A5064834609- Retinal Development and Disorders
- Retinal Diseases and Treatments
- melanin and skin pigmentation
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- Retinal and Macular Surgery
- Retinal Imaging and Analysis
- Photoreceptor and optogenetics research
- Connexins and lens biology
- Neurobiology and Insect Physiology Research
- Antioxidant Activity and Oxidative Stress
- Retinopathy of Prematurity Studies
- Ocular Oncology and Treatments
- Biochemical Analysis and Sensing Techniques
- Retinoids in leukemia and cellular processes
- RNA regulation and disease
- Photochromic and Fluorescence Chemistry
- Virus-based gene therapy research
- Trace Elements in Health
- Angiogenesis and VEGF in Cancer
- Neuroscience and Neuropharmacology Research
- Dye analysis and toxicity
- Circadian rhythm and melatonin
- Intraocular Surgery and Lenses
- Mitochondrial Function and Pathology
STZ eyetrial
2014-2024
University of Tübingen
2004-2022
In-Q-Tel
2012-2017
Universitätsklinikum Tübingen
2005-2016
Centre for Sight
2012-2015
Universitätsklinik für Augenheilkunde
2008
TH Bingen University of Applied Sciences
2003-2007
Ophthalmology Associates (United States)
2007
University of Cologne
1997-2003
University Hospital Cologne
1997-2002
Diabetic retinopathy is a leading cause of adult vision loss and blindness. Much the retinal damage that characterizes disease results from vascular leakage nonperfusion. leakage, capillary nonperfusion, endothelial cell are temporary spatially associated with leukocyte stasis in early experimental diabetes. Retinal leukostasis increases within days developing diabetes correlates increased expression intercellular adhesion molecule-1 (ICAM-1) CD18. Mice deficient genes encoding for molecules...
This work presents a combined light and electron microscopical approach to investigate the initial breakdown of retinal pigment epithelium (RPE) choriocapillaris (CC) in age-related macular degeneration (AMD). Perimacular sections 12 dry wet AMD eyes (82 ± 15 years) 7 age-matched controls (75 10 without pathology were investigated. Disease progression was classified into 5 stages concurrent CC breakdown. Special emphasis laid on transitions where intact CC–RPE–retina complexes went over...
Autosomal dominant optic atrophy (adOA) is a juvenile onset, progressive ocular disorder characterized by bilateral loss of vision, central visual field defects, colour vision disturbances, and disc pallor. adOA most frequently associated with mutations in OPA1 encoding dynamin-related large GTPase that localizes to mitochondria. Histopathological studies patients have shown degeneration retinal ganglion cells (RGCs) axons the nerve. However little known about molecular mechanism...
purpose. The penetration of intravitreally injected bevacizumab in its commercial formulation (Avastin; Roche, Grenzach, Germany) through the retina was studied, to determine whether a full-length antibody would be able penetrate as easily an fragment. methods. Six cynomolgus monkeys (Macaca fascicularis) were used this study. Two compositions intravitreal injection into right eyes performed: one with Avastin (group 1, four animals) and other labeled 125I 2, animal). animals group 1 killed...
To determine whether transplantation of embryonic stem (ES) cells into the subretinal space rhodopsin-knockout mice has a tumorigenic effect.Mouse ES-cell-derived neural precursor carrying sequence for green fluorescent protein (GFP) gene were grafted subretinally eyes rhodopsin(-/-) mice, whereas control animals underwent sham surgery. Eyes retrieved after 2, 4, and 8 weeks cell injection or surgery histologic analysis.Gross morphologic, histologic, immunohistochemical analysis at 2 4...
Since there is evidence that the Fc domain of antivascular endothelial growth factor drugs may cause unexpected consequences in retinal and choroidal vessels, effects intravitreal ranibizumab aflibercept on monkey eyes were investigated.Four cynomolgus monkeys intravitreally injected with 0.5 mg another four 2 aflibercept. Two untreated served as controls. Funduscopy, fluorescein angiography (FA), spectral-domain-optical coherence tomography (SD-OCT) measurement intraocular pressure (IOP)...
To investigate fundus autofluorescence (AF) characteristics in the Abca4(-/-) mouse, an animal model for AMD and Stargardt disease, to correlate findings with functional, structural, biochemical assessments.Blue (488 nm) near-infrared (790 AF images were quantitatively qualitatively analyzed pigmented mice wild type (WT) controls vivo. Functional, assessments included electroretinography (ERG), light electron microscopic analysis, A2E quantification. All performed across age groups.In mice,...
To determine the potential of adenovirally transduced bone marrow stromal cells (BMSCs) to differentiate into retinal pigment epithelial-like and evaluabe possible rescue effects after transplantation retinas Royal College Surgeons (RCS) rats.Through a high-capacity adenoviral vector expressing either green fluorescent protein (GFP) or epithelial-derived factor (PEDF), rat MSCs were in vitro before subretinal Wistar rats or, alternatively, RCS rats. Two months cell injection, killed eyes...
The main disease features of autosomal dominant optic atrophy (ADOA) are a bilateral reduction visual acuity, cecocentral scotoma, and frequently tritanopia, which have been ascribed to progressive loss retinal ganglion cells (RGCs) subsequent degeneration the nerve. disease-causing gene is OPA1. Here, we examine mouse carrying pathogenic mutation in Opa1 by electrophysiological measurements assess fate RGCs.Two-year-old animals underwent full examination electroretinography (ERG) visually...
Neuromelanin (NM) is a compound which highly accumulates mainly in catecholamine neurons of the substantia nigra (SN), and contained organelles (NM-containing organelles) with lipid bodies proteins. These selectively degenerate Parkinson's disease NM can play either protective or toxic role. NM-containing SN were investigated by Analytical Electron Microscopy (AEM) Nano-Secondary Ion Mass Spectrometry (NanoSIMS) within human tissue sections respect to ultrastructure elemental composition....
It is textbook knowledge that human infective forms of Trypanosoma brucei, the causative agent sleeping sickness, enter brain across blood-brain barrier after an initial phase weeks (rhodesiense) or months (gambiense) in blood. Based on our results using animal model, both statements seem questionable. As we and others have shown, first infection relevant crossing blood border occurs via choroid plexus, i.e. blood-CSF barrier. In addition, counting trypanosomes blood-free CSF obtained by...
PURPOSE. The correct orientation of retinal pigment epithelium (RPE) cells is necessary for the integrity and proper function retina. For transplantation RPE/iris (IPE) grafts to subretinal space in age-related macular degeneration, this cellular most effectively provided by a membranous support. goal study was establish an autologous or homologous membrane as substratum growth RPE/IPE. METHODS. Porcine bovine RPE IPE were placed primary culture on dissected sheet (5 × 5 mm) porcine...