A5009783365- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Ion channel regulation and function
- Neuroendocrine regulation and behavior
- Neurotransmitter Receptor Influence on Behavior
- Stress Responses and Cortisol
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- Neuropeptides and Animal Physiology
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- CRISPR and Genetic Engineering
- Hepatitis B Virus Studies
- Virus-based gene therapy research
- Tryptophan and brain disorders
- Hypothalamic control of reproductive hormones
- RNA Research and Splicing
- Biochemical Analysis and Sensing Techniques
- Genetics and Neurodevelopmental Disorders
- Animal Genetics and Reproduction
- RNA Interference and Gene Delivery
- Anesthesia and Neurotoxicity Research
Max Planck Institute for Medical Research
2015-2024
Oslo University Hospital
2023
Neuroscience Institute
2023
Heidelberg University
1995-2022
Durham University
2022
Duke University
2020
Max Planck Society
2007-2018
Novem (Netherlands)
2008
Johannes Gutenberg University Mainz
2006
Leipzig University
2006
The N -methyl D-aspartate (NMDA) receptor subtype of glutamate-gated ion channels possesses high calcium permeability and unique voltage-dependent sensitivity to magnesium is modulated by glycine. Molecular cloning identified three complementary DNA species rat brain, encoding NMDA subunits NMDAR2A (NR2A), NR2B, NR2C, which are 55 70% identical in sequence. These structurally related, with less than 20% sequence identity, other excitatory amino acid subunits, including the subunit NMDAR1...
A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with use of a probe generated in polymerase chain reaction oligonucleotide primers based on peptide sequences purified protein. As would be predicted from cDNA sequence, LH-CG-R consists 26-residue signal peptide, 341-residue extracellular domain displaying an internal repeat structure characteristic members leucine-rich glycoprotein (LRG) family, and 333-residue region containing seven...
Gene-targeted mice lacking the L-alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunit GluR-A exhibited normal development, life expectancy, and fine structure of neuronal dendrites synapses. In hippocampal CA1 pyramidal neurons, GluR-A-/- showed a reduction in functional AMPA receptors, with remaining receptors preferentially targeted to Thus, soma-patch currents were strongly reduced, but glutamatergic synaptic unaltered; evoked dendritic spinous Ca2+ transients,...
The arginine residue at position 586 of the GluR-B subunit renders heteromeric α-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-sensitive glutamate receptor channels impermeable to calcium. codon for this is introduced precursor messenger RNA (pre-mRNA) stage by site-selective adenosine editing a glutamine codon. Heterozygous mice engineered gene targeting harbor an editing-incompetent allele synthesized unedited subunits and, in principal neurons and interneurons, expressed AMPA...
Cloned cDNA encoding the rat Sertoli cell receptor for FSH was isolated from a cognate library and functionally expressed in cultured mammalian cells. The (FSH-R), as predicted cDNA, is single 75K polypeptide with 348 residue extracellular domain which contains three N-linked glycosylation sites. This connected to structure containing seven putative transmembrane segments displays sequence similarity G protein-coupled receptors. Thus, FSH-R identical its structural design LH/CG (LH/CG-R)....
NMDA receptors, a class of glutamate-gated cation channels with high Ca2+ conductance, mediate fast transmission and plasticity central excitatory synapses. We show here that gene-targeted mice expressing receptors without the large intracellular C-terminal domain any one three NR2 subunits phenotypically resemble made deficient in particular subunit. Mice NR2B subunit C-terminally truncated form (NR2B(deltaC/deltaC) mice) die perinatally. NR2A(deltaC/deltaC) are viable but exhibit impaired...
Mice were generated expressing green fluorescent protein (GFP) under the control of gonadotropin-releasing hormone (GnRH) promoter. Green fluorescence was observed in, and restricted to, GnRH-immunopositive neuronal somata in olfactory bulb, ganglion terminale, septal nuclei, diagonal band Broca (DBB), preoptic area (POA), caudal hypothalamus, as well GnRH dendrites axons, including axon terminals median eminence organum vasculosum lamina terminalis (OVLT). Whole-cell recordings from...
Abstract Summary: By applying the mammalian codon usage to Cre recombinase, we improved expression, as determined by immunoblot and functional analysis, in three different cell lines. The (iCre) gene was also designed reduce high CpG content of prokaryotic coding sequence, thereby reducing chances epigenetic silencing mammals. Transgenic iCre expressing mice were obtained with good frequency, these loxP‐mediated DNA recombination observed all cells iCre. Moreover, fused two estrogen receptor...
The immediate early gene c-fos is part of the activator protein-1 transcription factor and has been postulated to participate in molecular mechanisms learning memory. To test this hypothesis vivo, we generated mice with a nervous system-specific knock-out using Cre-loxP system. Adult lacking c-Fos CNS (c-fosDeltaCNS) showed normal general emotional behavior but were specifically impaired hippocampus-dependent spatial associative tasks. These deficits correlated reduction long-term...
Abstract Astrocytic Ca 2+ signaling has been intensively studied in health and disease but not quantified during natural sleep. Here, we employ an activity-based algorithm to assess astrocytic signals the neocortex of awake naturally sleeping mice while monitoring neuronal activity, brain rhythms behavior. We show that exhibit distinct features across sleep-wake cycle are reduced sleep compared wakefulness. Moreover, increase precedes transitions from slow wave wakefulness, with a peak upon...
We have characterized a second member (delta‐2) of new class subunits for the ligand‐gated excitatory amino acid receptor superfamily. The sequence delta‐2 exhibits an average identity 25% and 18.5% to non‐NMDA NMDA subunits, respectively. rat gene is expressed predominantly in Purkinje cells cerebellum whereas only low levels delta‐1 transcripts are found adult brain. However, expression undergoes pronounced developmental peak, with particularly high mRNA caudate putamen late...
To study the replication strategy of human hepatitis B virus, 5' end RNA pregenome and initiation sites DNA plus minus strands have been mapped. The was found to be terminally redundant by 120 nucleotides; it is initiated within pre-C region may also function as mRNA for synthesis major core protein virus reverse transcriptase. strand direct repeat sequence DR1, contains a terminal redundancy up eight nucleotides, its does not require any template switch. primed short oligoribonucleotide...
The mammalian RNA-specific adenosine deaminases DRADA/dsRAD (alias ADAR) and RED1 ADARB1) have been implicated in the site-selective editing of brain-expressed pre-mRNAs for glutamate receptor subunits antigenomic RNA hepatitis delta virus. These enzymes are expressed many if not all tissues, predicting an as yet unappreciated significance deamination-mediated recoding gene transcripts organism. We now report molecular cloning cDNA RED2 ADARB2), a third member deaminase family rodent. is...