A5077225109- Hearing, Cochlea, Tinnitus, Genetics
- Hearing Loss and Rehabilitation
- Vestibular and auditory disorders
- Neuroscience and Neuropharmacology Research
- Ion Channels and Receptors
- Ion channel regulation and function
- Biochemical Analysis and Sensing Techniques
- Nerve injury and regeneration
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Noise Effects and Management
- Acoustic Wave Phenomena Research
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Connexins and lens biology
- RNA regulation and disease
- Antimicrobial Peptides and Activities
- Mitochondrial Function and Pathology
- Neuroscience of respiration and sleep
- Cell Adhesion Molecules Research
- Multisensory perception and integration
- Insect and Pesticide Research
- Nicotinic Acetylcholine Receptors Study
- Lipid Membrane Structure and Behavior
- Cellular Mechanics and Interactions
University of Tübingen
2016-2025
Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde
2019-2024
Universitätsklinikum Tübingen
2009-2024
Klinik und Poliklinik für Hals-Nasen-Ohren-Heilkunde
2020
Bayer (Germany)
2017
University of Würzburg
2017
University of Cologne
2016
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2015
Bernstein Center for Computational Neuroscience Tübingen
1994-2012
TH Bingen University of Applied Sciences
2005-2012
Abstract In the rat hippocampus, nerve growth factor (NGF) and brain‐derived neurotrophic (BDNF) are synthesized by neurons in an activity‐dependent manner. Glutamate receptor activation increases whereas GABAergic stimulation decreases NGF BDNF mRNA levels. Here we demonstrate that protein up‐regulated hippocampus of muscarinic receptors. Conversely, enhance release acetylcholine (ACh) from hippocampal synaptosomes containing endings septal cholinergic neurons. also rapidly high‐affinity...
Autosomal dominant optic atrophy (adOA) is a juvenile onset, progressive ocular disorder characterized by bilateral loss of vision, central visual field defects, colour vision disturbances, and disc pallor. adOA most frequently associated with mutations in OPA1 encoding dynamin-related large GTPase that localizes to mitochondria. Histopathological studies patients have shown degeneration retinal ganglion cells (RGCs) axons the nerve. However little known about molecular mechanism...
The large conductance voltage- and Ca 2+ -activated potassium (BK) channel has been suggested to play an important role in the signal transduction process of cochlear inner hair cells. BK channels have shown be composed pore-forming α-subunit coexpressed with auxiliary β1-subunit. Analyzing hearing function phenotype α-(BKα –/– ) β1-subunit (BKβ1 knockout mice, we demonstrate normal structure BKβ1 mice. During first 4 postnatal weeks also, BKα mice most surprisingly did not show any obvious...
Spinocerebellar ataxia type 3 (SCA3) is an autosomal dominantly inherited neurodegenerative disorder caused by the expansion of a CAG repeat in MJD1 gene resulting expanded polyglutamine ataxin-3 protein. To study course disease, we generated transgenic mice for SCA3 using full-length constructs containing 15, 70, or 148 repeats, respectively. Control (15 CAGs) were phenotypically normal and had no neuropathological findings. However, with repeats severely affected strong neurological...
Mutations in the DFNB31 gene encoding PDZ scaffold protein whirlin are causative for hearing loss man and mouse. Whirlin is known to be essential elongation process of stereocilia sensory hair cells inner ear, though its complete spatial temporal expression patterns remained elusive. Here, we demonstrate that, embryonic development, not only expressed but also developing brain retina. Various isoforms widely differentially expressed, provide evidence that directly associates with USH2A...
Usher syndrome (USH) is the most frequent cause of combined deaf-blindness in man. USH clinically and genetically heterogeneous with at least 11 chromosomal loci assigned to three types ( USH1A-G , USH2A-C USH3A ). Although different exhibit almost same phenotype human, identified genes encode for proteins which belong very protein classes families. We others recently reported that scaffold harmonin USH1C -gene product) integrates all USH1 molecules a USH1-protein network. Here, we...
Tinnitus is proposed to be caused by decreased central input from the cochlea, followed increased spontaneous and evoked subcortical activity that interpreted as compensation for responsiveness of auditory circuits. We compared equally noise exposed rats separated into groups with without tinnitus differences in brain relative degree deafferentation periphery. analyzed (1) number CtBP2/RIBEYE-positive particles ribbon synapses inner hair cell (IHC) a measure deafferentation; (2) fine...
Hair cells of the mammalian cochlea are specialized for dynamic coding sound stimuli. The transduction waves into electrical signals depends upon mechanosensitive hair bundles that project from cell's apical surface. Each stereocilium within a bundle is composed uniformly polarized and tightly packed actin filaments. Several stereociliary proteins have been shown to be associated with development function known cause deafness in mice humans when mutated. growth stereociliar core dynamically...
A dramatic shift in societal demographics will lead to rapid growth the number of older people with hearing deficits. Poorer performance suprathreshold speech understanding and temporal processing age has been previously linked progressing inner hair cell (IHC) synaptopathy that precedes age-dependent elevation auditory thresholds. We compared central sound responsiveness after acoustic trauma young, middle-aged, rats. demonstrate IHC progresses from middle onward threshold becomes elevated...
The First International Conference on Hyperacusis gathered over 100 scientists and health care professionals in London, UK. Key conclusions from the conference included: (1) is characterized by reduced tolerance of sound that has perceptual, psychological social dimensions; (2) there a growing awareness children as well adults experience symptoms hyperacusis or misophonia; (3) exact mechanisms give rise to are not clear, but available evidence suggests functional changes within central...
Both a genetic or acquired neonatal thyroid hormone (TH) deficiency may result in profound mental disability that is often accompanied by deafness. The existence of various TH-sensitive periods during inner ear development and general success delayed, corrective TH treatment was investigated treating pregnant lactating rats with the goitrogen methimazole (MMI). We observed for establishment normal hearing ability, maternal TH, before fetal gland function on estrus days 17–18, obviously not...
In the mature cochlea, inner hair cells (IHCs) transduce acoustic signals into receptor potentials, communicating to brain by synaptic contacts with afferent fibers. Before onset of hearing, a transient efferent innervation is found on IHCs, mediated nicotinic cholinergic that may contain both α9 and α10 subunits. Calcium influx through activates calcium-dependent (SK2-containing) potassium channels. This inhibitory synapse thought disappear after hearing [after postnatal day 12 (P12)]. We...
Cochlear inner hair cells (IHCs) transduce sound-induced vibrations into a receptor potential (RP) that controls afferent synaptic activity and, consequently, frequency and timing of action potentials in the postsynaptic auditory neurons. The RP is thought to be shaped by two voltage-dependent K+ conductances, I(K,f) I(K,s), are carried large-conductance Ca2+- (BK)- K(V)-type channels. Using whole-cell voltage-clamp recordings acutely isolated mouse cochlea, we show IHCs display an...
Members of the neurotrophin gene family and their high-affinity Trk receptors control innervation cochlea during embryonic development. Lack signalling in has been well documented for early postnatal animals, resulting a loss cochlear sensory neurones region-specific reduction target along tonotopic axis. However, how reduced affects mature is currently unknown. Here, we have analysed consequences lack TrkB receptor its ligand, brain-derived neurotrophic factor (Bdnf), late or adult using...
The mammalian cochlea is specialized to recognize and process complex auditory signals with remarkable acuity temporal precision over a wide frequency range. quality of the information relayed afferent fibers mainly depends on transfer characteristics inner hair cell (IHC) ribbon synapses. To investigate biophysical properties synaptic machinery, we measured changes in membrane capacitance (Δ C m ) low-frequency (apical region, ∼300 Hz) high-frequency (basal, ∼30 kHz) gerbil IHCs maintained...
Mammalian cochlear inner hair cells (IHCs) are specialized to process developmental signals during immature stages and sound stimuli in adult animals. These conveyed onto auditory afferent nerve fibres. Neurotransmitter release at IHC ribbon synapses is controlled by L-type Ca(V)1.3 Ca(2+) channels, the biophysics of which still unknown native mammalian cells. We have investigated localization elementary properties channels mouse IHCs under near-physiological recording conditions. cell...
Otoferlin has been proposed to be the Ca2+ sensor in hair cell exocytosis, compensating for classical synaptic fusion proteins synaptotagmin-1 and synaptotagmin-2. In present study, yeast two-hybrid assays reveal myosin VI as a novel otoferlin binding partner. Co-immunoprecipitation assay co-expression suggest an interaction of both within basolateral part inner cells (IHCs). Comparison mutants mutant mice indicates non-complementary complementary roles maturation: (i) IHCs from exhibited...