A5085038619- Glycosylation and Glycoproteins Research
- Bone Metabolism and Diseases
- Proteoglycans and glycosaminoglycans research
- Systemic Sclerosis and Related Diseases
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Bone and Joint Diseases
- Wnt/β-catenin signaling in development and cancer
- Rheumatoid Arthritis Research and Therapies
- Autophagy in Disease and Therapy
- Osteoarthritis Treatment and Mechanisms
- Dupuytren's Contracture and Treatments
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Hedgehog Signaling Pathway Studies
- MicroRNA in disease regulation
- Connective Tissue Growth Factor Research
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Cancer Diagnosis and Treatment
- Dermatologic Treatments and Research
- Cancer-related molecular mechanisms research
- Fibroblast Growth Factor Research
- Tropical and Extratropical Cyclones Research
National Taiwan University
2006-2025
First People's Hospital of Yuhang District
2025
Friedrich-Alexander-Universität Erlangen-Nürnberg
2012-2024
Universitätsklinikum Erlangen
2017-2024
Yangon Technological University
2024
Hangzhou First People's Hospital
2023
Zhejiang University
2023
<h3>Objectives</h3> Autophagy is a homeostatic process to recycle dispensable and damaged cell organelles. Dysregulation of autophagic pathways has recently been implicated in the pathogenesis various diseases. Here, we investigated role autophagy during joint destruction arthritis. <h3>Methods</h3> osteoclasts was analysed vitro ex vivo by transmission electron microscopy, Western blotting immunohistochemistry for Beclin1 Atg7. Small molecule inhibitors, LysMCre-mediated knockout Atg7...
<h3>Objectives</h3> Activated Wnt signalling with decreased expression of endogenous inhibitors has recently been characterised as a central pathomechanism in systemic sclerosis (SSc). Aberrant epigenetic modifications also contribute to the persistent activation SSc fibroblasts. We investigated whether increased and changes are causally linked via promoter hypermethylation-induced silencing antagonists. <h3>Methods</h3> The methylation status antagonists leucocytes fibroblasts was evaluated...
Osteoarthritis is the most common form of arthritis and a major socioeconomic burden. Our study first to explore association between serum microRNA levels development severe osteoarthritis knee hip joint in general population.
Objectives Autophagy has recently been shown to regulate osteoclast activity and differentiation. Here, we aim investigate the impact of autophagy inhibition as a potential therapeutic approach for treatment osteoporosis in preclinical models. Methods Systemic bone loss was induced mice by glucocorticoids ovariectomy (OVX). targeted conditional inactivation autophagy-related gene 7 (Atg7) with chloroquine (CQ). Bone density evaluated microCT. The role on osteoclastogenesis analysed...
Background and objectives Fibrosis is a major socioeconomic burden, but effective antifibrotic therapies are not available in the clinical routine. There growing evidence for central role of Wnt signalling fibrotic diseases such as systemic sclerosis, we therefore evaluated translational potential pharmacological inhibition experimental dermal fibrosis. Methods We examined effects PKF118-310 ICG-001, two novel inhibitors downstream canonical signalling, models prevention treatment...
Abstract Activation of fibroblasts is essential for physiological tissue repair. Uncontrolled activation fibroblasts, however, may lead to fibrosis with organ dysfunction. Although several pathways capable promoting fibroblast and repair have been identified, their interplay in the context chronic fibrotic diseases remains incompletely understood. Here, we provide evidence that transforming growth factor-β (TGFβ) activates autophagy by an epigenetic mechanism amplify its profibrotic effects....
Transforming growth factor β (TGF-β) signaling is a core pathway of fibrosis, but the molecular regulation activation latent TGF-β remains incompletely understood. Here, we demonstrate crucial role WNT5A/JNK/ROCK that rapidly coordinates in fibrotic diseases. WNT5A was identified as predominant noncanonical WNT ligand diseases such systemic sclerosis, sclerodermatous chronic graft-versus-host disease, and idiopathic pulmonary stimulating fibroblast-to-myofibroblast transition tissue fibrosis...
<h3>Objectives</h3> Canonical Wnt signalling has recently emerged as a key mediator of fibroblast activation and tissue fibrosis in systemic sclerosis. Here, we investigated tankyrases novel molecular targets for inhibition canonical fibrotic diseases. <h3>Methods</h3> The antifibrotic effects the tankyrase inhibitor XAV-939 or siRNA-mediated knockdown were evaluated mouse models bleomycin-induced dermal experimental induced by adenoviral overexpression constitutively active TGF-β receptor I...
<h3>Objectives</h3> Casein kinase II (CK2) is a constitutively active serine/threonine protein that plays key role in cellular transformation and tumorigenesis. The purpose of the study was to characterise whether CK2 contributes pathologic activation fibroblasts patients with SSc evaluate antifibrotic potential inhibition. <h3>Methods</h3> Activation CK2, JAK2 STAT3 human skin experimental fibrosis analysed by immunohistochemistry. signalling inhibited selective inhibitor 4, 5, 6,...
Objectives Notch ligands and receptors have recently been shown to be differentially expressed in osteoarthritis (OA). We aim further elucidate the functional role of signalling OA using Notch1 antisense transgenic (Notch1 AS) mice. Methods hedgehog were analysed by real-time PCR immunohistochemistry. Notch-1 AS mice employed as a model impaired vivo. Experimental was induced destabilisation medial meniscus (DMM). The extent cartilage destruction osteophyte formation safranin-O staining with...
Bone mass is maintained by the balance between osteoclast-induced bone resorption and osteoblast-triggered formation. In inflammatory arthritis such as rheumatoid (RA), however, increased osteoclast differentiation activity skew this resulting in progressive loss. O-GlcNAcylation a posttranslational modification with attachment of single O-linked β-D-N-acetylglucosamine (O-GlcNAc) residue to serine or threonine residues target proteins. Although one most common protein modifications, its...
Abstract Osteosarcoma is an aggressive bone malignancy with a high propensity for drug resistance and metastasis, leading to poor clinical outcomes. This study investigates the role of core 1 β1,3‐galactosyltransferase (C1GALT1) in osteosarcoma, focusing on its implications chemoresistance. Our findings reveal that expression C1GALT1 associated advanced stages, adverse overall survival, increased recurrence rates. Elevated levels were observed doxorubicin‐selected osteosarcoma cells, where...
We probed the roles of SNHG7, miR-146b, PCBP1, and IL-β in development osteoarthritis (OA). OA models were established using anterior cruciate ligaments, chondrocytes obtained from mouse cartilage tissue. Cells treated with 10 ng/ml Il-1β. RT-qPCR was used to detect expression tissues cells. Safranin-O/Fast Green staining performed analyze damage each group mice. SNHG7 PCBP1 expressions down-regulated, miR-146b up-regulated tissue IL-1β-treated compared normal chondrocytes. Forced improved...
<h3>Objectives</h3> The morphogen pathways Hedgehog, Wnt and Notch are attractive targets for antifibrotic therapies in systemic sclerosis. Interference with stem cell regeneration, however, may complicate the use of pathway inhibitors. We therefore tested hypothesis that combination low doses inhibitors maybe safe effective treatment fibrosis. <h3>Methods</h3> Skin fibrosis was induced by bleomycin overexpression a constitutively active TGF-β receptor type I. Adverse events were assessed...
Abstract The glycoprotein CD44 is a key regulator of malignant behaviors in breast cancer cells. To date, hyaluronic acid (HA)-CD44 signaling pathway has been widely documented the context metastatic bone diseases. Core 1 β1,3-galactosyltransferase (C1GALT1) critical enzyme responsible for elongation O-glycosylation. Aberrant O-glycans recognized as hallmark cancers. However, effects C1GALT1 on and metastasis remain unclear. In this study, IHC analysis indicated that expression positively...
Cells in the tumor microenvironment (TME) communicate via membrane‐bound and secreted proteins, which are mostly glycosylated. Altered glycomes of malignant tumors influence behaviors stromal cells. In this study, we showed that loss core‐1 β1,3‐galactosyltransferase (C1GALT1)‐mediated O‐glycosylation suppressed growth syngeneic head neck cancer mouse models. O‐glycan truncation cells promoted M1 polarization macrophages, enhanced T‐cell‐mediated cytotoxicity, reduced interleukin‐6 (IL‐6)...
Abstract Expression of T antigen (Galbeta1, 3GalNAc) is associated with enhanced metastatic potential and poor prognosis in colorectal cancer. Cosmc a molecular chaperone required for the formation an active T‐synthase, which catalyzes synthesis antigen. However, expression role cancer are still unclear. Here, real‐time PCR showed that overexpression mRNA tumors compared paired non‐tumorous tissues was increased American Joint Committee on Cancer (AJCC) tumor stage. Forced HCT116 cells...
Abstract The enzyme β1,4-N-acetylgalactosaminyltransferase III (β4GalNAc-T3) exhibits in vitro activity of synthesizing N,N′-diacetyllactosediamine, GalNAcβ1,4GlcNAc. Here, we investigate the expression β4GalNAc-T3 primary colon tumors and effects its overexpression on HCT116 cancer cells. Real-time reverse transcription-PCR showed that was up-regulated 72.5% (n = 40) compared with their normal counterparts. resulted enhanced cell-extracellular matrix adhesion, migration,...
Preeclampsia is a pregnancy-specific disorder that features insufficient extravillous trophoblast (EVT) invasion. We have previously shown MUC1 expression in human placenta increases with gestational age and inhibits choriocarcinoma cell invasion.Here, we studied whether preeclamptic placentas dysregulated the mechanism of EVT invasion regulated by MUC1.MUC1 severe age-matched control was analyzed real-time RT-PCR, Western blot analysis, immunohistochemistry. The effects on cell-matrix...
Canonical as well non-canonical Wnt signalling pathways have emerged core of fibrosis. Their profibrotic effects are mediated via distinct intracellular cascades independently each other. Thus, inhibition both may additive antifibrotic effects. Here, we knocked down evenness interrupted (EVI) to simultaneously target for the first time canonical and in experimental fibrosis.The siRNA-mediated knockdown EVI were evaluated mouse models bleomycin-induced skin fibrosis induced by adenoviral...
GalNAc‐type O‐glycosylation and its initiating GalNAc transferases (GALNTs) play crucial roles in a wide range of cellular behaviors. Among 20 GALNT members, GALNT2 is consistently associated with poor survival patients colorectal cancer public databases. However, clinicopathological significance remains unclear. In this study, immunohistochemistry showed that was overexpressed tumors compared the adjacent nontumor tissues. overexpression patients. Forced expression promoted migration...