A5003034830- Retinal Development and Disorders
- Retinal Diseases and Treatments
- Glaucoma and retinal disorders
- Retinal and Optic Conditions
- melanin and skin pigmentation
- Retinal Imaging and Analysis
- Ocular Oncology and Treatments
- Retinopathy of Prematurity Studies
- Retinal and Macular Surgery
- Circadian rhythm and melatonin
- Axon Guidance and Neuronal Signaling
- Lung Cancer Diagnosis and Treatment
- Nerve injury and regeneration
- Angiogenesis and VEGF in Cancer
- Adenosine and Purinergic Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Photochromic and Fluorescence Chemistry
- Neurogenesis and neuroplasticity mechanisms
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Trace Elements in Health
- Drug-Induced Ocular Toxicity
- Glioma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Lung Cancer Treatments and Mutations
STZ eyetrial
2012-2024
University of Tübingen
2009-2022
Centre for Sight
2012-2015
In-Q-Tel
2012
University of Geneva
2010
Immunologie, Immunopathologie et Chimie Thérapeutique
2002
Hôpital Civil, Strasbourg
2001
This work presents a combined light and electron microscopical approach to investigate the initial breakdown of retinal pigment epithelium (RPE) choriocapillaris (CC) in age-related macular degeneration (AMD). Perimacular sections 12 dry wet AMD eyes (82 ± 15 years) 7 age-matched controls (75 10 without pathology were investigated. Disease progression was classified into 5 stages concurrent CC breakdown. Special emphasis laid on transitions where intact CC–RPE–retina complexes went over...
purpose. The penetration of intravitreally injected bevacizumab in its commercial formulation (Avastin; Roche, Grenzach, Germany) through the retina was studied, to determine whether a full-length antibody would be able penetrate as easily an fragment. methods. Six cynomolgus monkeys (Macaca fascicularis) were used this study. Two compositions intravitreal injection into right eyes performed: one with Avastin (group 1, four animals) and other labeled 125I 2, animal). animals group 1 killed...
Since there is evidence that the Fc domain of antivascular endothelial growth factor drugs may cause unexpected consequences in retinal and choroidal vessels, effects intravitreal ranibizumab aflibercept on monkey eyes were investigated.Four cynomolgus monkeys intravitreally injected with 0.5 mg another four 2 aflibercept. Two untreated served as controls. Funduscopy, fluorescein angiography (FA), spectral-domain-optical coherence tomography (SD-OCT) measurement intraocular pressure (IOP)...
Long-term changes in lung cancer (LC) patients are difficult to evaluate. We report results from the French KBP-2020 real-life cohort.KBP-2020 was a prospective cohort that included all diagnosed with LC 2020, nonacademic public hospital France. Patient and tumour characteristics were described compared similarly designed cohorts 2000 2010.In 82 centers 8,999 LC. The proportion of women increased: 34·6% (3114/8999) to, 24·3% (1711/7051) 16·0% (904/5667) 2010 (p<0·0001). non-smokers higher...
The repulsive guidance molecule (RGM) is involved in the formation of central nervous system (CNS) during development by modulating growing axons. However, a role RGM CNS injury remains to be established. We studied expression spinal cord rats with (SCI). After SCI, RGM+ cells accumulated lesions and peri-lesional areas. During first days after was confined neurons, ballooned neurite fibers/retraction bulbs, smooth muscle/endothelial cells, leucocytes infiltrating lesion. Lesional frequently...
The chemokine receptor CCR5 is well known for its function in immune cells; however, it also expressed the brain, where specific role remains to be elucidated. Because genetic factors may influence risk of developing cerebral ischaemia or affect clinical outcome, we have analysed experimental stroke.Permanent was performed by occlusion middle artery wild-type and CCR5-deficient mice. Locomotor behaviour, infarct size histochemical alterations were at different time points after occlusion.The...
Fundus autofluorescence is a non-invasive imaging technique in ophthalmology. Conventionally, short-wavelength (SW-AF) used for detection of lipofuscin, byproduct the visual cycle which accumulates with age or disease retinal pigment epithelium (RPE). Furthermore, near-infrared (NIR-AF) as marker RPE and choroidal melanin, but contribution lipofuscin to NIR-AF signal unclear.We employed fluorescence microscopy investigate properties melanosomes, melanolipofuscin granules histologic sections...
Stargardt disease (SD) is characterized by the accumulation of age-pigment lipofuscin in retinal pigment epithelium (RPE) and subsequent neuroretinal degeneration. The leads to vision loss early life. Here, we investigate age-dependent ultrastructural changes three SD mouse models: albino Abca4-/- pigmented Abca4-/-.Rdh8-/- mice. Since found indications for oxidative stress primarily mice, tested RPE melanin its antioxidative capabilities.SD eyes were investigated light, fluorescence...
<b>Background:</b> Bevacizumab is an antiangiogenic compound developed to target tumour vessels. Its off-label use in ophthalmology requires vitro testing on ocular cells. <b>Aim:</b> To quantify the antipermeability and antiproliferative effects of bevacizumab cultured choroidal endothelial cells (CECs). It was examined whether deep-freezing attenuates its activity. <b>Methods:</b> Porcine CECs were permeable insert systems. Permeability cell monolayers quantified by a fluorescent...
Background Optical coherence tomography (OCT) is an invaluable diagnostic tool for the detection and follow-up of retinal pathology in patients experimental disease models. However, as morphological structures layering health well their alterations are complex, segmentation procedures have not yet reached a satisfactory level performance. Therefore, raw images qualitative data commonly used clinical scientific reports. Here, we assess value OCT reflectivity profiles basis quantitative...
Abstract It is well established that autoreactive B cells undergo negative selection. This stands in paradox with the high frequency of so-called natural producing low affinity polyreactive autoantibodies recurrent specificities, suggesting these are selected on basis their autoreactivity. We previously described two transgenic mouse lines (with and without IgD) a human autoantibody (nAAb) binds ssDNA Fcγ. In absence IgG, nAAb-transgenic develop normally. By crossing mice animals expressing...
Scavenging of VEGF by specific antibodies is a promising way to treat ocular conditions connected with neovascularization. Intravitreal injections Avastin (bevacizumab) are performed frequently as treatment such conditions. In this study, the authors examine whether retinal function in wild-type mice affected an intravitreal injection Avastin.Electroretinography was four different experimental groups C57BL/6 before and 1, 4, 12, 25 days afterwards. The first group injected intravitreally...
Abstract Age‐related macular degeneration (AMD) is the leading cause of blindness in older people developed world while Stargardt's disease (SD) a juvenile and an orphan disease. Both diseases are untreatable marked by accumulation lipofuscin advancing to progressive deterioration retinal pigment epithelium (RPE) retina subsequent vision loss till blindness. We discovered that small molecule belonging tetrahydropyridoether class compounds, soraprazan renamed remofuscin, able remove existing...
Background Age-related macular degeneration (AMD) is associated with lipofuscin accumulation whereas the content of melanosomes decreases. Melanosomes are main storage zinc in pigmented tissues. Since elderly population, as most affected group for AMD, prone to deficit, we investigated chemical and ultrastructural effects deficiency rat eyes after a six-month penury diet. Methodology/Principal Findings Adult Long Evans (LE) rats were investigated. The control animals fed normal alimentation...
It is known that endothelial cells in the kidney are also strongly VEGF-dependent. Whether intravitreal drugs can be detected within glomeruli or affect VEGF glomerular podocytes not known. Therefore, aim of this pilot study was to investigate effects a single injection aflibercept and ranibizumab on monkeys.The kidneys eight cynomolgus monkeys, which were intravitreally injected either with 2 mg 0.5 ranibizumab, investigated one seven days after injection. Two animals served as controls....
Accumulation of lipofuscin in the retinal pigment epithelium (RPE) is a hallmark aging and associated with degeneration encountered age-related macular (AMD) Stargardt disease (SD). Currently, treatment for lipofuscin-induced unavailable. Here, we report that Remofuscin (INN: soraprazan, tetrahydropyridoether small molecule) reverses accumulation aged primary human RPE cells non-cytotoxic SD mouse vitro. In addition, show removal after single intravitreal injection results rescue from model...
Objective: Due to its low price, bevacizumab, which binds vascular endothelial growth factor, is currently used off-label for the treatment of over 50 different eye diseases and has been adopted worldwide despite absence serious preclinical data. This study examines effects intravitreal bevacizumab on monkey eyes with particular focus choroidal retinal vessels. Methods: Cynomolgus monkeys received an injection 1.25 mg or without 125I labeling. The were enucleated between 1 14 days after...
Stargardt disease (STGD1), known as inherited retinal dystrophy, is caused by ABCA4 mutations. The pigmented Abca4-/- mouse strain only reflects the early stage of STGD1 since it devoid degeneration. This blue light-illuminated model presented pigment epithelium (RPE) and photoreceptor degeneration which was similar to advanced phenotype. In contrast, wild-type mice showed no RPE after light illumination. mice, acute diminished mean autofluorescence (AF) intensity in both fundus...
Abstract Naturally occurring autoreactive B cells are thought to be physically eliminated or rendered functionally silent through different mechanisms of tolerance. However, multireactive low affinity natural autoantibody-producing seem escape these in normal adults and could constitute the cell pool from which pathological autoantibodies can emerge. To analyze this apparent paradox clonal tolerance theory, we have made two transgenic mouse lines (μk, μ∂k) producing a human autoantibody....