature of given ion channel and considered immutable. Here we show that the lysosomal TPC2, which hotly debated (Calcraft et al., 2009; Guo 2017; Jha 2014; Ruas 2015; Wang 2012), depends on activating ligand. A high-throughput screen identified two structurally distinct TPC2 agonists. One these evoked robust Ca2+-signals non-selective cation currents, other weaker Na+-selective currents. These properties were mirrored by Ca2+-mobilizing messenger, NAADP...
Cytokines and chemokines are produced secreted by a broad range of immune cells including macrophages. Remarkably, little is known about how these inflammatory mediators released from the various cells. Here, endolysosomal cation channel TRPML2 shown to play direct role in chemokine trafficking secretion murine To demonstrate acute involvement processes, first isoform-selective agonist was generated, ML2-SA1. ML2-SA1 not only found directly stimulate release CCL2 macrophages but also...
Lung emphysema and chronic bronchitis are the two most common causes of obstructive pulmonary disease. Excess macrophage elastase MMP-12, which is predominantly secreted from alveolar macrophages, known to mediate development lung injury emphysema. Here, we discovered endolysosomal cation channel mucolipin 3 (TRPML3) as a regulator MMP-12 reuptake broncho-alveolar fluid, driving in independently generated Trpml3-/- mouse models enlarged injury, further exacerbated after or tobacco smoke...
Yellow emission is crucial in RGBY display technology and fabricating physiologically friendly, low color‐temperature lighting sources. Emitters with both wet‐ dry‐process feasibility are highly desirable to fabricate, respectively, high‐quality devices via vapor deposition cost‐effective, large‐area roll‐to‐roll fabrication. Here, high‐efficiency organic light‐emitting diodes a novel feasible yellow‐emitting iridium complex, bis[5‐methyl‐7‐fluoro‐5H‐benzo(c)(1,5) naphthyridin‐6‐one]iridium...
Significance Polymorphisms in the endolysosomal cation channel TPC2 have been suggested to lead a shift human hair color from brown blond. In two further studies role for melanosomal pH regulation was postulated. Electrophysiological data on how these polymorphisms affect gating and activity are, however, missing. We show here that both gain of function by different mechanisms. M484L sensitivity its endogenous ligand PI(3,5)P 2 is strongly increased while G734E inactivation ATP reduced....
Two-pore channel 2 (TPC2) resides in endolysosomal membranes but also lysosome-related organelles such as the melanin producing melanosomes. Gain-of-function polymorphisms hTPC2 are associated with decreased production and blond hair color. Vice versa genetic ablation of TPC2 increases production. We show here an inverse correlation between melanoma proliferation, migration, invasion due to dual activity endolysosomes Our results supported by both pharmacological inhibition TPC2....
In light of the remarkable benefits and numerous applications Takagi-Sugeno (T-S) fuzzy system modeling method sliding mode control (SMC) technique, this paper aims to study design robust controllers for a set second-order systems using combination these two approaches. The combined scheme is shown have merits both It alleviates not only online computational burden by T-S model approximate original nonlinear one (since most parameters can be computed offline) but also preserves advantages...
Activation of hyperpolarization-activated cyclic nucleotide-gated (HCN) channels is facilitated in vivo by direct binding the second messenger cAMP. This process plays a fundamental role fine-tuning HCN channel activity and critical for modulation cardiac neuronal rhythmicity. Here, we identify pyrimidine nucleotide cCMP as another regulator channels. We demonstrate that shifts activation curves two members family, HCN2 HCN4, to more depolarized voltages. Moreover, speeds up slows down...
Mucolipidosis type IV (MLIV) is caused by loss of function mutations in the TRPML1 ion channel. We previously reported that tissue zinc levels MLIV were abnormally elevated; however, mechanism behind this pathologic accumulation remains unknown. Here, we identify transmembrane (TMEM)-163 protein, a putative transporter, as novel interacting partner for TRPML1. Evidence from yeast two-hybrid, expression pattern, co-immunoprecipitation, mass spectrometry and confocal microscopy studies...
Endolysosomes are dynamic, intracellular compartments, regulating their surface-to-volume ratios to counteract membrane swelling or shrinkage caused by osmotic challenges upon tubulation and vesiculation events. While osmosensitivity has been extensively described on the plasma membrane, mechanisms underlying endolysosomal ratio changes identities of involved ion channels remain elusive. mediate endocytosis, exocytosis, cargo transport, sorting material for recycling degradation. We...
Abstract Melanoma arising from pigment-producing melanocytes is the deadliest form of skin cancer. Extensive ultraviolet light exposure a major cause melanoma and individuals with low levels melanin are at particular risk. Humans carrying gain-of-function polymorphisms in melanosomal/endolysosomal two-pore cation channel TPC2 present hypopigmentation, blond hair, albinism. Loss associated decreased cancer/melanoma proliferation, migration, invasion, tumor growth metastasis formation,...
Abstract Osteosarcoma is an aggressive bone malignancy with a high propensity for drug resistance and metastasis, leading to poor clinical outcomes. This study investigates the role of core 1 β1,3‐galactosyltransferase (C1GALT1) in osteosarcoma, focusing on its implications chemoresistance. Our findings reveal that expression C1GALT1 associated advanced stages, adverse overall survival, increased recurrence rates. Elevated levels were observed doxorubicin‐selected osteosarcoma cells, where...